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The map label for this gene is nth [H]

Identifier: 85374945

GI number: 85374945

Start: 2146834

End: 2147484

Strand: Direct

Name: nth [H]

Synonym: ELI_10590

Alternate gene names: 85374945

Gene position: 2146834-2147484 (Clockwise)

Preceding gene: 228879505

Following gene: 85374949

Centisome position: 70.33

GC content: 62.21

Gene sequence:

>651_bases
ATGACCAAGGACCAGATTTTCGAATTCTTCCGGCGGCTGGCGGAAGACAATCCCGAGCCGGAGACCGAGCTGGAATATGG
CAATTGCTACCAGCTGGTGGTGGCCGTCGCGCTCTCCGCGCAGGCGACCGATGTGGGGGTCAACAAGGCCACCCGCGCGC
TGTTTGCGAAGGTCGAAACGCCCGCACAAATGATTGAACTCGGCCTCGACGGGCTGATCGAGCACATCAAGACCATCGGC
CTGTTCAATTCCAAGGCCAAAAACGTGATCGCGCTGAGCCAGTTGCTGATCGACGATTATGGCGGCGAGGTGCCGGACAC
GCGCGAGGACCTCGTGCGGCTGCCGGGAGTGGGCCGCAAGACCGCCAATGTGGTCTTGAACTGCTGGTTCAGGCAGGAAA
CCTTCGCGGTCGACACCCATATCCTCAGGGTCGGCAACCGCACCGGCCTCGCCAAGGGCAAGACGCCCGAGCAGGTCGAG
GCGAAGCTGGAAAAGCGCGTCCCCCAGCCCTTCCGCCTGCATGCGCATCACTGGCTGATCCTGCATGGCCGCTATGTTTG
CAAGGCGCGAACGCCGGAGTGCTGGCGGTGCAAGGTCGTGGATTTGTGCAGCTACCGGAAGAAGGTGCTGGAGAAGCCGA
AGGGGCGTTAG

Upstream 100 bases:

>100_bases
ATCTTCGCTCGCGGTGCGGTGAAGGCGGCGGAGTGGCTGATCGGCAAGCAGGCCGGTCGCTATGCGATGGCGGACGTGCT
GGGGCTCTGAGCAAGCTGCC

Downstream 100 bases:

>100_bases
GCGAGTCTGGCTACGATTCTTGCGATTTCGGGCTTGGTATATTGCTCAATCGGATCGAGCTCGTCAGTTTTGGGCGGCCA
CTCACCTGCATCGAATGCCT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTKDQIFEFFRRLAEDNPEPETELEYGNCYQLVVAVALSAQATDVGVNKATRALFAKVETPAQMIELGLDGLIEHIKTIG
LFNSKAKNVIALSQLLIDDYGGEVPDTREDLVRLPGVGRKTANVVLNCWFRQETFAVDTHILRVGNRTGLAKGKTPEQVE
AKLEKRVPQPFRLHAHHWLILHGRYVCKARTPECWRCKVVDLCSYRKKVLEKPKGR

Sequences:

>Translated_216_residues
MTKDQIFEFFRRLAEDNPEPETELEYGNCYQLVVAVALSAQATDVGVNKATRALFAKVETPAQMIELGLDGLIEHIKTIG
LFNSKAKNVIALSQLLIDDYGGEVPDTREDLVRLPGVGRKTANVVLNCWFRQETFAVDTHILRVGNRTGLAKGKTPEQVE
AKLEKRVPQPFRLHAHHWLILHGRYVCKARTPECWRCKVVDLCSYRKKVLEKPKGR
>Mature_215_residues
TKDQIFEFFRRLAEDNPEPETELEYGNCYQLVVAVALSAQATDVGVNKATRALFAKVETPAQMIELGLDGLIEHIKTIGL
FNSKAKNVIALSQLLIDDYGGEVPDTREDLVRLPGVGRKTANVVLNCWFRQETFAVDTHILRVGNRTGLAKGKTPEQVEA
KLEKRVPQPFRLHAHHWLILHGRYVCKARTPECWRCKVVDLCSYRKKVLEKPKGR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=56.4593301435407, Blast_Score=239, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.638418079096, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=26.7676767676768, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319304, Length=176, Percent_Identity=27.8409090909091, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.638418079096, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24481; Mature: 24349

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDQIFEFFRRLAEDNPEPETELEYGNCYQLVVAVALSAQATDVGVNKATRALFAKVET
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PAQMIELGLDGLIEHIKTIGLFNSKAKNVIALSQLLIDDYGGEVPDTREDLVRLPGVGRK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCH
TANVVLNCWFRQETFAVDTHILRVGNRTGLAKGKTPEQVEAKLEKRVPQPFRLHAHHWLI
HHHHHHHHEECCCHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEEE
LHGRYVCKARTPECWRCKVVDLCSYRKKVLEKPKGR
EECEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKDQIFEFFRRLAEDNPEPETELEYGNCYQLVVAVALSAQATDVGVNKATRALFAKVET
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PAQMIELGLDGLIEHIKTIGLFNSKAKNVIALSQLLIDDYGGEVPDTREDLVRLPGVGRK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCH
TANVVLNCWFRQETFAVDTHILRVGNRTGLAKGKTPEQVEAKLEKRVPQPFRLHAHHWLI
HHHHHHHHEECCCHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEEE
LHGRYVCKARTPECWRCKVVDLCSYRKKVLEKPKGR
EECEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA