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The map label for this gene is ubiE [H]

Identifier: 85374937

GI number: 85374937

Start: 2139705

End: 2140436

Strand: Reverse

Name: ubiE [H]

Synonym: ELI_10550

Alternate gene names: 85374937

Gene position: 2140436-2139705 (Counterclockwise)

Preceding gene: 85374942

Following gene: 85374936

Centisome position: 70.12

GC content: 61.89

Gene sequence:

>732_bases
ATGACCGATACCGTATCCTTCGGCTATGAAGAGGTAGCCCCCGAAGAAAAGACCGAGCGCGTGGGCGCGGTCTTCTCCAA
TGTCGCGGCGAAATACGACATCATGAACGACGCCATGTCGGGCGGCATGCACCGGCTGTGGAAGGATCGCTTCGTGCGCC
GGGTCAAGCCGCAACCGGGCGAAGCGGTGCTCGACATGGCGGGCGGCACGGGCGATATCGCTTTCCGCATGGCCGCGCGC
GGGGCGGAGGTCACCGTGGCCGACATCAACCAGGACATGCTCGACATCGGCGTCGAACGCGCGATGGATAAGGGGCTGAC
CGGCCTCGTCTGGTCGTGCCAGAACGCCGAGCAGCTCGACTATCTCGACCGGCAGTTCGATGCCTACACCATCGTTTTCG
GCATCCGCAACGTGACCCATATCGACAAGGCGCTGCGCGAGGCGCACCGCGTGCTCAAGCACGGCGGGCGCTTCTATTGT
ATGGAATTCAGCACCACGGAGTGGCCGGGCTTCAAGGAAATCTACGACCTCTACTCGCATGAGGTCATGCCGAAGATCGG
CAAGGCGATCGCCGATGACGAGGATAGCTATCGCTATCTCGCCGAGTCGATCCGTCGCTTCCCCAAGCCGGCGGAATTCG
AAGCGATGATCCGGGCGGCAGGATTCGCAAACACGCGCGTGGAGAAAATTCTGGGCGGCGCCGTGAACATTCATTCCGGC
TGGAAGATCTGA

Upstream 100 bases:

>100_bases
CGAGCCCGCGAACCGTTGTTTCGACTTCAGGTAACTCAGGCATTGCCGAACCTCCTAACACCCTTTGCCGGGGTAGCAAT
TGCCTCTAAGGCCGCACGCG

Downstream 100 bases:

>100_bases
CGCGCTCCCATGACGCGTCCCGTCACCCATATCTGGCGGCTCCTCAAATGGGGCCGCACTGCCGCCAAGCATGGGGCGCT
GCGCGGGATCGAGCGCGACC

Product: ubiquinone methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTDTVSFGYEEVAPEEKTERVGAVFSNVAAKYDIMNDAMSGGMHRLWKDRFVRRVKPQPGEAVLDMAGGTGDIAFRMAAR
GAEVTVADINQDMLDIGVERAMDKGLTGLVWSCQNAEQLDYLDRQFDAYTIVFGIRNVTHIDKALREAHRVLKHGGRFYC
MEFSTTEWPGFKEIYDLYSHEVMPKIGKAIADDEDSYRYLAESIRRFPKPAEFEAMIRAAGFANTRVEKILGGAVNIHSG
WKI

Sequences:

>Translated_243_residues
MTDTVSFGYEEVAPEEKTERVGAVFSNVAAKYDIMNDAMSGGMHRLWKDRFVRRVKPQPGEAVLDMAGGTGDIAFRMAAR
GAEVTVADINQDMLDIGVERAMDKGLTGLVWSCQNAEQLDYLDRQFDAYTIVFGIRNVTHIDKALREAHRVLKHGGRFYC
MEFSTTEWPGFKEIYDLYSHEVMPKIGKAIADDEDSYRYLAESIRRFPKPAEFEAMIRAAGFANTRVEKILGGAVNIHSG
WKI
>Mature_242_residues
TDTVSFGYEEVAPEEKTERVGAVFSNVAAKYDIMNDAMSGGMHRLWKDRFVRRVKPQPGEAVLDMAGGTGDIAFRMAARG
AEVTVADINQDMLDIGVERAMDKGLTGLVWSCQNAEQLDYLDRQFDAYTIVFGIRNVTHIDKALREAHRVLKHGGRFYCM
EFSTTEWPGFKEIYDLYSHEVMPKIGKAIADDEDSYRYLAESIRRFPKPAEFEAMIRAAGFANTRVEKILGGAVNIHSGW
KI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=274, Percent_Identity=47.0802919708029, Blast_Score=247, Evalue=7e-66,
Organism=Escherichia coli, GI48994983, Length=244, Percent_Identity=45.9016393442623, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17557039, Length=241, Percent_Identity=49.3775933609959, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6323526, Length=256, Percent_Identity=42.1875, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24641702, Length=257, Percent_Identity=49.0272373540856, Blast_Score=249, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27373; Mature: 27242

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTVSFGYEEVAPEEKTERVGAVFSNVAAKYDIMNDAMSGGMHRLWKDRFVRRVKPQPG
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAVLDMAGGTGDIAFRMAARGAEVTVADINQDMLDIGVERAMDKGLTGLVWSCQNAEQLD
CEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
YLDRQFDAYTIVFGIRNVTHIDKALREAHRVLKHGGRFYCMEFSTTEWPGFKEIYDLYSH
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
EVMPKIGKAIADDEDSYRYLAESIRRFPKPAEFEAMIRAAGFANTRVEKILGGAVNIHSG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEECCCC
WKI
CCC
>Mature Secondary Structure 
TDTVSFGYEEVAPEEKTERVGAVFSNVAAKYDIMNDAMSGGMHRLWKDRFVRRVKPQPG
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAVLDMAGGTGDIAFRMAARGAEVTVADINQDMLDIGVERAMDKGLTGLVWSCQNAEQLD
CEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
YLDRQFDAYTIVFGIRNVTHIDKALREAHRVLKHGGRFYCMEFSTTEWPGFKEIYDLYSH
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
EVMPKIGKAIADDEDSYRYLAESIRRFPKPAEFEAMIRAAGFANTRVEKILGGAVNIHSG
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCEECCCC
WKI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA