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The map label for this gene is hemC

Identifier: 85374658

GI number: 85374658

Start: 1862010

End: 1862888

Strand: Reverse

Name: hemC

Synonym: ELI_09155

Alternate gene names: 85374658

Gene position: 1862888-1862010 (Counterclockwise)

Preceding gene: 85374672

Following gene: 85374657

Centisome position: 61.03

GC content: 68.03

Gene sequence:

>879_bases
ATGGCGCAGGCGGTGGAGACCCGCAATCGCCTCTGCGCGGCGCACGGCTGGGACGAAGAGGCGGTCGAATTGGTCCCGGT
GGTGGCGAGTGGCGACAAGATTCAGGATCGCCCGCTGGCCGAGATCGGCGGCAAGGCGCTGTGGACCAAGGAACTCGACG
CCTGGCTGGGCGAGGGCCGCATTGATGCGGCGGTCCATTCGATGAAGGATGTCGAGACGTTCCGGCCCGAGGCCCTGCAT
ATCGCCGCCATCCTGCCACGCGAGGACAAGCGCGATGTGCTGGTCGGCGCGGCCGGGATCGAAGACATTCCAAAGGGCGC
GAAAGTGGGCACCAGCGCGCCGCGTCGGGCGGCGCAGATGCTCAGTCTCAGACCCGATGTAGAGGTCGTGACTTTCCGCG
GCAATGTCGCCACCCGGCTCGGCAAGCTGGAGGCGGGGGAGGCGGACGTGACCTTGCTCGCTGCCGCCGGGCTCAAGCGG
CTCGGCGAACTGGACATCGGTAGCCCGCTCGAGCTCGACGAATGGCTACCGGCTGCGGCGCAGGGGGCGATCGGCATCGA
GTGTCGCGCGGACGATGCCGGGGCCCGCGCGCTTCTTTCGCCGATCGATCACGCGCCATCGCGCGCCGAGATCATGGCCG
AACGCGCACTGCTCGCAGGACTGAACGGGACCTGCCACAGCCCGATCGCCGTTCTCGCTGAGGCGATGGGAAATCGGCTG
TCGATCCGCGCTACGCTCTACAGTCCGGATGGAACGGACAAGGTAGCGGGTGAAGGCGAACTCGCACTGGCCGACTACGC
TGCGATCGAGCGGCTTGCTGCCGACTTGCTCGATCGCGCCAGCCCGGCGATCCGCACCGTATTCGGCGGGGCCGCATGA

Upstream 100 bases:

>100_bases
TCATGCGGCTTCCCCTTAGACGAAGCTCTCGCTAGGGCAAGCGAACCGCATGAGTGAGACGCCCATCCTCCGCCTCGGAA
CCCGCACTTCTCCGCTCGCC

Downstream 100 bases:

>100_bases
GCCGACCCGTCTTTGTCCTGCGCCCGGAGCCCGGTCTCACCACCACTCTCGTGGCCGCTTTCGAGAGAGGCCTGAACGCG
CGCGGGATGCCGCTGAGTAC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAQAVETRNRLCAAHGWDEEAVELVPVVASGDKIQDRPLAEIGGKALWTKELDAWLGEGRIDAAVHSMKDVETFRPEALH
IAAILPREDKRDVLVGAAGIEDIPKGAKVGTSAPRRAAQMLSLRPDVEVVTFRGNVATRLGKLEAGEADVTLLAAAGLKR
LGELDIGSPLELDEWLPAAAQGAIGIECRADDAGARALLSPIDHAPSRAEIMAERALLAGLNGTCHSPIAVLAEAMGNRL
SIRATLYSPDGTDKVAGEGELALADYAAIERLAADLLDRASPAIRTVFGGAA

Sequences:

>Translated_292_residues
MAQAVETRNRLCAAHGWDEEAVELVPVVASGDKIQDRPLAEIGGKALWTKELDAWLGEGRIDAAVHSMKDVETFRPEALH
IAAILPREDKRDVLVGAAGIEDIPKGAKVGTSAPRRAAQMLSLRPDVEVVTFRGNVATRLGKLEAGEADVTLLAAAGLKR
LGELDIGSPLELDEWLPAAAQGAIGIECRADDAGARALLSPIDHAPSRAEIMAERALLAGLNGTCHSPIAVLAEAMGNRL
SIRATLYSPDGTDKVAGEGELALADYAAIERLAADLLDRASPAIRTVFGGAA
>Mature_291_residues
AQAVETRNRLCAAHGWDEEAVELVPVVASGDKIQDRPLAEIGGKALWTKELDAWLGEGRIDAAVHSMKDVETFRPEALHI
AAILPREDKRDVLVGAAGIEDIPKGAKVGTSAPRRAAQMLSLRPDVEVVTFRGNVATRLGKLEAGEADVTLLAAAGLKRL
GELDIGSPLELDEWLPAAAQGAIGIECRADDAGARALLSPIDHAPSRAEIMAERALLAGLNGTCHSPIAVLAEAMGNRLS
IRATLYSPDGTDKVAGEGELALADYAAIERLAADLLDRASPAIRTVFGGAA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI20149500, Length=241, Percent_Identity=37.344398340249, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI66933009, Length=241, Percent_Identity=37.344398340249, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI48994974, Length=259, Percent_Identity=45.1737451737452, Blast_Score=198, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319996, Length=249, Percent_Identity=36.144578313253, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20130425, Length=233, Percent_Identity=37.3390557939914, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 30635; Mature: 30504

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAVETRNRLCAAHGWDEEAVELVPVVASGDKIQDRPLAEIGGKALWTKELDAWLGEGR
CCCHHHHHHHEECCCCCCCCHHEEEEEEECCCCCCCCCHHHHCCCEEEHHHHHHHHCCCC
IDAAVHSMKDVETFRPEALHIAAILPREDKRDVLVGAAGIEDIPKGAKVGTSAPRRAAQM
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
LSLRPDVEVVTFRGNVATRLGKLEAGEADVTLLAAAGLKRLGELDIGSPLELDEWLPAAA
HHCCCCCEEEEECCCHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHCCHHH
QGAIGIECRADDAGARALLSPIDHAPSRAEIMAERALLAGLNGTCHSPIAVLAEAMGNRL
CCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEE
SIRATLYSPDGTDKVAGEGELALADYAAIERLAADLLDRASPAIRTVFGGAA
EEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
AQAVETRNRLCAAHGWDEEAVELVPVVASGDKIQDRPLAEIGGKALWTKELDAWLGEGR
CCHHHHHHHEECCCCCCCCHHEEEEEEECCCCCCCCCHHHHCCCEEEHHHHHHHHCCCC
IDAAVHSMKDVETFRPEALHIAAILPREDKRDVLVGAAGIEDIPKGAKVGTSAPRRAAQM
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
LSLRPDVEVVTFRGNVATRLGKLEAGEADVTLLAAAGLKRLGELDIGSPLELDEWLPAAA
HHCCCCCEEEEECCCHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHCCHHH
QGAIGIECRADDAGARALLSPIDHAPSRAEIMAERALLAGLNGTCHSPIAVLAEAMGNRL
CCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEE
SIRATLYSPDGTDKVAGEGELALADYAAIERLAADLLDRASPAIRTVFGGAA
EEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA