Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 85374845

GI number: 85374845

Start: 2048908

End: 2049954

Strand: Reverse

Name: metH [H]

Synonym: ELI_10090

Alternate gene names: 85374845

Gene position: 2049954-2048908 (Counterclockwise)

Preceding gene: 85374846

Following gene: 85374844

Centisome position: 67.16

GC content: 63.51

Gene sequence:

>1047_bases
ATGAGCGCGCTGGCGAAACTCCGCGAGGCGGCGGCGGCGAACATCCTGATCAAGGATGGGCCCTATGGCACCGAGATCCA
GCGCGCGAAGCTCGCCGCAGAGGACTATGCAGGCGACACCGGGCTGGTGCAGGACCAGAAGGGCAATAACGACCTCGTCA
ACCTCACCCAGCCGCAGGTGATCCGCACCATTTGCGACAGCTATATCGCGGCCGGGGCGACCGTGCTCGCCACCAACACC
TTCAACGCCAACCGGATCAGCCAGGCCGATTACGGCGCCGAAGGGCTGGTGCATGATATTAACGCGTCGGCTGCGCGGAT
CATTCGCGAGGCCTGCGACGATGCGACAGCGAAAGACGGCATCCCGCGCTTCGTATGCGGTGCCATGGGTCCGACCAACA
AGACGCTCTCGCTCAGCCCCGATGTCGAGGACCCCGGTTTCCGCGAAGTCACCTTCGATGAGATCGTCGACGTCTATCGC
GAGCAGGCGGCGGCCTTGCTGGAAGGCGGGGCCGAGTTCATCCTGATCGAAACGGTATTCGACACGCTCAACTGCAAGGC
CGCGATCATGGCGGTGAAGCAGCTCGAGCGCGAGCGCGGCGAGGAGATCCCGCTGATGATCTCGCTGACGCTGACGGACC
TGTCCGGGCGCAATCTGTCGGGTCATACCGTCGAGGCATTCTGGAACACGGTGCGGCATGCGAAGCCCGCCACGATCGGC
CTCAATTGCAGCTTCGGTGCCGAGCAATTGCGCCCGCATGTGCAGATCCTGTCGAACATCGCCGATACGCTGCTGATGGC
CTATCCCAACGCAGGCCTGCCCAACGATCTTGGCGAATATGACGAAATGCCCGAAACGACGGCCTCGCTGCTCAAGGTCT
GGGCCGAGAACGGGCGCGCGAACATTCTTGGTGGCTGCTGCGGTTCGACACCGGAACACATCGCGGCAATCGCCCAAACA
GTGAAGGGCGTCGAACCCCGGCAGGTCCCAGACGCGGTGTCGGAGATGCGACTGGCCGGGCTTGAGCCTTTCTCGGTGGC
GGCATGA

Upstream 100 bases:

>100_bases
CGGGCGGCGTGCGGCAATTCCACTTTTACACGCTCAACCGCGCCGAATTGAGTTACGCGATCTGTCACATGCTCGGCCTG
CGCCCCAAGGGAGCCACAGC

Downstream 100 bases:

>100_bases
CGGTTGGCATCGTTGACCAGGTCGCGATCCGCGAAGCATCGCCGGGCGATGCCGGTCGCCTGTCGCTGGTCGCCAGTGCG
ACTTTCCTCGAAACCTTCGC

Product: methionine synthase I cobalamin-binding subunit

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSALAKLREAAAANILIKDGPYGTEIQRAKLAAEDYAGDTGLVQDQKGNNDLVNLTQPQVIRTICDSYIAAGATVLATNT
FNANRISQADYGAEGLVHDINASAARIIREACDDATAKDGIPRFVCGAMGPTNKTLSLSPDVEDPGFREVTFDEIVDVYR
EQAAALLEGGAEFILIETVFDTLNCKAAIMAVKQLERERGEEIPLMISLTLTDLSGRNLSGHTVEAFWNTVRHAKPATIG
LNCSFGAEQLRPHVQILSNIADTLLMAYPNAGLPNDLGEYDEMPETTASLLKVWAENGRANILGGCCGSTPEHIAAIAQT
VKGVEPRQVPDAVSEMRLAGLEPFSVAA

Sequences:

>Translated_348_residues
MSALAKLREAAAANILIKDGPYGTEIQRAKLAAEDYAGDTGLVQDQKGNNDLVNLTQPQVIRTICDSYIAAGATVLATNT
FNANRISQADYGAEGLVHDINASAARIIREACDDATAKDGIPRFVCGAMGPTNKTLSLSPDVEDPGFREVTFDEIVDVYR
EQAAALLEGGAEFILIETVFDTLNCKAAIMAVKQLERERGEEIPLMISLTLTDLSGRNLSGHTVEAFWNTVRHAKPATIG
LNCSFGAEQLRPHVQILSNIADTLLMAYPNAGLPNDLGEYDEMPETTASLLKVWAENGRANILGGCCGSTPEHIAAIAQT
VKGVEPRQVPDAVSEMRLAGLEPFSVAA
>Mature_347_residues
SALAKLREAAAANILIKDGPYGTEIQRAKLAAEDYAGDTGLVQDQKGNNDLVNLTQPQVIRTICDSYIAAGATVLATNTF
NANRISQADYGAEGLVHDINASAARIIREACDDATAKDGIPRFVCGAMGPTNKTLSLSPDVEDPGFREVTFDEIVDVYRE
QAAALLEGGAEFILIETVFDTLNCKAAIMAVKQLERERGEEIPLMISLTLTDLSGRNLSGHTVEAFWNTVRHAKPATIGL
NCSFGAEQLRPHVQILSNIADTLLMAYPNAGLPNDLGEYDEMPETTASLLKVWAENGRANILGGCCGSTPEHIAAIAQTV
KGVEPRQVPDAVSEMRLAGLEPFSVAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=339, Percent_Identity=48.6725663716814, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1790450, Length=348, Percent_Identity=50.2873563218391, Blast_Score=338, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17535405, Length=347, Percent_Identity=47.8386167146974, Blast_Score=335, Evalue=2e-92,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37170; Mature: 37039

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALAKLREAAAANILIKDGPYGTEIQRAKLAAEDYAGDTGLVQDQKGNNDLVNLTQPQV
CCHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCEECCCHHH
IRTICDSYIAAGATVLATNTFNANRISQADYGAEGLVHDINASAARIIREACDDATAKDG
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHCC
IPRFVCGAMGPTNKTLSLSPDVEDPGFREVTFDEIVDVYREQAAALLEGGAEFILIETVF
CCHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
DTLNCKAAIMAVKQLERERGEEIPLMISLTLTDLSGRNLSGHTVEAFWNTVRHAKPATIG
HHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
LNCSFGAEQLRPHVQILSNIADTLLMAYPNAGLPNDLGEYDEMPETTASLLKVWAENGRA
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
NILGGCCGSTPEHIAAIAQTVKGVEPRQVPDAVSEMRLAGLEPFSVAA
EEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SALAKLREAAAANILIKDGPYGTEIQRAKLAAEDYAGDTGLVQDQKGNNDLVNLTQPQV
CHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCEECCCHHH
IRTICDSYIAAGATVLATNTFNANRISQADYGAEGLVHDINASAARIIREACDDATAKDG
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCHHHCC
IPRFVCGAMGPTNKTLSLSPDVEDPGFREVTFDEIVDVYREQAAALLEGGAEFILIETVF
CCHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
DTLNCKAAIMAVKQLERERGEEIPLMISLTLTDLSGRNLSGHTVEAFWNTVRHAKPATIG
HHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
LNCSFGAEQLRPHVQILSNIADTLLMAYPNAGLPNDLGEYDEMPETTASLLKVWAENGRA
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
NILGGCCGSTPEHIAAIAQTVKGVEPRQVPDAVSEMRLAGLEPFSVAA
EEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]