Click here to switch to the map view.

The map label for this gene is mscL [H]

Identifier: 85374614

GI number: 85374614

Start: 1820838

End: 1821347

Strand: Reverse

Name: mscL [H]

Synonym: ELI_08935

Alternate gene names: 85374614

Gene position: 1821347-1820838 (Counterclockwise)

Preceding gene: 85374621

Following gene: 85374609

Centisome position: 59.67

GC content: 61.76

Gene sequence:

>510_bases
ATGAGCCTTGGGACGGAATTCAAGAAATTCATCGCGCGCGGCAACGTCGTCGATCTCGCCGTCGGCGTGGTGATCGGCGC
GGCGTTCAGCGGCATCGTCACCCAGCTGACCGAAGCGATCCTCATGCCGCTGATCGGCTGGATATTCGGCGATATCGACT
TTTCCAACTGGTTCCTGCGGCTGGGCGAGATCCCCGAAGGCTATGAAGGCGCGCTCGACAATTACGAGCAGTTGAAAGAG
GCCGGTGTCGCGATGATCGGCTACGGCGCGCTGATCACGGCGCTGATCAACTTCCTGATCGTGGCCTTTGCCTTGTTCAT
GCTCGTGCGCAGCGTCAACAAGGTCAGCGACGAGATCAAGGCGCGGCAGGCCGAGACCGAGGACAGCTCCAAGAGCGACG
ACGTGCCGACCGACCCGCAGCTCGATGTGCTCAAGAAGATCCTCGCCGAGCTGCGTTCGGATGGGGCGAGCAAGCCGGAT
TCGACGCCGGAAAAGGGGCCGATGGGCTAG

Upstream 100 bases:

>100_bases
CTATATAGGACAGTCGCTGCCCGGTTCAAGCCGCAAGCTGCTCTCTTGGCAAGCTTTAGGCGACATGACACAGCAGCGCT
ACACCATTGGAGGAGCTATC

Downstream 100 bases:

>100_bases
CAGCCGGTCTCGCTCACTTCACATTAAGGCGTTTTCGCTTATATGGGACCTGTCCGTTTCGGCGGGCTATGGTGATAAAC
TGCGGTGTGTAATAGACGCA

Product: mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSLGTEFKKFIARGNVVDLAVGVVIGAAFSGIVTQLTEAILMPLIGWIFGDIDFSNWFLRLGEIPEGYEGALDNYEQLKE
AGVAMIGYGALITALINFLIVAFALFMLVRSVNKVSDEIKARQAETEDSSKSDDVPTDPQLDVLKKILAELRSDGASKPD
STPEKGPMG

Sequences:

>Translated_169_residues
MSLGTEFKKFIARGNVVDLAVGVVIGAAFSGIVTQLTEAILMPLIGWIFGDIDFSNWFLRLGEIPEGYEGALDNYEQLKE
AGVAMIGYGALITALINFLIVAFALFMLVRSVNKVSDEIKARQAETEDSSKSDDVPTDPQLDVLKKILAELRSDGASKPD
STPEKGPMG
>Mature_168_residues
SLGTEFKKFIARGNVVDLAVGVVIGAAFSGIVTQLTEAILMPLIGWIFGDIDFSNWFLRLGEIPEGYEGALDNYEQLKEA
GVAMIGYGALITALINFLIVAFALFMLVRSVNKVSDEIKARQAETEDSSKSDDVPTDPQLDVLKKILAELRSDGASKPDS
TPEKGPMG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=150, Percent_Identity=38, Blast_Score=87, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 18242; Mature: 18111

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGTEFKKFIARGNVVDLAVGVVIGAAFSGIVTQLTEAILMPLIGWIFGDIDFSNWFLR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LGEIPEGYEGALDNYEQLKEAGVAMIGYGALITALINFLIVAFALFMLVRSVNKVSDEIK
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQAETEDSSKSDDVPTDPQLDVLKKILAELRSDGASKPDSTPEKGPMG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLGTEFKKFIARGNVVDLAVGVVIGAAFSGIVTQLTEAILMPLIGWIFGDIDFSNWFLR
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LGEIPEGYEGALDNYEQLKEAGVAMIGYGALITALINFLIVAFALFMLVRSVNKVSDEIK
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQAETEDSSKSDDVPTDPQLDVLKKILAELRSDGASKPDSTPEKGPMG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA