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The map label for this gene is amtB [H]

Identifier: 85375601

GI number: 85375601

Start: 2793668

End: 2794987

Strand: Direct

Name: amtB [H]

Synonym: ELI_13870

Alternate gene names: 85375601

Gene position: 2793668-2794987 (Clockwise)

Preceding gene: 85375600

Following gene: 85375602

Centisome position: 91.52

GC content: 65.61

Gene sequence:

>1320_bases
ATGATCCGCTCGCTATTTTGTAGCGCCGGCGCGCTGGCCTTCACGGGCCTCGTATCGACCGCTGCCATCGCCCAAGAAGC
CGCTGAAGCGGCGCCTGTCCCCAACCCCGGCAACAACGCATGGATGATGACGGCAACCGTCCTCGTCCTGCTGATGATCC
TGCCGGGCCTCGCGCTGTTCTACGGTGGCCTGACCCGGTCCAAGAACATGCTCAGCACCATGACCCAAATCGGCGCGACC
GCCGCACTGGCCATGCTGGTGTGGGTGATGTGGGGCTATTCGACCGCCTTCGGACCGGAAGGCAACGCCTTTTTCAGCTG
GGGGGTCCCGTTCCTCGCCGGGATCGACGCCTCCAGCACCGCTGCGACGTTCACCGACGAAGTGATCAGCGAATATGTCT
TCATCAGCTTCCAGATGACCTTTGCGGCCATCACCGCCGCGCTGATCCTGGGCGCGACGGCGGAACGGATGAAGTTCAGC
GCCGTGATGGCTTTCGTCCCCATCTGGCTGACGATCGTCTATTTCCCGATCGCCCACATGGTCTGGGCCGGGGGCGGCCT
GCTGTTCGAAGACGGCGCGCTCGATTTCGCCGGCGGCACGGTGGTGCACATCAACGCCGGTGTCTCGGGCCTCGTCCTCG
CCTACCTGCTCGGCAAGCGTCGCGGCTATCCGGCCGAACCGATGCCGCCGCACAGCCTCACGCTGACCATGGTCGGCACC
GGTCTGCTGTGGGTCGGCTGGTTCGGTTTCAACGCCGGCTCCGCGCTCGAAGCCGATGCCTCCGCCGGCCTCGCCATGAT
CAACACCTTCGTTGCCACGGCTGCCGCCGCGCTGACCTGGATGGTGATCGAGCGGGCTGCGGGTCACAAGGGCTCGGCGC
TGGGCTTCTGTTCGGGCGTCATCGCGGGCCTCGTCGCCGTAACCCCGGCTGCCGGCAATAGCGGACCCTTCGGTGCGATC
CTGCTGGGCATCCTCTCGTCGGCGGTCTGCTATTACTTCGTGGCCAAGGTGAAGACCAAGCTTGGCTACGACGACTCGCT
CGACGCCTTCGGCATCCACGGGATCGGCGGCATAGTCGGCGCGATCGGCACCGGCATCGTCTACCAGCCGTTCCTCGGCG
GCCCCGGCGACGGTTCCACCGCGCTCGGCGCCCAGCTCTGGACCCAGACCTTCGGCGTCTTGGTAACGATAGCCTGGGCT
GGCATCGGCACCCTGGTCGCCGCCTACATCGTCAAGCTCATCATCGGCCTCAGGGTCGACGAAGAGAGCGAAGTCGATGG
GCTCGACATCTCCGAACACGGAGAGCGGGCTTACAACTGA

Upstream 100 bases:

>100_bases
TCGGCGATGGCAAGATTTTCGTCCTCGACCTCGCCACCGCCACCCGCATCCGCACGGGTGAAACCGGCGAGACCGCACTG
TGACCCGGGGGAATACCACT

Downstream 100 bases:

>100_bases
CACCCGAATTGGCGGGCCGGGAGCTACCTCTCCCGCACCCGGCCCGCCAATCTTTGTTCCTCCTGCGAACAACCACCTCG
ATAGGGCCGGAGCGCGATGC

Product: ammonia permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIRSLFCSAGALAFTGLVSTAAIAQEAAEAAPVPNPGNNAWMMTATVLVLLMILPGLALFYGGLTRSKNMLSTMTQIGAT
AALAMLVWVMWGYSTAFGPEGNAFFSWGVPFLAGIDASSTAATFTDEVISEYVFISFQMTFAAITAALILGATAERMKFS
AVMAFVPIWLTIVYFPIAHMVWAGGGLLFEDGALDFAGGTVVHINAGVSGLVLAYLLGKRRGYPAEPMPPHSLTLTMVGT
GLLWVGWFGFNAGSALEADASAGLAMINTFVATAAAALTWMVIERAAGHKGSALGFCSGVIAGLVAVTPAAGNSGPFGAI
LLGILSSAVCYYFVAKVKTKLGYDDSLDAFGIHGIGGIVGAIGTGIVYQPFLGGPGDGSTALGAQLWTQTFGVLVTIAWA
GIGTLVAAYIVKLIIGLRVDEESEVDGLDISEHGERAYN

Sequences:

>Translated_439_residues
MIRSLFCSAGALAFTGLVSTAAIAQEAAEAAPVPNPGNNAWMMTATVLVLLMILPGLALFYGGLTRSKNMLSTMTQIGAT
AALAMLVWVMWGYSTAFGPEGNAFFSWGVPFLAGIDASSTAATFTDEVISEYVFISFQMTFAAITAALILGATAERMKFS
AVMAFVPIWLTIVYFPIAHMVWAGGGLLFEDGALDFAGGTVVHINAGVSGLVLAYLLGKRRGYPAEPMPPHSLTLTMVGT
GLLWVGWFGFNAGSALEADASAGLAMINTFVATAAAALTWMVIERAAGHKGSALGFCSGVIAGLVAVTPAAGNSGPFGAI
LLGILSSAVCYYFVAKVKTKLGYDDSLDAFGIHGIGGIVGAIGTGIVYQPFLGGPGDGSTALGAQLWTQTFGVLVTIAWA
GIGTLVAAYIVKLIIGLRVDEESEVDGLDISEHGERAYN
>Mature_439_residues
MIRSLFCSAGALAFTGLVSTAAIAQEAAEAAPVPNPGNNAWMMTATVLVLLMILPGLALFYGGLTRSKNMLSTMTQIGAT
AALAMLVWVMWGYSTAFGPEGNAFFSWGVPFLAGIDASSTAATFTDEVISEYVFISFQMTFAAITAALILGATAERMKFS
AVMAFVPIWLTIVYFPIAHMVWAGGGLLFEDGALDFAGGTVVHINAGVSGLVLAYLLGKRRGYPAEPMPPHSLTLTMVGT
GLLWVGWFGFNAGSALEADASAGLAMINTFVATAAAALTWMVIERAAGHKGSALGFCSGVIAGLVAVTPAAGNSGPFGAI
LLGILSSAVCYYFVAKVKTKLGYDDSLDAFGIHGIGGIVGAIGTGIVYQPFLGGPGDGSTALGAQLWTQTFGVLVTIAWA
GIGTLVAAYIVKLIIGLRVDEESEVDGLDISEHGERAYN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=430, Percent_Identity=50.2325581395349, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=27.4193548387097, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=28.604118993135, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=26.5734265734266, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=28.2973621103118, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17563228, Length=262, Percent_Identity=25.9541984732824, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=34.2995169082126, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=36.150234741784, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=31.6425120772947, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=26.6826923076923, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45345; Mature: 45345

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSLFCSAGALAFTGLVSTAAIAQEAAEAAPVPNPGNNAWMMTATVLVLLMILPGLALF
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGGLTRSKNMLSTMTQIGATAALAMLVWVMWGYSTAFGPEGNAFFSWGVPFLAGIDASST
HCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCC
AATFTDEVISEYVFISFQMTFAAITAALILGATAERMKFSAVMAFVPIWLTIVYFPIAHM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWAGGGLLFEDGALDFAGGTVVHINAGVSGLVLAYLLGKRRGYPAEPMPPHSLTLTMVGT
HHCCCCEEEECCCEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GLLWVGWFGFNAGSALEADASAGLAMINTFVATAAAALTWMVIERAAGHKGSALGFCSGV
CHHHHEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IAGLVAVTPAAGNSGPFGAILLGILSSAVCYYFVAKVKTKLGYDDSLDAFGIHGIGGIVG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AIGTGIVYQPFLGGPGDGSTALGAQLWTQTFGVLVTIAWAGIGTLVAAYIVKLIIGLRVD
HHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EESEVDGLDISEHGERAYN
CCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MIRSLFCSAGALAFTGLVSTAAIAQEAAEAAPVPNPGNNAWMMTATVLVLLMILPGLALF
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YGGLTRSKNMLSTMTQIGATAALAMLVWVMWGYSTAFGPEGNAFFSWGVPFLAGIDASST
HCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCC
AATFTDEVISEYVFISFQMTFAAITAALILGATAERMKFSAVMAFVPIWLTIVYFPIAHM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWAGGGLLFEDGALDFAGGTVVHINAGVSGLVLAYLLGKRRGYPAEPMPPHSLTLTMVGT
HHCCCCEEEECCCEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GLLWVGWFGFNAGSALEADASAGLAMINTFVATAAAALTWMVIERAAGHKGSALGFCSGV
CHHHHEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IAGLVAVTPAAGNSGPFGAILLGILSSAVCYYFVAKVKTKLGYDDSLDAFGIHGIGGIVG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AIGTGIVYQPFLGGPGDGSTALGAQLWTQTFGVLVTIAWAGIGTLVAAYIVKLIIGLRVD
HHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EESEVDGLDISEHGERAYN
CCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]