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The map label for this gene is metF [H]

Identifier: 85374846

GI number: 85374846

Start: 2049951

End: 2050889

Strand: Reverse

Name: metF [H]

Synonym: ELI_10095

Alternate gene names: 85374846

Gene position: 2050889-2049951 (Counterclockwise)

Preceding gene: 85374847

Following gene: 85374845

Centisome position: 67.19

GC content: 64.43

Gene sequence:

>939_bases
ATGACCGCCGCGCCGCCCCCGCTCGACCCGCAGCACGAAGCGCAAACGGCGCACGATAGCCCTCTGTTCTCCGGGCTGCC
CGGCGACATCGAAACGAGCTTCGAATTCTTCCCGCCGAAGACCGACAAGATGAGCGAAACGCTCTGGCGTAGCGTCGAGA
CGCTCAAGCCGCTCGGCCCGCGCTTCGCATCGGTGACCTATGGCGCTGGCGGCTCGACGCGCAGCCGCACACACAAACAG
GTCGTACGGATACAGAGCGAGGCGGGCATACCCGCTGCTGCGCATCTCACCTGCGTACAGGCAACGCGCGAGGAAGTAGA
CGAGGTCGCACGGCACTATTGGGACGAGGGCATTCGCCACATCGTTGCGCTGCGCGGCGATCCGCCCGACGGAGCGGCGC
ATTATACGCCGCATCCCGGCGGCTATGCCAATGCGGCCGATTTGATCACGGGATTGAAGAAGGTCGCCGATTTCGAGATT
TCCGTCGCCGCTTATCCGGAGGTGCATCCGGACTCGCCCGACCGGCAGGCCGATATCGCCAACCTCAAGACCAAGTTCGA
TGCCGGGGCGACCCGCGCGATCACGCAATTCTTCTTCGATCCGGAATGTTTCTTCCGCTTCCGCGACGAAGCGGCGGCGG
CAGGCATCCATGGCGAGATCGTGCCGGGGATTATGCCGGTGCTGAGCTTCGCGGCAGTCCAGCGTATGTCGACCCTGTGC
GGCACCGCCATTCCCGACTGGATGGCTGGCCTGTTCGACGGGCTCGATGATCGGCCCGAAGCGCGCCAGCTGGTCAGCGC
CACGATTGCCGCCGAATTGTGCCGCAGGCTCTATGCGGGCGGCGTGCGGCAATTCCACTTTTACACGCTCAACCGCGCCG
AATTGAGTTACGCGATCTGTCACATGCTCGGCCTGCGCCCCAAGGGAGCCACAGCATGA

Upstream 100 bases:

>100_bases
AGCCAATTGCGCTCGACGGCGATGAGTTGGTCGTGAAGGTCTGGCTTGGCACCCGTATCGCCAATCCTCACGTCACCCCC
TCGTACGAAAAGACACCCGC

Downstream 100 bases:

>100_bases
GCGCGCTGGCGAAACTCCGCGAGGCGGCGGCGGCGAACATCCTGATCAAGGATGGGCCCTATGGCACCGAGATCCAGCGC
GCGAAGCTCGCCGCAGAGGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTAAPPPLDPQHEAQTAHDSPLFSGLPGDIETSFEFFPPKTDKMSETLWRSVETLKPLGPRFASVTYGAGGSTRSRTHKQ
VVRIQSEAGIPAAAHLTCVQATREEVDEVARHYWDEGIRHIVALRGDPPDGAAHYTPHPGGYANAADLITGLKKVADFEI
SVAAYPEVHPDSPDRQADIANLKTKFDAGATRAITQFFFDPECFFRFRDEAAAAGIHGEIVPGIMPVLSFAAVQRMSTLC
GTAIPDWMAGLFDGLDDRPEARQLVSATIAAELCRRLYAGGVRQFHFYTLNRAELSYAICHMLGLRPKGATA

Sequences:

>Translated_312_residues
MTAAPPPLDPQHEAQTAHDSPLFSGLPGDIETSFEFFPPKTDKMSETLWRSVETLKPLGPRFASVTYGAGGSTRSRTHKQ
VVRIQSEAGIPAAAHLTCVQATREEVDEVARHYWDEGIRHIVALRGDPPDGAAHYTPHPGGYANAADLITGLKKVADFEI
SVAAYPEVHPDSPDRQADIANLKTKFDAGATRAITQFFFDPECFFRFRDEAAAAGIHGEIVPGIMPVLSFAAVQRMSTLC
GTAIPDWMAGLFDGLDDRPEARQLVSATIAAELCRRLYAGGVRQFHFYTLNRAELSYAICHMLGLRPKGATA
>Mature_311_residues
TAAPPPLDPQHEAQTAHDSPLFSGLPGDIETSFEFFPPKTDKMSETLWRSVETLKPLGPRFASVTYGAGGSTRSRTHKQV
VRIQSEAGIPAAAHLTCVQATREEVDEVARHYWDEGIRHIVALRGDPPDGAAHYTPHPGGYANAADLITGLKKVADFEIS
VAAYPEVHPDSPDRQADIANLKTKFDAGATRAITQFFFDPECFFRFRDEAAAAGIHGEIVPGIMPVLSFAAVQRMSTLCG
TAIPDWMAGLFDGLDDRPEARQLVSATIAAELCRRLYAGGVRQFHFYTLNRAELSYAICHMLGLRPKGATA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=32.280701754386, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1790377, Length=296, Percent_Identity=53.7162162162162, Blast_Score=329, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=30.9608540925267, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=281, Percent_Identity=30.9608540925267, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321313, Length=282, Percent_Identity=32.6241134751773, Blast_Score=152, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33975; Mature: 33843

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPPPLDPQHEAQTAHDSPLFSGLPGDIETSFEFFPPKTDKMSETLWRSVETLKPLGP
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RFASVTYGAGGSTRSRTHKQVVRIQSEAGIPAAAHLTCVQATREEVDEVARHYWDEGIRH
CHHEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IVALRGDPPDGAAHYTPHPGGYANAADLITGLKKVADFEISVAAYPEVHPDSPDRQADIA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
NLKTKFDAGATRAITQFFFDPECFFRFRDEAAAAGIHGEIVPGIMPVLSFAAVQRMSTLC
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GTAIPDWMAGLFDGLDDRPEARQLVSATIAAELCRRLYAGGVRQFHFYTLNRAELSYAIC
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHH
HMLGLRPKGATA
HHHCCCCCCCCC
>Mature Secondary Structure 
TAAPPPLDPQHEAQTAHDSPLFSGLPGDIETSFEFFPPKTDKMSETLWRSVETLKPLGP
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
RFASVTYGAGGSTRSRTHKQVVRIQSEAGIPAAAHLTCVQATREEVDEVARHYWDEGIRH
CHHEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IVALRGDPPDGAAHYTPHPGGYANAADLITGLKKVADFEISVAAYPEVHPDSPDRQADIA
EEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
NLKTKFDAGATRAITQFFFDPECFFRFRDEAAAAGIHGEIVPGIMPVLSFAAVQRMSTLC
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GTAIPDWMAGLFDGLDDRPEARQLVSATIAAELCRRLYAGGVRQFHFYTLNRAELSYAIC
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHH
HMLGLRPKGATA
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA