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The map label for this gene is dapE

Identifier: 85374948

GI number: 85374948

Start: 2149318

End: 2150454

Strand: Reverse

Name: dapE

Synonym: ELI_10605

Alternate gene names: 85374948

Gene position: 2150454-2149318 (Counterclockwise)

Preceding gene: 85374950

Following gene: 85374947

Centisome position: 70.45

GC content: 65.79

Gene sequence:

>1137_bases
ATGAGCCAAGCCCTCGACTACGCCAAGCGCCTGATTGCCGCGCCCAGCGTCACGCCCGCCACCGGCGCGGTGTTCGACGA
AATGCAGGCGATGCTGGAGCCGCTCGGCTTCGCAGTGCATCGCTTCACGCGCGGTGAGGGCGAAGAGGGCAGCGACGAGG
CACCGGTCGAAAACCTGTTCGCGATCCGCCATGGGCCGGAAGGCAGCAAGCATTTCGCCTTTGCCGGGCATCTCGACGTG
GTGCCGCCGGGCGAGGGCTGGACCAGCGCGCCGTTCGAACCGGAAGAGCGCGGCGAGCTGCTCTATGGGCGCGGCGCGGT
CGATATGAAAGGGGCGATCGCTTGCATGGTCGATGCGGTGGCGAACGTGCCGCAAGAAGCGGGAACGATCAGCTTCATCA
TCACCGGCGACGAGGAAGGCCCCGCGTTGCACGGCACCCGTGCGCTGATCGACTATATGCGGTCGGAGGGTATCAAGCCC
GACCTGTGCCTCGTCGGCGAGCCGACCAGCGTCAACCGGCTGGGCGACATGATGAAAATCGGGCGGCGCGGCTCGGTCAA
TATCTGGCTCGAGGTCGAAGGGACGCAAGGCCACGTCGCCTACCCGCACCTCGCCGGCAATCCGCTGCCCGCAATGGTCG
AAATCCTGCGCGAGCTCAACAATCTCCCCCTAGACGAAGGCACCGACTGGTTCCAGCCGAGCAATCTCGAGATTACCGAG
ATCGATGTGCCCAACCGCGCGCATAACGTGATCCCGGCCAAGGCCAAGGCGCGGATCTCGATCCGTTTCAACGACACGCA
TTCGGGGGCGTCGCTGTCCAAGCAAGTGATCGAGATCGCCGAGAAGCATGGCGGCACCGCGCGCCCGGTCATCAGCGGCG
AACCGTTCCTGACCGAGCCGGGCGCGTTCTCGAGCATGATTGCCGCTGCCGTGAAGGCGGAAACCGCTATCGATCCCGAG
CCGTCGACCACCGGCGGCACCTCGGACGCACGCTTCCTGCGCAGCGTGTGTCCGGTGATCGAATTCGGCCTCTGCAACGC
CACGATGCACAAGCGCGACGAGGCCGTAGCCATGGCGGACCTCGATACGCTAAGCCGCATTTACGCGCGGGTGGCGCAGG
CGGCGCTCTCCTCCTGA

Upstream 100 bases:

>100_bases
GGCGCGTCGAACGGCGCGGGGTAGCCGGTCGCGTTGGTCTGGGGGATCGCGGCGAGATCGAGCTTGGGCATGGGGCACAC
TCCTGATAGCGACAGCCGGT

Downstream 100 bases:

>100_bases
TCTCCCTCGTCACCCCGGACTTGATCCGGGGTCCCGCTTTCTAGCGTTTCAACACCAGGTTTCAAAAAGCGGGACCCCGG
CTCGTGGGCCGGGGCGACGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSQALDYAKRLIAAPSVTPATGAVFDEMQAMLEPLGFAVHRFTRGEGEEGSDEAPVENLFAIRHGPEGSKHFAFAGHLDV
VPPGEGWTSAPFEPEERGELLYGRGAVDMKGAIACMVDAVANVPQEAGTISFIITGDEEGPALHGTRALIDYMRSEGIKP
DLCLVGEPTSVNRLGDMMKIGRRGSVNIWLEVEGTQGHVAYPHLAGNPLPAMVEILRELNNLPLDEGTDWFQPSNLEITE
IDVPNRAHNVIPAKAKARISIRFNDTHSGASLSKQVIEIAEKHGGTARPVISGEPFLTEPGAFSSMIAAAVKAETAIDPE
PSTTGGTSDARFLRSVCPVIEFGLCNATMHKRDEAVAMADLDTLSRIYARVAQAALSS

Sequences:

>Translated_378_residues
MSQALDYAKRLIAAPSVTPATGAVFDEMQAMLEPLGFAVHRFTRGEGEEGSDEAPVENLFAIRHGPEGSKHFAFAGHLDV
VPPGEGWTSAPFEPEERGELLYGRGAVDMKGAIACMVDAVANVPQEAGTISFIITGDEEGPALHGTRALIDYMRSEGIKP
DLCLVGEPTSVNRLGDMMKIGRRGSVNIWLEVEGTQGHVAYPHLAGNPLPAMVEILRELNNLPLDEGTDWFQPSNLEITE
IDVPNRAHNVIPAKAKARISIRFNDTHSGASLSKQVIEIAEKHGGTARPVISGEPFLTEPGAFSSMIAAAVKAETAIDPE
PSTTGGTSDARFLRSVCPVIEFGLCNATMHKRDEAVAMADLDTLSRIYARVAQAALSS
>Mature_377_residues
SQALDYAKRLIAAPSVTPATGAVFDEMQAMLEPLGFAVHRFTRGEGEEGSDEAPVENLFAIRHGPEGSKHFAFAGHLDVV
PPGEGWTSAPFEPEERGELLYGRGAVDMKGAIACMVDAVANVPQEAGTISFIITGDEEGPALHGTRALIDYMRSEGIKPD
LCLVGEPTSVNRLGDMMKIGRRGSVNIWLEVEGTQGHVAYPHLAGNPLPAMVEILRELNNLPLDEGTDWFQPSNLEITEI
DVPNRAHNVIPAKAKARISIRFNDTHSGASLSKQVIEIAEKHGGTARPVISGEPFLTEPGAFSSMIAAAVKAETAIDPEP
STTGGTSDARFLRSVCPVIEFGLCNATMHKRDEAVAMADLDTLSRIYARVAQAALSS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=378, Percent_Identity=38.8888888888889, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1790395, Length=163, Percent_Identity=30.0613496932515, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789236, Length=273, Percent_Identity=25.2747252747253, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ERYLH (Q2N7X8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459010.1
- ProteinModelPortal:   Q2N7X8
- STRING:   Q2N7X8
- MEROPS:   M20.010
- GeneID:   3868909
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_10605
- NMPDR:   fig|314225.3.peg.906
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q2N7X8
- ProtClustDB:   PRK13009
- BioCyc:   ELIT314225:ELI_10605-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40418; Mature: 40287

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 79-79 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALDYAKRLIAAPSVTPATGAVFDEMQAMLEPLGFAVHRFTRGEGEEGSDEAPVENLF
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHE
AIRHGPEGSKHFAFAGHLDVVPPGEGWTSAPFEPEERGELLYGRGAVDMKGAIACMVDAV
EEECCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
ANVPQEAGTISFIITGDEEGPALHGTRALIDYMRSEGIKPDLCLVGEPTSVNRLGDMMKI
HCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
GRRGSVNIWLEVEGTQGHVAYPHLAGNPLPAMVEILRELNNLPLDEGTDWFQPSNLEITE
CCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IDVPNRAHNVIPAKAKARISIRFNDTHSGASLSKQVIEIAEKHGGTARPVISGEPFLTEP
ECCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
GAFSSMIAAAVKAETAIDPEPSTTGGTSDARFLRSVCPVIEFGLCNATMHKRDEAVAMAD
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHH
LDTLSRIYARVAQAALSS
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQALDYAKRLIAAPSVTPATGAVFDEMQAMLEPLGFAVHRFTRGEGEEGSDEAPVENLF
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHE
AIRHGPEGSKHFAFAGHLDVVPPGEGWTSAPFEPEERGELLYGRGAVDMKGAIACMVDAV
EEECCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
ANVPQEAGTISFIITGDEEGPALHGTRALIDYMRSEGIKPDLCLVGEPTSVNRLGDMMKI
HCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
GRRGSVNIWLEVEGTQGHVAYPHLAGNPLPAMVEILRELNNLPLDEGTDWFQPSNLEITE
CCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IDVPNRAHNVIPAKAKARISIRFNDTHSGASLSKQVIEIAEKHGGTARPVISGEPFLTEP
ECCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
GAFSSMIAAAVKAETAIDPEPSTTGGTSDARFLRSVCPVIEFGLCNATMHKRDEAVAMAD
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHH
LDTLSRIYARVAQAALSS
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA