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The map label for this gene is gyrB [H]

Identifier: 85375173

GI number: 85375173

Start: 2361415

End: 2363958

Strand: Direct

Name: gyrB [H]

Synonym: ELI_11730

Alternate gene names: 85375173

Gene position: 2361415-2363958 (Clockwise)

Preceding gene: 85375172

Following gene: 85375174

Centisome position: 77.36

GC content: 62.66

Gene sequence:

>2544_bases
ATGAGCCAAGAGACCCCGGATACTCCCGAAGCAATTCCTGAAAAAGTCCGTCAGAACGGCGAATACGGCGCGGATTCGAT
CAAGGTCCTCAAGGGCCTCGATGCGGTGCGCAAGCGCCCCGGCATGTATATCGGCGATACCGACGATGGATCGGGCCTGC
ACCACATGGTGTTCGAAGTCTCCGACAATGCCATCGATGAGGCGCTGGCGGGGCATTGCGATCTCGTTTTGATCGAATTG
AACCCCGATGGTTCGGTTTCGGTCGAGGACAATGGCCGCGGCATTCCGGTCGACATGCACAAGGAAGAGGGCGTGTCGGC
GGCGGAAGTCATCATGACCCAGCTGCATGCGGGCGGGAAGTTCGAAAACACGTCCGATGACAACGCCTACAAGGTTTCGG
GCGGCTTGCACGGCGTCGGTGTGTCGGTGGTCAACGCGCTGTCGGAGTGGCTCGAACTCGTAATCTGGCGCGACGGCAAA
GAGCATTACATGCGCTTCGAGCATGGCGAAGCGGTCAAGAGCCTAGAAGTCACAGGCGACGCGCCCAGGGTCGATCAGAA
CCCGGACGACAATGGCTTCAAGAAGGGCACCCGTGTCACCTTCATGGCAAGCCACGATACCTTCAAGAACGTGACCGACT
TCGATTTCGAGAAACTCGAACACCGCTATCGCGAACTGGCATTCCTCAATTCGGGCGTCCGTATCCTGCTGCGCGACAAG
CGGCACGAGGACGTGCGCGAGCACGACATGTTCTACGAAGGCGGCATCGCGGCCTTCGTGAAATATCTCGACCGCAACAA
GCAGCCGCTGGTGGCCGAGCCGATCTCGGTTTCGGCGGAAAAGGACGGCATCGGCATCGACGTCGCGCTGCAGTGGAACG
ACAGCTATTACGAGAACGTCCTTACCTTCACCAACAACATCCCGCAGCGCGACGGCGGCACACACCTGGCCGCGTTTCGC
TCGGCGCTGACGCGCACGCTCAACAACTACGCAACCGCAAGCGGGCTCCTGAAGAAGGAAAAGGTCAGCCTGTCCGGTGA
GGACATGCGCGAAGGCCTGACCGCGATCGTCTCCGTCAAGCTTCCCGATCCCAAGTTCGGCAGCCAGACCAAGGACAAGC
TGGTCAGTTCCGAAGTGCGCCAGCCGCTCGAAAGCCTGATGGGCGAGAAGATGGGCGAATGGCTGGAGGAAAACCCCAAC
GACGCCAAGGCTATCGTCCAGAAGATCATCGATGCCGCCGCCGCGCGCGAGGCCGCGCGCAAGGCGCGTGAGATGAGCCG
CAAGGGCGCGATGTCCGTCGCGTCGCTGCCCGGCAAGCTGGCCGATTGCCAGGAACGCGATCCGGCCAAGTCCGAACTGT
TTCTGGTCGAGGGCGATTCCGCCGGCGGTTCGGCCAAGCAGGGCCGCGACCGCAAGACGCAGGCGATCCTGCCGTTGAAG
GGCAAGATCCTCAATGTAGAGCGCGCGCGGTTCGACCGGATCATCTCATCCAAGGAAGTCGGCACCCTGATCCAGGCCAT
GGGCACCGGCCTGCGCGACGAGTTCAACCTCGAAAAGCTGCGCTATCACAAGATCGTGATCATGACCGACGCCGATGTCG
ACGGCGCGCATATCCGCACGCTGCTGCTCACTTTCTTCCACCGGCAGATGCCGGAAATCGTCAAGGCCGGGCACCTCTTC
ATCGCCCAGCCGCCGCTTTACAAGGTCTCCAAGGGACGCAGCGAAGTCTATCTCAAGGACGATCGCGAGCTCGACCGCTA
CCTGATTTCCGGCGGTTTGCAGGGCCGCGTGCTCGACAGCGGCGGCACGCAGCGCAGCGGGGCCGAGCTGCAAACGCTGG
TCGACCATGCGCTGCGAGTGCGCAATCTGATCGGCTTCGCGCCGCGCAAATACAACGCCGCGATCATCGAAGCGATGGCA
CTGGCCGGCGTGTTCGACCCTGCCAACCGCGCGTCGGCAGAGGCGCTGGAACGTGCGGCGGAGCGGCTGCAGCTGGGCGA
TCCCGAAGCCGAATGGAGCGCGCGCCGGACCGACGATGGCGGGGTTGCCTTCGAGCGCGTATGGCGCGGCGTCACGGATG
CGCACCTGATCGAACCGAAGTTCCTCGAAAGCGCGGAAGCGCGCAAGCTGCACAGCGTCGCTGCCGAAGAGGCCGAGAGC
TACGAAGTTGCCGCCCGGCTGAAAAAACTGTCGAGCGGAGAGACCACCGCGCCGGATCCCGAAAGCGCCGACGAAACCGA
TGCCGACACGCCGAGCTTCACCGATGACGATGCCATCACCCGGCCGACCGAATTGCTCGACGCGGTGCTCGCGGCAGGTC
GCAAGGGCCTGTCGATCGCGCGCTACAAGGGCCTCGGCGAGATGAACGCAGAGCAATTGTGGGAGACGACGCTCGATCCC
GAAGCACGCATCCTGCTGCAGGTGAAGGTCGAAGACGCCGACGTGACCGACGAAATCTTCACCCGCCTGATGGGCGATGT
CGTCGAACCGCGGCGCGAGTTCATCCAGGACAATGCGCTCAACGTGGCGAACCTCGATGTTTAG

Upstream 100 bases:

>100_bases
CCACAAAGCCTTCTAGGCCCTCCGGACCGGTGGTGGAAAAAGCGATGCCTGGCCCATCGCAAAGCTTGGGAATCCCCCAC
CCGAAGCCTATATACTGGGC

Downstream 100 bases:

>100_bases
CACATCGGAAGCCGTCGGTGCCCAGAACGTCGCCACCCTCGACGTGTAGGGATTTGAACGAACTGTGTTAGCCTCCTATA
TCACCTGCAGAATGCAGATC

Product: type IIA topoisomeraseb subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 847; Mature: 846

Protein sequence:

>847_residues
MSQETPDTPEAIPEKVRQNGEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIEL
NPDGSVSVEDNGRGIPVDMHKEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELVIWRDGK
EHYMRFEHGEAVKSLEVTGDAPRVDQNPDDNGFKKGTRVTFMASHDTFKNVTDFDFEKLEHRYRELAFLNSGVRILLRDK
RHEDVREHDMFYEGGIAAFVKYLDRNKQPLVAEPISVSAEKDGIGIDVALQWNDSYYENVLTFTNNIPQRDGGTHLAAFR
SALTRTLNNYATASGLLKKEKVSLSGEDMREGLTAIVSVKLPDPKFGSQTKDKLVSSEVRQPLESLMGEKMGEWLEENPN
DAKAIVQKIIDAAAAREAARKAREMSRKGAMSVASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQGRDRKTQAILPLK
GKILNVERARFDRIISSKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIVKAGHLF
IAQPPLYKVSKGRSEVYLKDDRELDRYLISGGLQGRVLDSGGTQRSGAELQTLVDHALRVRNLIGFAPRKYNAAIIEAMA
LAGVFDPANRASAEALERAAERLQLGDPEAEWSARRTDDGGVAFERVWRGVTDAHLIEPKFLESAEARKLHSVAAEEAES
YEVAARLKKLSSGETTAPDPESADETDADTPSFTDDDAITRPTELLDAVLAAGRKGLSIARYKGLGEMNAEQLWETTLDP
EARILLQVKVEDADVTDEIFTRLMGDVVEPRREFIQDNALNVANLDV

Sequences:

>Translated_847_residues
MSQETPDTPEAIPEKVRQNGEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIEL
NPDGSVSVEDNGRGIPVDMHKEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELVIWRDGK
EHYMRFEHGEAVKSLEVTGDAPRVDQNPDDNGFKKGTRVTFMASHDTFKNVTDFDFEKLEHRYRELAFLNSGVRILLRDK
RHEDVREHDMFYEGGIAAFVKYLDRNKQPLVAEPISVSAEKDGIGIDVALQWNDSYYENVLTFTNNIPQRDGGTHLAAFR
SALTRTLNNYATASGLLKKEKVSLSGEDMREGLTAIVSVKLPDPKFGSQTKDKLVSSEVRQPLESLMGEKMGEWLEENPN
DAKAIVQKIIDAAAAREAARKAREMSRKGAMSVASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQGRDRKTQAILPLK
GKILNVERARFDRIISSKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIVKAGHLF
IAQPPLYKVSKGRSEVYLKDDRELDRYLISGGLQGRVLDSGGTQRSGAELQTLVDHALRVRNLIGFAPRKYNAAIIEAMA
LAGVFDPANRASAEALERAAERLQLGDPEAEWSARRTDDGGVAFERVWRGVTDAHLIEPKFLESAEARKLHSVAAEEAES
YEVAARLKKLSSGETTAPDPESADETDADTPSFTDDDAITRPTELLDAVLAAGRKGLSIARYKGLGEMNAEQLWETTLDP
EARILLQVKVEDADVTDEIFTRLMGDVVEPRREFIQDNALNVANLDV
>Mature_846_residues
SQETPDTPEAIPEKVRQNGEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLIELN
PDGSVSVEDNGRGIPVDMHKEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELVIWRDGKE
HYMRFEHGEAVKSLEVTGDAPRVDQNPDDNGFKKGTRVTFMASHDTFKNVTDFDFEKLEHRYRELAFLNSGVRILLRDKR
HEDVREHDMFYEGGIAAFVKYLDRNKQPLVAEPISVSAEKDGIGIDVALQWNDSYYENVLTFTNNIPQRDGGTHLAAFRS
ALTRTLNNYATASGLLKKEKVSLSGEDMREGLTAIVSVKLPDPKFGSQTKDKLVSSEVRQPLESLMGEKMGEWLEENPND
AKAIVQKIIDAAAAREAARKAREMSRKGAMSVASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQGRDRKTQAILPLKG
KILNVERARFDRIISSKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRTLLLTFFHRQMPEIVKAGHLFI
AQPPLYKVSKGRSEVYLKDDRELDRYLISGGLQGRVLDSGGTQRSGAELQTLVDHALRVRNLIGFAPRKYNAAIIEAMAL
AGVFDPANRASAEALERAAERLQLGDPEAEWSARRTDDGGVAFERVWRGVTDAHLIEPKFLESAEARKLHSVAAEEAESY
EVAARLKKLSSGETTAPDPESADETDADTPSFTDDDAITRPTELLDAVLAAGRKGLSIARYKGLGEMNAEQLWETTLDPE
ARILLQVKVEDADVTDEIFTRLMGDVVEPRREFIQDNALNVANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=577, Percent_Identity=24.9566724436742, Blast_Score=142, Evalue=2e-33,
Organism=Homo sapiens, GI19913406, Length=567, Percent_Identity=25.2204585537919, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI48994957, Length=840, Percent_Identity=56.547619047619, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=557, Percent_Identity=40.394973070018, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=552, Percent_Identity=24.2753623188406, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=515, Percent_Identity=23.8834951456311, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=144, Percent_Identity=32.6388888888889, Blast_Score=76, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=604, Percent_Identity=24.0066225165563, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17136538, Length=544, Percent_Identity=27.5735294117647, Blast_Score=146, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93416; Mature: 93285

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQETPDTPEAIPEKVRQNGEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEV
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEE
SDNAIDEALAGHCDLVLIELNPDGSVSVEDNGRGIPVDMHKEEGVSAAEVIMTQLHAGGK
CCHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCC
FENTSDDNAYKVSGGLHGVGVSVVNALSEWLELVIWRDGKEHYMRFEHGEAVKSLEVTGD
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHEEEECCC
APRVDQNPDDNGFKKGTRVTFMASHDTFKNVTDFDFEKLEHRYRELAFLNSGVRILLRDK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
RHEDVREHDMFYEGGIAAFVKYLDRNKQPLVAEPISVSAEKDGIGIDVALQWNDSYYENV
CHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEEEEEECCHHHHHH
LTFTNNIPQRDGGTHLAAFRSALTRTLNNYATASGLLKKEKVSLSGEDMREGLTAIVSVK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEEE
LPDPKFGSQTKDKLVSSEVRQPLESLMGEKMGEWLEENPNDAKAIVQKIIDAAAAREAAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KAREMSRKGAMSVASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQGRDRKTQAILPLK
HHHHHHHHCCCHHHHCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCC
GKILNVERARFDRIISSKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRT
CCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHH
LLLTFFHRQMPEIVKAGHLFIAQPPLYKVSKGRSEVYLKDDRELDRYLISGGLQGRVLDS
HHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEECC
GGTQRSGAELQTLVDHALRVRNLIGFAPRKYNAAIIEAMALAGVFDPANRASAEALERAA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ERLQLGDPEAEWSARRTDDGGVAFERVWRGVTDAHLIEPKFLESAEARKLHSVAAEEAES
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YEVAARLKKLSSGETTAPDPESADETDADTPSFTDDDAITRPTELLDAVLAAGRKGLSIA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEH
RYKGLGEMNAEQLWETTLDPEARILLQVKVEDADVTDEIFTRLMGDVVEPRREFIQDNAL
HHCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NVANLDV
CEECCCC
>Mature Secondary Structure 
SQETPDTPEAIPEKVRQNGEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEV
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEE
SDNAIDEALAGHCDLVLIELNPDGSVSVEDNGRGIPVDMHKEEGVSAAEVIMTQLHAGGK
CCHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCC
FENTSDDNAYKVSGGLHGVGVSVVNALSEWLELVIWRDGKEHYMRFEHGEAVKSLEVTGD
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHEEEECCC
APRVDQNPDDNGFKKGTRVTFMASHDTFKNVTDFDFEKLEHRYRELAFLNSGVRILLRDK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
RHEDVREHDMFYEGGIAAFVKYLDRNKQPLVAEPISVSAEKDGIGIDVALQWNDSYYENV
CHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEEEEEECCHHHHHH
LTFTNNIPQRDGGTHLAAFRSALTRTLNNYATASGLLKKEKVSLSGEDMREGLTAIVSVK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEEE
LPDPKFGSQTKDKLVSSEVRQPLESLMGEKMGEWLEENPNDAKAIVQKIIDAAAAREAAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KAREMSRKGAMSVASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQGRDRKTQAILPLK
HHHHHHHHCCCHHHHCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCC
GKILNVERARFDRIISSKEVGTLIQAMGTGLRDEFNLEKLRYHKIVIMTDADVDGAHIRT
CCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHH
LLLTFFHRQMPEIVKAGHLFIAQPPLYKVSKGRSEVYLKDDRELDRYLISGGLQGRVLDS
HHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEECC
GGTQRSGAELQTLVDHALRVRNLIGFAPRKYNAAIIEAMALAGVFDPANRASAEALERAA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ERLQLGDPEAEWSARRTDDGGVAFERVWRGVTDAHLIEPKFLESAEARKLHSVAAEEAES
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YEVAARLKKLSSGETTAPDPESADETDADTPSFTDDDAITRPTELLDAVLAAGRKGLSIA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEH
RYKGLGEMNAEQLWETTLDPEARILLQVKVEDADVTDEIFTRLMGDVVEPRREFIQDNAL
HHCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NVANLDV
CEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]