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The map label for this gene is dctA [H]

Identifier: 85374947

GI number: 85374947

Start: 2147895

End: 2149133

Strand: Reverse

Name: dctA [H]

Synonym: ELI_10600

Alternate gene names: 85374947

Gene position: 2149133-2147895 (Counterclockwise)

Preceding gene: 85374948

Following gene: 85374946

Centisome position: 70.41

GC content: 64.0

Gene sequence:

>1239_bases
ATGCTTCGCACCTGGTTCGCAATTCCGCTGTGGCAGCGGGTCATCACCGCGCTCATCCTGGGCGTGCTGACCGGCTGGGC
CTGGGGGCCGGATGCGGAGAGCATCAAGTGGATCGGCGACTTTTTCATCAAGGCGATCAAGATGCTGGTCGTGCCGCTGA
TCTTCTTCAGCCTGGTCAGCGGGGTCGCCGCCATCGGCGACTTGCGCAAGCTGGGTGCGGTCGGCGGACGGGCGATGATC
CTGTTCGTCGTCACGGGCCAGATCGCGGTGTGGATGGGGCTGGGCCTCGGCACCTTTTTCGCGCCCGGCAGCGGAGTCGA
TACCAGCGCGATCGAGATGGGCGACGTGCCGGAGCCGAATGAAACCACCTTCGTCGACATGATCCTCTCCATCGTGCCGG
AAAGCCCGGTGCAGGTGATGGCGGACGTCGCGGTGCTGCCGCTGATCGTCTTTTCGTTGCTGATCGGCGCCGGCATCCTG
ATGGCGGAGAAGGACGGCGAACCCGTCCAGCGCGTGTTCGATAGCGGCGCGGTCATCATGCAGAAGGTCACGATGGTGGT
GATGGAGCTCACCCCGTTCGGCGTTTTCGCGCTGATGGCGTGGGTCGCGGGCACGCTGGGGCTGGACGCGTTGGTCAGCT
TGGCGCAGCTCGTCGCGCTCAATTATCTCGGCTGCCTGCTGATCATCTTCGTGATGTATTCGGCGATGATAAAATTCCTC
GCCCGCCTGCCGGTGCGGCATTTCTTCCGCGGCATCATCGACGCGATGGCGGTGAGCTATTCCACCGCCAGCTCCAACGC
GACGCTGCCGGTGACCCTACGCTGCGCCGAGCGCAACTTGGGCGTGTCCAATTCGGTCGCCAGCTTCGTGATCGCCCTGG
GCGCGACGGTGAACATGAACGGCACCGCGATGTATCTCGGCCTCGCCACCCTGTTCGGCGCGCAAATCTTCGGCGTCGAC
CTCAGCATGGGCGACTATTTCCTGATCTCGATCCTCGCCACGCTCGGCGCGATCGGCGCGGCGGGCATACCGGGCGCGGG
CCTGATCATGATGGCGCTGGTCTTCGGCGCAGTCGGCGTGCCGCTCGAAACCATCGCTTTCGTCGCCGGTGTGGACCGGA
TAATGGACATGATGCGCACTACAACGAATGTTTCGGGCGATGCGGCAGTGGCGACGACGGTGGCGGTGATGACGGGCGAG
ATCGACCGCGAGGAAATGATCTCGGCGGATGATGTTTAG

Upstream 100 bases:

>100_bases
TGGGCCGGGGCGACGAAATTGGGGTGCGATACGAGCATGCTTCCAATCGATGCCCGAATTGCCTAGCGTCCGCCCGACTG
ATTTGAGGGGGTCTCACCGC

Downstream 100 bases:

>100_bases
GCCGAACCTATGGAGAGCTCTTCACCTCCCAACATTCTTGCGATTTTCGAAGAGTTGGATGCACGAGTTGATAAGGAAGG
ATACACGTTTGCGAGTGAAG

Product: proton/glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLRTWFAIPLWQRVITALILGVLTGWAWGPDAESIKWIGDFFIKAIKMLVVPLIFFSLVSGVAAIGDLRKLGAVGGRAMI
LFVVTGQIAVWMGLGLGTFFAPGSGVDTSAIEMGDVPEPNETTFVDMILSIVPESPVQVMADVAVLPLIVFSLLIGAGIL
MAEKDGEPVQRVFDSGAVIMQKVTMVVMELTPFGVFALMAWVAGTLGLDALVSLAQLVALNYLGCLLIIFVMYSAMIKFL
ARLPVRHFFRGIIDAMAVSYSTASSNATLPVTLRCAERNLGVSNSVASFVIALGATVNMNGTAMYLGLATLFGAQIFGVD
LSMGDYFLISILATLGAIGAAGIPGAGLIMMALVFGAVGVPLETIAFVAGVDRIMDMMRTTTNVSGDAAVATTVAVMTGE
IDREEMISADDV

Sequences:

>Translated_412_residues
MLRTWFAIPLWQRVITALILGVLTGWAWGPDAESIKWIGDFFIKAIKMLVVPLIFFSLVSGVAAIGDLRKLGAVGGRAMI
LFVVTGQIAVWMGLGLGTFFAPGSGVDTSAIEMGDVPEPNETTFVDMILSIVPESPVQVMADVAVLPLIVFSLLIGAGIL
MAEKDGEPVQRVFDSGAVIMQKVTMVVMELTPFGVFALMAWVAGTLGLDALVSLAQLVALNYLGCLLIIFVMYSAMIKFL
ARLPVRHFFRGIIDAMAVSYSTASSNATLPVTLRCAERNLGVSNSVASFVIALGATVNMNGTAMYLGLATLFGAQIFGVD
LSMGDYFLISILATLGAIGAAGIPGAGLIMMALVFGAVGVPLETIAFVAGVDRIMDMMRTTTNVSGDAAVATTVAVMTGE
IDREEMISADDV
>Mature_412_residues
MLRTWFAIPLWQRVITALILGVLTGWAWGPDAESIKWIGDFFIKAIKMLVVPLIFFSLVSGVAAIGDLRKLGAVGGRAMI
LFVVTGQIAVWMGLGLGTFFAPGSGVDTSAIEMGDVPEPNETTFVDMILSIVPESPVQVMADVAVLPLIVFSLLIGAGIL
MAEKDGEPVQRVFDSGAVIMQKVTMVVMELTPFGVFALMAWVAGTLGLDALVSLAQLVALNYLGCLLIIFVMYSAMIKFL
ARLPVRHFFRGIIDAMAVSYSTASSNATLPVTLRCAERNLGVSNSVASFVIALGATVNMNGTAMYLGLATLFGAQIFGVD
LSMGDYFLISILATLGAIGAAGIPGAGLIMMALVFGAVGVPLETIAFVAGVDRIMDMMRTTTNVSGDAAVATTVAVMTGE
IDREEMISADDV

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=471, Percent_Identity=29.2993630573248, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI40254478, Length=448, Percent_Identity=30.1339285714286, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI21314632, Length=403, Percent_Identity=29.5285359801489, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI66773030, Length=392, Percent_Identity=31.3775510204082, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4827012, Length=290, Percent_Identity=34.1379310344828, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194239697, Length=144, Percent_Identity=45.1388888888889, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI5032093, Length=402, Percent_Identity=29.1044776119403, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=31.095406360424, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI223468564, Length=276, Percent_Identity=31.5217391304348, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI301601644, Length=170, Percent_Identity=35.8823529411765, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI262359914, Length=386, Percent_Identity=26.1658031088083, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1790514, Length=418, Percent_Identity=35.4066985645933, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1789947, Length=418, Percent_Identity=29.4258373205742, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788024, Length=410, Percent_Identity=26.8292682926829, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789473, Length=389, Percent_Identity=24.1645244215938, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71983106, Length=428, Percent_Identity=32.0093457943925, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71983099, Length=428, Percent_Identity=32.0093457943925, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71996953, Length=421, Percent_Identity=32.0665083135392, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193206505, Length=443, Percent_Identity=29.1196388261851, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537407, Length=400, Percent_Identity=30.25, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17541374, Length=441, Percent_Identity=28.7981859410431, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI193206654, Length=409, Percent_Identity=28.8508557457213, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24583025, Length=393, Percent_Identity=30.0254452926209, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137668, Length=393, Percent_Identity=30.0254452926209, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24583023, Length=393, Percent_Identity=30.0254452926209, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI281360483, Length=472, Percent_Identity=25.2118644067797, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17137666, Length=472, Percent_Identity=25.2118644067797, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI281360481, Length=472, Percent_Identity=25.2118644067797, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43410; Mature: 43410

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]