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The map label for this gene is yngJ [H]

Identifier: 85374674

GI number: 85374674

Start: 1876618

End: 1877709

Strand: Reverse

Name: yngJ [H]

Synonym: ELI_09235

Alternate gene names: 85374674

Gene position: 1877709-1876618 (Counterclockwise)

Preceding gene: 85374675

Following gene: 85374673

Centisome position: 61.52

GC content: 63.83

Gene sequence:

>1092_bases
ATGGATTTCAGCTTCACCGAAGAGCAGGAGATGCTGCGCGACGGCGTCGGCAAGTATCTCGAGAAGAACTACGATTTCGA
AACGCGGCAGGCGCTGGTGCGCTCCGAAGAGCCATGGTCGCAAAAGGCTTGGCAGCAGTTTGCGGAGTTCGGCCTGCTGG
CGCTGCCCTTTGCCGAGGAGCAGGGCGGACTCGGCGGCTCGATCAGCGATTGTGTCGCTGTGGCCGAGCTGTTCGGCAAA
CATCTCGTCATCGAACCCTACGCAGCTTCGATCATGCTGGCCGGGGCGGCGTTGGCTGCGAGCGACGATGGCCCGGCGCA
AGACTGGCTGGAGAAAGTGATTGCAGGCGAGGCCATCGCCGCCTTCGCCTATGAGGAAGGGGCGGGTACTGCGGCGCCGG
ACATGGTTGCCATGATCGCTGAACCGGTCGGCGACGCATTCACGCTGACAGGCGAGAAACGCCTGGTCGTGGCCGGTGGC
GAAGCCGACTGCCTAATCGTCGTGGCGCGTCGGGGTGAGGACGGACCGCTCGGCGTTTTCCTTGTCGAGAAGACAGCGCA
ACGCATGGAAGCACGCAGCTACACGACGATCGACGGGCGAAGCGCCGCTAATATCCGCTTCGACGCGACACCTGCGACCC
TGCTCGACGGCGCCGAAGCCGCCCTCGACCGCATCCTTGCCCATGCCATCATCGTGCAATCGGCAGAGGCGGTCGGCGCG
ATGGGCGCACTGCTTGCGCTTACCAGCGATTACGCCATGACCCGCAAGCAGTTCGGTGTGCCCATCGGGACCTTCCAGGC
CGTGGCGCACCGGCTTGCGGACATGAAGATCGCTTACACCAAGGCGCGGGCGACGCTACTTTACACCACGGCAATGGCGG
AATCCGGCGCTGTAAAACCGCGCGATATCGCCATCCTCAAGGGGCAGGTCGGCAAGCTCGGCAAGGCGGTTGGCGAAGCA
GCGATCCAGACCCATGGCGGGGTCGGCATGACCGACGAGCTGAGCGTGAGCCACTATCACAAGCGACTGCTCGCCTGCGA
TGCGATGTTCGGCAGCCATGACTATCACTTGCGGGTGGTCGGACAGGGCTGA

Upstream 100 bases:

>100_bases
CATGCCGCTCTATCTCAACAGCCGCGCGACGACGATCTACGCAGGCTCCAACGAAGTGCAGCGCGACCTGATTGCCCGCA
CAGTGGCGGGGAGCTGAACG

Downstream 100 bases:

>100_bases
GCCATGCGGGTGCTCGGAGCGATCCTGGCGGGCGGCCGTTCGCGACGCTTCGGCAGCGACAAGGCGCACGCAACCATCGA
CGGCGAGCGGCTGATCGATC

Product: acyl-CoA dehydrogenase family protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDFSFTEEQEMLRDGVGKYLEKNYDFETRQALVRSEEPWSQKAWQQFAEFGLLALPFAEEQGGLGGSISDCVAVAELFGK
HLVIEPYAASIMLAGAALAASDDGPAQDWLEKVIAGEAIAAFAYEEGAGTAAPDMVAMIAEPVGDAFTLTGEKRLVVAGG
EADCLIVVARRGEDGPLGVFLVEKTAQRMEARSYTTIDGRSAANIRFDATPATLLDGAEAALDRILAHAIIVQSAEAVGA
MGALLALTSDYAMTRKQFGVPIGTFQAVAHRLADMKIAYTKARATLLYTTAMAESGAVKPRDIAILKGQVGKLGKAVGEA
AIQTHGGVGMTDELSVSHYHKRLLACDAMFGSHDYHLRVVGQG

Sequences:

>Translated_363_residues
MDFSFTEEQEMLRDGVGKYLEKNYDFETRQALVRSEEPWSQKAWQQFAEFGLLALPFAEEQGGLGGSISDCVAVAELFGK
HLVIEPYAASIMLAGAALAASDDGPAQDWLEKVIAGEAIAAFAYEEGAGTAAPDMVAMIAEPVGDAFTLTGEKRLVVAGG
EADCLIVVARRGEDGPLGVFLVEKTAQRMEARSYTTIDGRSAANIRFDATPATLLDGAEAALDRILAHAIIVQSAEAVGA
MGALLALTSDYAMTRKQFGVPIGTFQAVAHRLADMKIAYTKARATLLYTTAMAESGAVKPRDIAILKGQVGKLGKAVGEA
AIQTHGGVGMTDELSVSHYHKRLLACDAMFGSHDYHLRVVGQG
>Mature_363_residues
MDFSFTEEQEMLRDGVGKYLEKNYDFETRQALVRSEEPWSQKAWQQFAEFGLLALPFAEEQGGLGGSISDCVAVAELFGK
HLVIEPYAASIMLAGAALAASDDGPAQDWLEKVIAGEAIAAFAYEEGAGTAAPDMVAMIAEPVGDAFTLTGEKRLVVAGG
EADCLIVVARRGEDGPLGVFLVEKTAQRMEARSYTTIDGRSAANIRFDATPATLLDGAEAALDRILAHAIIVQSAEAVGA
MGALLALTSDYAMTRKQFGVPIGTFQAVAHRLADMKIAYTKARATLLYTTAMAESGAVKPRDIAILKGQVGKLGKAVGEA
AIQTHGGVGMTDELSVSHYHKRLLACDAMFGSHDYHLRVVGQG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4503943, Length=273, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI226958412, Length=361, Percent_Identity=24.0997229916898, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226958414, Length=329, Percent_Identity=24.6200607902736, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI7669494, Length=258, Percent_Identity=28.2945736434109, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557235, Length=379, Percent_Identity=24.2744063324538, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI76496475, Length=379, Percent_Identity=24.2744063324538, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4557233, Length=310, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786223, Length=385, Percent_Identity=22.0779220779221, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538396, Length=356, Percent_Identity=26.123595505618, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17506239, Length=380, Percent_Identity=23.1578947368421, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508101, Length=313, Percent_Identity=23.3226837060703, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355753, Length=314, Percent_Identity=29.2993630573248, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356377, Length=354, Percent_Identity=24.2937853107345, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24660351, Length=353, Percent_Identity=23.7960339943343, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 38534; Mature: 38534

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSFTEEQEMLRDGVGKYLEKNYDFETRQALVRSEEPWSQKAWQQFAEFGLLALPFAEE
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCC
QGGLGGSISDCVAVAELFGKHLVIEPYAASIMLAGAALAASDDGPAQDWLEKVIAGEAIA
CCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCHHH
AFAYEEGAGTAAPDMVAMIAEPVGDAFTLTGEKRLVVAGGEADCLIVVARRGEDGPLGVF
EEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEE
LVEKTAQRMEARSYTTIDGRSAANIRFDATPATLLDGAEAALDRILAHAIIVQSAEAVGA
EEHHHHHHHHHCCCEEECCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MGALLALTSDYAMTRKQFGVPIGTFQAVAHRLADMKIAYTKARATLLYTTAMAESGAVKP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEHHHEEEEEEEHHCCCCCCCC
RDIAILKGQVGKLGKAVGEAAIQTHGGVGMTDELSVSHYHKRLLACDAMFGSHDYHLRVV
CCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GQG
ECC
>Mature Secondary Structure
MDFSFTEEQEMLRDGVGKYLEKNYDFETRQALVRSEEPWSQKAWQQFAEFGLLALPFAEE
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCC
QGGLGGSISDCVAVAELFGKHLVIEPYAASIMLAGAALAASDDGPAQDWLEKVIAGEAIA
CCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCHHH
AFAYEEGAGTAAPDMVAMIAEPVGDAFTLTGEKRLVVAGGEADCLIVVARRGEDGPLGVF
EEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEE
LVEKTAQRMEARSYTTIDGRSAANIRFDATPATLLDGAEAALDRILAHAIIVQSAEAVGA
EEHHHHHHHHHCCCEEECCCCCCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MGALLALTSDYAMTRKQFGVPIGTFQAVAHRLADMKIAYTKARATLLYTTAMAESGAVKP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEHHHEEEEEEEHHCCCCCCCC
RDIAILKGQVGKLGKAVGEAAIQTHGGVGMTDELSVSHYHKRLLACDAMFGSHDYHLRVV
CCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GQG
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]