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The map label for this gene is fadE12 [H]

Identifier: 85374747

GI number: 85374747

Start: 1955274

End: 1956413

Strand: Reverse

Name: fadE12 [H]

Synonym: ELI_09600

Alternate gene names: 85374747

Gene position: 1956413-1955274 (Counterclockwise)

Preceding gene: 85374748

Following gene: 85374746

Centisome position: 64.09

GC content: 64.47

Gene sequence:

>1140_bases
ATGCCACTTTATCACGACGACGATCAGGCCATGCTGGCTGAAACCGCCAGCCAGTTCATGGGCGAGGAAGGCGCGATCGC
GAAACAGCTCCGCCACTGGCGCGACCGCGATTGCAAGGACGGCTTCGGCCACGGGCTGTGGGAGCAGTTCGGCGAGATGG
GTTTCACCGGCATCCTCGTCGGCGAAGACGATGGCGGGCTCGGCATGGGCCATGTCGAAGCCGGGATCGTGCTTGAGGAG
ATCGGTCGCAATCTCACCCCGTCGCCTTTCCTGACCTCGTCGGTACTCGCCGCAACGGCGCTCAATCACGGCAGCGACGA
TGTGAAGGGCCGCTATCTACCCGGCCTCATCGAAGGCAAGAGCGTGTTCGCCGTCGCCATCGACGAAGGTGCCAAGCACC
GGCCCGAAACAATCGCCTGCAAGGCCGAGCGCTCAGGCAATGGCTTCAAGCTTACGGGCAAGAAGGATTTCGTGGTCCAC
GGCGCGAGCGCCGATATGCTGGTGGTCGCCGCGCGCACTTCCGGCAGCGACAGCGACGAGGATGGTCTCACGCTCTTCGC
CGTGCCCAAGGATGCGGCCAATATGAGCCATGACGCTGTTCGCCTGGTCGACAGCTCGATCGCAACGCACACCAGCTTCG
ACGGGGTCGAGCTCGATGGCGATGCGGTGATCGGCGAGGTCGACAATGGCCGCTCGATCCTCAACGCCATGCTCGATGCG
GGCCGCGTCGGCAGCGCCGCCGAAGGTGTCGGCGTTGCGCGCGGGGCGATGGACATGACCGTCGATTACCTCAAGCAGCG
CAAGCAGTTCGGCAAGCATATCGGCGAGTTCCAGGCGCTCCAGCATCGCGCCGCGCACCTCTATTCCGAAGTCGAGATCG
CCCGCGCCGCCGTGATCAAGGCGCAGCAGCTGCTCGATGGCGGCAGCGAGCGGGCCAGCCTGATGGCCTCTGTCGCCAAG
GCCAAGGTCGCCAAGACAGCCGGGCTCGCGGTGCGCGAAGGCGTGCAGATGCATGGCGGCATCGGCATGACAGACGAATA
CGATATCGGCCTCTACATGAAGCGCGACAGGGCCTTGCAGGAGTTCCTCGGTGACGCCTACTACCACGCCAACCGCGTGG
CTGAGTTGTCGGGGTATTGA

Upstream 100 bases:

>100_bases
GCGCGTCACCCCAGCGAAAGCTGGGGTCTCGTGCCGCTTGGTCGGGTTAGTAACGGCCAGATCCCAGCTTGCGCTGGGAT
GACGACATAGGGACAGGACA

Downstream 100 bases:

>100_bases
CCAGCGCGTCGCCCCAGCGAAGGCTGGGGCCTCTGTCGGCTTGGTCCGACCAGGATTGACCAGATCCCAGCTTTCGCTGG
GATGACGGGAGAAGAAGCAT

Product: putative acyl-CoA dehydrogenase

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPLYHDDDQAMLAETASQFMGEEGAIAKQLRHWRDRDCKDGFGHGLWEQFGEMGFTGILVGEDDGGLGMGHVEAGIVLEE
IGRNLTPSPFLTSSVLAATALNHGSDDVKGRYLPGLIEGKSVFAVAIDEGAKHRPETIACKAERSGNGFKLTGKKDFVVH
GASADMLVVAARTSGSDSDEDGLTLFAVPKDAANMSHDAVRLVDSSIATHTSFDGVELDGDAVIGEVDNGRSILNAMLDA
GRVGSAAEGVGVARGAMDMTVDYLKQRKQFGKHIGEFQALQHRAAHLYSEVEIARAAVIKAQQLLDGGSERASLMASVAK
AKVAKTAGLAVREGVQMHGGIGMTDEYDIGLYMKRDRALQEFLGDAYYHANRVAELSGY

Sequences:

>Translated_379_residues
MPLYHDDDQAMLAETASQFMGEEGAIAKQLRHWRDRDCKDGFGHGLWEQFGEMGFTGILVGEDDGGLGMGHVEAGIVLEE
IGRNLTPSPFLTSSVLAATALNHGSDDVKGRYLPGLIEGKSVFAVAIDEGAKHRPETIACKAERSGNGFKLTGKKDFVVH
GASADMLVVAARTSGSDSDEDGLTLFAVPKDAANMSHDAVRLVDSSIATHTSFDGVELDGDAVIGEVDNGRSILNAMLDA
GRVGSAAEGVGVARGAMDMTVDYLKQRKQFGKHIGEFQALQHRAAHLYSEVEIARAAVIKAQQLLDGGSERASLMASVAK
AKVAKTAGLAVREGVQMHGGIGMTDEYDIGLYMKRDRALQEFLGDAYYHANRVAELSGY
>Mature_378_residues
PLYHDDDQAMLAETASQFMGEEGAIAKQLRHWRDRDCKDGFGHGLWEQFGEMGFTGILVGEDDGGLGMGHVEAGIVLEEI
GRNLTPSPFLTSSVLAATALNHGSDDVKGRYLPGLIEGKSVFAVAIDEGAKHRPETIACKAERSGNGFKLTGKKDFVVHG
ASADMLVVAARTSGSDSDEDGLTLFAVPKDAANMSHDAVRLVDSSIATHTSFDGVELDGDAVIGEVDNGRSILNAMLDAG
RVGSAAEGVGVARGAMDMTVDYLKQRKQFGKHIGEFQALQHRAAHLYSEVEIARAAVIKAQQLLDGGSERASLMASVAKA
KVAKTAGLAVREGVQMHGGIGMTDEYDIGLYMKRDRALQEFLGDAYYHANRVAELSGY

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=378, Percent_Identity=26.4550264550265, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI226958412, Length=366, Percent_Identity=28.4153005464481, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI226958414, Length=326, Percent_Identity=29.1411042944785, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4501857, Length=314, Percent_Identity=27.7070063694268, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI21361497, Length=333, Percent_Identity=25.2252252252252, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI4557233, Length=347, Percent_Identity=26.2247838616715, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI187960098, Length=326, Percent_Identity=27.6073619631902, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI76496475, Length=366, Percent_Identity=24.8633879781421, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI4557235, Length=366, Percent_Identity=24.8633879781421, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI4557231, Length=326, Percent_Identity=27.6073619631902, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI7656849, Length=361, Percent_Identity=23.2686980609418, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4503943, Length=342, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI7669494, Length=321, Percent_Identity=23.98753894081, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786223, Length=365, Percent_Identity=26.027397260274, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081958, Length=334, Percent_Identity=23.6526946107784, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=336, Percent_Identity=29.7619047619048, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17508101, Length=378, Percent_Identity=26.7195767195767, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538396, Length=368, Percent_Identity=27.9891304347826, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71990804, Length=264, Percent_Identity=29.5454545454545, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17533517, Length=316, Percent_Identity=25, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71985184, Length=375, Percent_Identity=24.5333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17569725, Length=341, Percent_Identity=26.099706744868, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=232, Percent_Identity=29.7413793103448, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17570075, Length=332, Percent_Identity=26.2048192771084, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534899, Length=342, Percent_Identity=24.5614035087719, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534353, Length=344, Percent_Identity=22.9651162790698, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71985192, Length=334, Percent_Identity=24.251497005988, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551932, Length=329, Percent_Identity=24.9240121580547, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17505929, Length=328, Percent_Identity=25.609756097561, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982178, Length=291, Percent_Identity=24.7422680412371, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562446, Length=336, Percent_Identity=23.2142857142857, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355753, Length=370, Percent_Identity=30, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24646207, Length=367, Percent_Identity=24.7956403269755, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24666513, Length=365, Percent_Identity=25.7534246575342, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21356377, Length=302, Percent_Identity=28.1456953642384, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24660351, Length=372, Percent_Identity=27.6881720430108, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281363737, Length=316, Percent_Identity=24.0506329113924, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 40400; Mature: 40269

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYHDDDQAMLAETASQFMGEEGAIAKQLRHWRDRDCKDGFGHGLWEQFGEMGFTGILV
CCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEE
GEDDGGLGMGHVEAGIVLEEIGRNLTPSPFLTSSVLAATALNHGSDDVKGRYLPGLIEGK
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCC
SVFAVAIDEGAKHRPETIACKAERSGNGFKLTGKKDFVVHGASADMLVVAARTSGSDSDE
EEEEEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCC
DGLTLFAVPKDAANMSHDAVRLVDSSIATHTSFDGVELDGDAVIGEVDNGRSILNAMLDA
CCEEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHC
GRVGSAAEGVGVARGAMDMTVDYLKQRKQFGKHIGEFQALQHRAAHLYSEVEIARAAVIK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQLLDGGSERASLMASVAKAKVAKTAGLAVREGVQMHGGIGMTDEYDIGLYMKRDRALQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEHHHHHHH
EFLGDAYYHANRVAELSGY
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLYHDDDQAMLAETASQFMGEEGAIAKQLRHWRDRDCKDGFGHGLWEQFGEMGFTGILV
CCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEE
GEDDGGLGMGHVEAGIVLEEIGRNLTPSPFLTSSVLAATALNHGSDDVKGRYLPGLIEGK
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCC
SVFAVAIDEGAKHRPETIACKAERSGNGFKLTGKKDFVVHGASADMLVVAARTSGSDSDE
EEEEEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCC
DGLTLFAVPKDAANMSHDAVRLVDSSIATHTSFDGVELDGDAVIGEVDNGRSILNAMLDA
CCEEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHC
GRVGSAAEGVGVARGAMDMTVDYLKQRKQFGKHIGEFQALQHRAAHLYSEVEIARAAVIK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQLLDGGSERASLMASVAKAKVAKTAGLAVREGVQMHGGIGMTDEYDIGLYMKRDRALQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEHHHHHHH
EFLGDAYYHANRVAELSGY
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]