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The map label for this gene is flhA [H]

Identifier: 85375102

GI number: 85375102

Start: 2301457

End: 2302563

Strand: Direct

Name: flhA [H]

Synonym: ELI_11375

Alternate gene names: 85375102

Gene position: 2301457-2302563 (Clockwise)

Preceding gene: 85375101

Following gene: 85375103

Centisome position: 75.4

GC content: 61.52

Gene sequence:

>1107_bases
ATGAAAACCCGCGCTGCCGTCGCCTTTGAAGCCAAGAGGCCGCTCGAGATCGTCGAGCTCGACCTTGAAGGCCCCAAGGC
CGGCGAAGTTCTGGTCGAGATCATGGCGACCGGCATCTGCCACACCGATGCCTACACGCTCGACGGGCTTGACAGCGAAG
GAATTTTCCCGAGCATTCTCGGCCACGAAGGCTGCGGCGTGGTGCGCGAAGTCGGTGCGGGCGTGACCTCGGTCAAGCCG
GGCGATCACGTGATCCCGCTTTACACGCCCGAATGTCGCCAGTGCAAAATGTGCCTTTCGGGCAAGACCAACCTGTGCAG
CGCGATCCGCGCGACGCAGGGGCAAGGCCTGATGCCCGACGGCACCAGCCGCTTCAGCTACAAGGGAGAGACGATCTACC
ACTACATGGGTTGCTCGACCTTCTCGAACTTTACTGTGCTTCCGGAAATTGCCGTTGCCAAGATCCGCGAAGACGCGCCG
TTCGACACCACCTGCTATGTCGGCTGCGGCGTGACGACGGGCGTTGGTGCGGTGACCAATACCGCCAAGGTCCAGCCGGG
CGATAATGTCGTCGTTTTCGGCCTCGGCGGGATCGGGCTGAACGTGATCCAGGGCGCGAAGCTCGCGGGTGCAGACCGCA
TCGTCGGCGTCGATATCAACCCTTCGAAAGAGGAATGGGGCCGCAAGTTCGGGATGACCGATTTCGTCAATCCGAAGGAA
ACCTCAAACGTAGTTGAACACCTCGTCGAGATGCTCGACGGCGGGGCCGACTACAGCTTCGACTGCACCGGCAATACCGA
CGTGATGCGCGATGCGCTGGAATGCTGCCACAAGGGCTGGGGCACCAGCATCATCATCGGCGTGGCCGAAGCGGGCAAGG
AAATCGCCACCCGCCCGTTCCAGCTCGTGACGGGCCGCAATTGGCGCGGCACCGCGTTCGGCGGAGCCAAGGGGCGCACC
GATGTGCCGAAGATCGTCGACTGGTACATGACCGGAAAGATCGAAATCGACCCGATGATCACGCACCGATTGAGCCTCGA
CGAGATCAACAAAGGCTTCGACCTGATGCATGCGGGTGAGAGCATTCGCGCCGTAGTAGTCTATTGA

Upstream 100 bases:

>100_bases
TGTCGAAGGGCTGTACTTTCTTCTGGTGCATTGCATGCCAAAAAGAAGAGCAGGGCTTCGACAGGCTCAGCCCGAACTGA
TTTTTGGGAGATAGGCAAAT

Downstream 100 bases:

>100_bases
TGGCGGGCGCTGCCCCCACCAAAGTCGTGCTCGGTGAAAAGTTTGCCGCCTTCAGCGAGCAGTGGTCCCCCAAGATCGTC
GCCCGTTTCAACGGCAACGA

Product: alcohol dehydrogenase class III

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTRAAVAFEAKRPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSILGHEGCGVVREVGAGVTSVKP
GDHVIPLYTPECRQCKMCLSGKTNLCSAIRATQGQGLMPDGTSRFSYKGETIYHYMGCSTFSNFTVLPEIAVAKIREDAP
FDTTCYVGCGVTTGVGAVTNTAKVQPGDNVVVFGLGGIGLNVIQGAKLAGADRIVGVDINPSKEEWGRKFGMTDFVNPKE
TSNVVEHLVEMLDGGADYSFDCTGNTDVMRDALECCHKGWGTSIIIGVAEAGKEIATRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMTGKIEIDPMITHRLSLDEINKGFDLMHAGESIRAVVVY

Sequences:

>Translated_368_residues
MKTRAAVAFEAKRPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSILGHEGCGVVREVGAGVTSVKP
GDHVIPLYTPECRQCKMCLSGKTNLCSAIRATQGQGLMPDGTSRFSYKGETIYHYMGCSTFSNFTVLPEIAVAKIREDAP
FDTTCYVGCGVTTGVGAVTNTAKVQPGDNVVVFGLGGIGLNVIQGAKLAGADRIVGVDINPSKEEWGRKFGMTDFVNPKE
TSNVVEHLVEMLDGGADYSFDCTGNTDVMRDALECCHKGWGTSIIIGVAEAGKEIATRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMTGKIEIDPMITHRLSLDEINKGFDLMHAGESIRAVVVY
>Mature_368_residues
MKTRAAVAFEAKRPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSILGHEGCGVVREVGAGVTSVKP
GDHVIPLYTPECRQCKMCLSGKTNLCSAIRATQGQGLMPDGTSRFSYKGETIYHYMGCSTFSNFTVLPEIAVAKIREDAP
FDTTCYVGCGVTTGVGAVTNTAKVQPGDNVVVFGLGGIGLNVIQGAKLAGADRIVGVDINPSKEEWGRKFGMTDFVNPKE
TSNVVEHLVEMLDGGADYSFDCTGNTDVMRDALECCHKGWGTSIIIGVAEAGKEIATRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMTGKIEIDPMITHRLSLDEINKGFDLMHAGESIRAVVVY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.3079019073569, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=45.8445040214477, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=44.7154471544715, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=44.7154471544715, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.1351351351351, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.1351351351351, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=44.1734417344173, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=42.3913043478261, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=42.6592797783933, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=61.5176151761518, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI87082125, Length=381, Percent_Identity=27.2965879265092, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790045, Length=363, Percent_Identity=28.3746556473829, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=25.4847645429363, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI226510992, Length=363, Percent_Identity=24.7933884297521, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787863, Length=361, Percent_Identity=24.0997229916898, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87081918, Length=220, Percent_Identity=31.3636363636364, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786518, Length=366, Percent_Identity=25.9562841530055, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786825, Length=254, Percent_Identity=26.3779527559055, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788407, Length=217, Percent_Identity=26.7281105990783, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.9597855227882, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.6666666666667, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.8104956268222, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562584, Length=370, Percent_Identity=29.4594594594595, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988145, Length=370, Percent_Identity=27.2972972972973, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=361, Percent_Identity=26.0387811634349, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=58.4905660377358, Blast_Score=461, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6323099, Length=386, Percent_Identity=27.979274611399, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=29.0960451977401, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=29.0960451977401, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=372, Percent_Identity=26.8817204301075, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=369, Percent_Identity=25.4742547425474, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=370, Percent_Identity=24.3243243243243, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=23.9669421487603, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=56.25, Blast_Score=449, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39426; Mature: 39426

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAFEAKRPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSIL
CCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
GHEGCGVVREVGAGVTSVKPGDHVIPLYTPECRQCKMCLSGKTNLCSAIRATQGQGLMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
GTSRFSYKGETIYHYMGCSTFSNFTVLPEIAVAKIREDAPFDTTCYVGCGVTTGVGAVTN
CCCCEEECCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
TAKVQPGDNVVVFGLGGIGLNVIQGAKLAGADRIVGVDINPSKEEWGRKFGMTDFVNPKE
CCEECCCCCEEEEEECCCCHHHHCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCHH
TSNVVEHLVEMLDGGADYSFDCTGNTDVMRDALECCHKGWGTSIIIGVAEAGKEIATRPF
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHCCCE
QLVTGRNWRGTAFGGAKGRTDVPKIVDWYMTGKIEIDPMITHRLSLDEINKGFDLMHAGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHEEECEEEECCHHEEECCHHHHCCCCCEEECCC
SIRAVVVY
CEEEEEEC
>Mature Secondary Structure
MKTRAAVAFEAKRPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSIL
CCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
GHEGCGVVREVGAGVTSVKPGDHVIPLYTPECRQCKMCLSGKTNLCSAIRATQGQGLMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
GTSRFSYKGETIYHYMGCSTFSNFTVLPEIAVAKIREDAPFDTTCYVGCGVTTGVGAVTN
CCCCEEECCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
TAKVQPGDNVVVFGLGGIGLNVIQGAKLAGADRIVGVDINPSKEEWGRKFGMTDFVNPKE
CCEECCCCCEEEEEECCCCHHHHCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCHH
TSNVVEHLVEMLDGGADYSFDCTGNTDVMRDALECCHKGWGTSIIIGVAEAGKEIATRPF
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHCCCE
QLVTGRNWRGTAFGGAKGRTDVPKIVDWYMTGKIEIDPMITHRLSLDEINKGFDLMHAGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHEEECEEEECCHHEEECCHHHHCCCCCEEECCC
SIRAVVVY
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]