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The map label for this gene is ybfF [C]

Identifier: 85375043

GI number: 85375043

Start: 2238542

End: 2239417

Strand: Direct

Name: ybfF [C]

Synonym: ELI_11080

Alternate gene names: 85375043

Gene position: 2238542-2239417 (Clockwise)

Preceding gene: 85375037

Following gene: 85375044

Centisome position: 73.34

GC content: 62.79

Gene sequence:

>876_bases
ATGGATTTAATCGGCCCCACTTCGCAGAGCTTCATCAGCCAGCGCACCAAGCTGCACTACGCCGATTGGGGCAATCACGA
TGCCCCGCCGCTGATCCTGTTGCATGGCGGTCGCGATCACTGCCGCAGCTGGGACTGGATGGCGGAGAAGCTGCGCAAGA
ACTGGCACGTGATATGCCCCGACCTGCGCGGCCATGGCGACAGCGAGTGGACCAATACCGGGGTCTACCAGGTCAGCGGC
TATGTCTACGATCTGGCGCAGCTGATCCACCAATTGGAACTCGCGCCCGTCACCATCGTGGCGCATTCGCTCGGCGGCAA
TATCGCCACGCGCTATGCCGGGCTTTACCCTGAGAACTTGCGCAAGCTGGTCGCCATCGAAGGGCTCGGGCCGAGCCCCA
AGGTGATGGACAAGATGGCGGAAACGCCGTGGTACGAACACCGCAAGCAATGGATGGAGAAGAAGCGCGACGCTTCATCG
CGCCTGCCGCGCCGCTACGACACGTTCGAATCCGCGCTCGACCGGATGCACGAAGCCAACAGCTATCTGAGCCGCGAGCA
GGCCGAGCACCTGACCCGCCACGGTACGATCCGCAACGAGGACGGCACCTGGAGCTGGAAGTTCGATCCGCACCTGCACG
CCTGGCCGCCCCACGACCTGACCCATGCGCAGATCGAGGAACTATGGGGCCGTGTGAGCTGCCCAACCTTGCTGGTCTAT
GGCAATGACAGCTGGGCTTCGAACCCGGAGAAAGACGGGCGGATGAAGCACTTCGGCGACAACGTCAGCGTCAGCGCCTA
CGATCGCGCCGGGCACTGGGTGCATCACGACCGGTTCGATGATTTCCTCGCCGAGGTCGAAGCTTTCATCGCCTAG

Upstream 100 bases:

>100_bases
GATGTTGCGTCGCACCGTATCATGTCGGTAGCGGACCGCAACGCATTCCAAAGGCCGTAAAACACGCGGCGCATGGCAGG
ACAGGCATGGACCGCAGCTC

Downstream 100 bases:

>100_bases
GGCCTCATCGATTGACTACGCTGTTCCCGACAACCATAGCGACGCGCCATGGCTTCGGGACCTTCCGACATACTTCGGCG
TGCGCAGGCGCTGATCCAGT

Product: putative hydrolase

Products: NA

Alternate protein names: Atropine acylhydrolase; Atropinesterase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDLIGPTSQSFISQRTKLHYADWGNHDAPPLILLHGGRDHCRSWDWMAEKLRKNWHVICPDLRGHGDSEWTNTGVYQVSG
YVYDLAQLIHQLELAPVTIVAHSLGGNIATRYAGLYPENLRKLVAIEGLGPSPKVMDKMAETPWYEHRKQWMEKKRDASS
RLPRRYDTFESALDRMHEANSYLSREQAEHLTRHGTIRNEDGTWSWKFDPHLHAWPPHDLTHAQIEELWGRVSCPTLLVY
GNDSWASNPEKDGRMKHFGDNVSVSAYDRAGHWVHHDRFDDFLAEVEAFIA

Sequences:

>Translated_291_residues
MDLIGPTSQSFISQRTKLHYADWGNHDAPPLILLHGGRDHCRSWDWMAEKLRKNWHVICPDLRGHGDSEWTNTGVYQVSG
YVYDLAQLIHQLELAPVTIVAHSLGGNIATRYAGLYPENLRKLVAIEGLGPSPKVMDKMAETPWYEHRKQWMEKKRDASS
RLPRRYDTFESALDRMHEANSYLSREQAEHLTRHGTIRNEDGTWSWKFDPHLHAWPPHDLTHAQIEELWGRVSCPTLLVY
GNDSWASNPEKDGRMKHFGDNVSVSAYDRAGHWVHHDRFDDFLAEVEAFIA
>Mature_291_residues
MDLIGPTSQSFISQRTKLHYADWGNHDAPPLILLHGGRDHCRSWDWMAEKLRKNWHVICPDLRGHGDSEWTNTGVYQVSG
YVYDLAQLIHQLELAPVTIVAHSLGGNIATRYAGLYPENLRKLVAIEGLGPSPKVMDKMAETPWYEHRKQWMEKKRDASS
RLPRRYDTFESALDRMHEANSYLSREQAEHLTRHGTIRNEDGTWSWKFDPHLHAWPPHDLTHAQIEELWGRVSCPTLLVY
GNDSWASNPEKDGRMKHFGDNVSVSAYDRAGHWVHHDRFDDFLAEVEAFIA

Specific function: Alkaloid degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=275, Percent_Identity=25.4545454545455, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI23200012, Length=275, Percent_Identity=25.4545454545455, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI18777795, Length=184, Percent_Identity=27.1739130434783, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554012, Length=275, Percent_Identity=21.8181818181818, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17137390, Length=121, Percent_Identity=30.5785123966942, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.10 [H]

Molecular weight: Translated: 33624; Mature: 33624

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIGPTSQSFISQRTKLHYADWGNHDAPPLILLHGGRDHCRSWDWMAEKLRKNWHVICP
CCCCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHCCCEEECC
DLRGHGDSEWTNTGVYQVSGYVYDLAQLIHQLELAPVTIVAHSLGGNIATRYAGLYPENL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHEEHHHHCCCHHHHHCCCCHHHH
RKLVAIEGLGPSPKVMDKMAETPWYEHRKQWMEKKRDASSRLPRRYDTFESALDRMHEAN
HHHEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SYLSREQAEHLTRHGTIRNEDGTWSWKFDPHLHAWPPHDLTHAQIEELWGRVSCPTLLVY
HHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
GNDSWASNPEKDGRMKHFGDNVSVSAYDRAGHWVHHDRFDDFLAEVEAFIA
CCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLIGPTSQSFISQRTKLHYADWGNHDAPPLILLHGGRDHCRSWDWMAEKLRKNWHVICP
CCCCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHCCCEEECC
DLRGHGDSEWTNTGVYQVSGYVYDLAQLIHQLELAPVTIVAHSLGGNIATRYAGLYPENL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHEEHHHHCCCHHHHHCCCCHHHH
RKLVAIEGLGPSPKVMDKMAETPWYEHRKQWMEKKRDASSRLPRRYDTFESALDRMHEAN
HHHEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SYLSREQAEHLTRHGTIRNEDGTWSWKFDPHLHAWPPHDLTHAQIEELWGRVSCPTLLVY
HHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
GNDSWASNPEKDGRMKHFGDNVSVSAYDRAGHWVHHDRFDDFLAEVEAFIA
CCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA