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The map label for this gene is iaaA [H]

Identifier: 85374893

GI number: 85374893

Start: 2096841

End: 2097953

Strand: Reverse

Name: iaaA [H]

Synonym: ELI_10330

Alternate gene names: 85374893

Gene position: 2097953-2096841 (Counterclockwise)

Preceding gene: 85374899

Following gene: 85374884

Centisome position: 68.73

GC content: 65.32

Gene sequence:

>1113_bases
ATGAATCGCATCAAACACACGCTCACCGCTGTTTCGACAGCTATATCCCTGTTTTTTGCCGCTCCACTGCTGGCGCAGGA
CGATTATGAGCCGATGGAGCCGCGATGGTCCTTCGCCATCCACGGCGGGGCGGGTACGATCGCGCGCGAGAACATGACGC
CGGAACAGGATGCCGAATATCGCGCAGCGCTGCAGCAGGCGCTCGATGCCGGGTCGGACATCCTCAAAAACGGTGGCAGC
GCAATGGACGCGGTGCTGGCGGCGATCGTGCTGCTGGAGGACGATCCCAAGTTCAACGCAGGGCGCGGCGCGGTCTTCAC
CTGGGAAGGCGAGAACGAGCTCGACGCCGCGCTGATGGACGGCAAGGATCGCAGCGCCGGGGCCGTAACCGGCGTCAAGG
CGATCAAGAACCCGATCCTGCTCGCACGCGAGGTGATGAAGGACGGTCGGCACGTCTTCCTGAGCGGCGCGGGAGCAGAG
GAATTCGCCGGAGAGCGCGGGCTTGAAACCGCCCCGCCGGAATGGTTCGCCACCGAAGCGCGGCGCGAAGCGCTGGAGCG
GCTCAAGGCGCGCCAGCTTTCTTCACTCGATGTCGATACCAAGTTCGGTACGGTCGGCGCGGTCGCGCTGGATCTTGATG
GGAACATGGTGGCGGGGACCTCGACCGGCGGGCTCACCGGCAAGCGCTGGGGCCGCATCGGGGATGTGCCGGTGCTCGGC
GCGGGCACCTATGCCGACAATCGCGATTGCGCGGTCTCGGCGACCGGGCAGGGCGAGTATTTCATCCGAGCGGTGGTCGG
TTACTCGATCTGCACCGGCCTGAAGCATAAGTGGCGCACGATGCTGGACGAGGCACAGGCTTCGGTGCCGCTAGACGATG
ACGGCAACCCCACCTTCATGGTTCACGCCAGCGAGATGTGGCTGGAGCAGAGCGACGTCCAGGCGGTTGCCGACGAAGTG
ATGGCCGACGTGAAGGACCTGGGCGGCACCGGCGGCGTTATCGTCGTCTCGCCCTTCGGCCCGGCGGTCTTCAGCTTCAA
TACGCCTGGCATGTATCGCGGCCGCGCCACCAGCGCCGGCGTCAACGAAGTGGCGATTTACGGCGACGAATAG

Upstream 100 bases:

>100_bases
AAGTAACGGATACCACAGGGGTCTTGCAGTTCCCAAGCGGCAATTGTCGCGCAGTGGATAAACGCTGGCGCCAGTGCAGG
CGAATGCTATCACTGCTGCC

Downstream 100 bases:

>100_bases
GGCCGGGCGCTCAGGCCGCGATCGGGGTAACCTCGTCCGGGCGGGTTGCGCGGCGGATAACATCGCCGAATCCATGCGCG
ATGCAATCGTGGTGCTTGTA

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNRIKHTLTAVSTAISLFFAAPLLAQDDYEPMEPRWSFAIHGGAGTIARENMTPEQDAEYRAALQQALDAGSDILKNGGS
AMDAVLAAIVLLEDDPKFNAGRGAVFTWEGENELDAALMDGKDRSAGAVTGVKAIKNPILLAREVMKDGRHVFLSGAGAE
EFAGERGLETAPPEWFATEARREALERLKARQLSSLDVDTKFGTVGAVALDLDGNMVAGTSTGGLTGKRWGRIGDVPVLG
AGTYADNRDCAVSATGQGEYFIRAVVGYSICTGLKHKWRTMLDEAQASVPLDDDGNPTFMVHASEMWLEQSDVQAVADEV
MADVKDLGGTGGVIVVSPFGPAVFSFNTPGMYRGRATSAGVNEVAIYGDE

Sequences:

>Translated_370_residues
MNRIKHTLTAVSTAISLFFAAPLLAQDDYEPMEPRWSFAIHGGAGTIARENMTPEQDAEYRAALQQALDAGSDILKNGGS
AMDAVLAAIVLLEDDPKFNAGRGAVFTWEGENELDAALMDGKDRSAGAVTGVKAIKNPILLAREVMKDGRHVFLSGAGAE
EFAGERGLETAPPEWFATEARREALERLKARQLSSLDVDTKFGTVGAVALDLDGNMVAGTSTGGLTGKRWGRIGDVPVLG
AGTYADNRDCAVSATGQGEYFIRAVVGYSICTGLKHKWRTMLDEAQASVPLDDDGNPTFMVHASEMWLEQSDVQAVADEV
MADVKDLGGTGGVIVVSPFGPAVFSFNTPGMYRGRATSAGVNEVAIYGDE
>Mature_370_residues
MNRIKHTLTAVSTAISLFFAAPLLAQDDYEPMEPRWSFAIHGGAGTIARENMTPEQDAEYRAALQQALDAGSDILKNGGS
AMDAVLAAIVLLEDDPKFNAGRGAVFTWEGENELDAALMDGKDRSAGAVTGVKAIKNPILLAREVMKDGRHVFLSGAGAE
EFAGERGLETAPPEWFATEARREALERLKARQLSSLDVDTKFGTVGAVALDLDGNMVAGTSTGGLTGKRWGRIGDVPVLG
AGTYADNRDCAVSATGQGEYFIRAVVGYSICTGLKHKWRTMLDEAQASVPLDDDGNPTFMVHASEMWLEQSDVQAVADEV
MADVKDLGGTGGVIVVSPFGPAVFSFNTPGMYRGRATSAGVNEVAIYGDE

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=223, Percent_Identity=48.8789237668161, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI145275200, Length=223, Percent_Identity=48.8789237668161, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI98991769, Length=283, Percent_Identity=32.8621908127208, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1787050, Length=325, Percent_Identity=44.3076923076923, Blast_Score=237, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI32564649, Length=198, Percent_Identity=30.3030303030303, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18921183, Length=340, Percent_Identity=36.4705882352941, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21357893, Length=271, Percent_Identity=32.8413284132841, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 39253; Mature: 39253

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00024 HEMOPEXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIKHTLTAVSTAISLFFAAPLLAQDDYEPMEPRWSFAIHGGAGTIARENMTPEQDAEY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHH
RAALQQALDAGSDILKNGGSAMDAVLAAIVLLEDDPKFNAGRGAVFTWEGENELDAALMD
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCCHHHHHCC
GKDRSAGAVTGVKAIKNPILLAREVMKDGRHVFLSGAGAEEFAGERGLETAPPEWFATEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHH
RREALERLKARQLSSLDVDTKFGTVGAVALDLDGNMVAGTSTGGLTGKRWGRIGDVPVLG
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEE
AGTYADNRDCAVSATGQGEYFIRAVVGYSICTGLKHKWRTMLDEAQASVPLDDDGNPTFM
CCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
VHASEMWLEQSDVQAVADEVMADVKDLGGTGGVIVVSPFGPAVFSFNTPGMYRGRATSAG
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCC
VNEVAIYGDE
CCEEEEEECC
>Mature Secondary Structure
MNRIKHTLTAVSTAISLFFAAPLLAQDDYEPMEPRWSFAIHGGAGTIARENMTPEQDAEY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHH
RAALQQALDAGSDILKNGGSAMDAVLAAIVLLEDDPKFNAGRGAVFTWEGENELDAALMD
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCCHHHHHCC
GKDRSAGAVTGVKAIKNPILLAREVMKDGRHVFLSGAGAEEFAGERGLETAPPEWFATEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHH
RREALERLKARQLSSLDVDTKFGTVGAVALDLDGNMVAGTSTGGLTGKRWGRIGDVPVLG
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEE
AGTYADNRDCAVSATGQGEYFIRAVVGYSICTGLKHKWRTMLDEAQASVPLDDDGNPTFM
CCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
VHASEMWLEQSDVQAVADEVMADVKDLGGTGGVIVVSPFGPAVFSFNTPGMYRGRATSAG
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCC
VNEVAIYGDE
CCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]