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The map label for this gene is ksgA

Identifier: 85374607

GI number: 85374607

Start: 1812482

End: 1813327

Strand: Direct

Name: ksgA

Synonym: ELI_08900

Alternate gene names: 85374607

Gene position: 1812482-1813327 (Clockwise)

Preceding gene: 85374606

Following gene: 85374610

Centisome position: 59.38

GC content: 66.19

Gene sequence:

>846_bases
ATGCCTGAGCTTCCGCCGCTGCGCGACGTCATCGCGAAACATGGCCTCTCCGCCTCGAAGGCGCTGGGGCAGAACTTCCT
GTTCGATGCGCAATTGCTCGACCGCATCGCCGGAATCCCGGGTGGTCTCGAAAACCGGGCCGTGCTCGAAATCGGGCCTG
GTCCCGGCGGGCTCACCCGCGCTTTGCTAAAGGCCGGCGCGCGCGTGACGGCGATCGAGATGGACCGCCGCTGCCTGCCT
GCGCTTGCCGAGCTGTCGGAGGTTTACCCCGGCAAACTATCCGTCATCCATGGCGATGCGATGAAGCTCGATCATGCCGA
ATTGATGGGCGAGCCATTCGCTGTCGTCGCCAACCTGCCCTACAATGTCGGCACCGCTCTCTTCGTGCGCTGGCTCGGCG
GCGAGACTTGGCCGCCGCAATGGACGTCGCTCACCTTGATGTTCCAGCAGGAAGTCGCCCAGCGGATCGTCTCCACACCG
GGCACATCGGCGTACGGCCGCCTCGCCGTGCTGGCGCAATGGCGCAGCGCCGCCAGCATGCCGATGAAGGTTCATCGCAG
CGCCTTCACACCGCCGCCCAAAGTGATGAGCGCCATCGTGCACGTGACGCCGGACGAGATGCCGGAGGGCGTGTCGGCCC
GCACGCTCGAACGCCTGACCGAAGCTGCCTTCGGCCAACGCCGCAAGATGCTGCGCCAGAGCCTGAAAGGCGTACCCGGC
GCGGTGGAGACATTGGCTGAGGTTGCCATCGAAGAGACGCGGCGGGCCGAGACGGTGACGGTGGAAGAGTTTGTGGCGCT
GGCGCGACGCCTCGGCGCTTCGCGACCCTCATCCAACTCCGGCTAA

Upstream 100 bases:

>100_bases
CATGGAACAGCCTTCGACATTGCCGGACGCGGCACCGCGCATCCGGGCGCCATGGTTTTCGCTTTGCTCATGGCAGGCGA
ATGCGCAGCGCGCCGGGCCG

Downstream 100 bases:

>100_bases
GCGCTCAAGCGGCAAGCCTGCGTATCCTTCTCCCACCGGGAGAAGCACAGCGCAGCTTGCGCGCGCATCGCGCTGGCGAA
GCTTGGATGAGGGTGACCGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MPELPPLRDVIAKHGLSASKALGQNFLFDAQLLDRIAGIPGGLENRAVLEIGPGPGGLTRALLKAGARVTAIEMDRRCLP
ALAELSEVYPGKLSVIHGDAMKLDHAELMGEPFAVVANLPYNVGTALFVRWLGGETWPPQWTSLTLMFQQEVAQRIVSTP
GTSAYGRLAVLAQWRSAASMPMKVHRSAFTPPPKVMSAIVHVTPDEMPEGVSARTLERLTEAAFGQRRKMLRQSLKGVPG
AVETLAEVAIEETRRAETVTVEEFVALARRLGASRPSSNSG

Sequences:

>Translated_281_residues
MPELPPLRDVIAKHGLSASKALGQNFLFDAQLLDRIAGIPGGLENRAVLEIGPGPGGLTRALLKAGARVTAIEMDRRCLP
ALAELSEVYPGKLSVIHGDAMKLDHAELMGEPFAVVANLPYNVGTALFVRWLGGETWPPQWTSLTLMFQQEVAQRIVSTP
GTSAYGRLAVLAQWRSAASMPMKVHRSAFTPPPKVMSAIVHVTPDEMPEGVSARTLERLTEAAFGQRRKMLRQSLKGVPG
AVETLAEVAIEETRRAETVTVEEFVALARRLGASRPSSNSG
>Mature_280_residues
PELPPLRDVIAKHGLSASKALGQNFLFDAQLLDRIAGIPGGLENRAVLEIGPGPGGLTRALLKAGARVTAIEMDRRCLPA
LAELSEVYPGKLSVIHGDAMKLDHAELMGEPFAVVANLPYNVGTALFVRWLGGETWPPQWTSLTLMFQQEVAQRIVSTPG
TSAYGRLAVLAQWRSAASMPMKVHRSAFTPPPKVMSAIVHVTPDEMPEGVSARTLERLTEAAFGQRRKMLRQSLKGVPGA
VETLAEVAIEETRRAETVTVEEFVALARRLGASRPSSNSG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=293, Percent_Identity=34.1296928327645, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI7657198, Length=261, Percent_Identity=29.8850574712644, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786236, Length=275, Percent_Identity=37.4545454545455, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=31.2080536912752, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25146882, Length=276, Percent_Identity=32.6086956521739, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=204, Percent_Identity=34.3137254901961, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=30.1038062283737, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21358017, Length=241, Percent_Identity=33.195020746888, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ERYLH (Q2N8W9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458669.1
- ProteinModelPortal:   Q2N8W9
- SMR:   Q2N8W9
- STRING:   Q2N8W9
- GeneID:   3869723
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_08900
- NMPDR:   fig|314225.3.peg.1263
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q2N8W9
- ProtClustDB:   PRK00274
- BioCyc:   ELIT314225:ELI_08900-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30275; Mature: 30144

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 25-25 BINDING 27-27 BINDING 52-52 BINDING 73-73 BINDING 99-99 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPPLRDVIAKHGLSASKALGQNFLFDAQLLDRIAGIPGGLENRAVLEIGPGPGGLTR
CCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
ALLKAGARVTAIEMDRRCLPALAELSEVYPGKLSVIHGDAMKLDHAELMGEPFAVVANLP
HHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCHHHHHCCCCEEEEECCC
YNVGTALFVRWLGGETWPPQWTSLTLMFQQEVAQRIVSTPGTSAYGRLAVLAQWRSAASM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PMKVHRSAFTPPPKVMSAIVHVTPDEMPEGVSARTLERLTEAAFGQRRKMLRQSLKGVPG
CHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVETLAEVAIEETRRAETVTVEEFVALARRLGASRPSSNSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PELPPLRDVIAKHGLSASKALGQNFLFDAQLLDRIAGIPGGLENRAVLEIGPGPGGLTR
CCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHH
ALLKAGARVTAIEMDRRCLPALAELSEVYPGKLSVIHGDAMKLDHAELMGEPFAVVANLP
HHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCHHHHHCCCCEEEEECCC
YNVGTALFVRWLGGETWPPQWTSLTLMFQQEVAQRIVSTPGTSAYGRLAVLAQWRSAASM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PMKVHRSAFTPPPKVMSAIVHVTPDEMPEGVSARTLERLTEAAFGQRRKMLRQSLKGVPG
CHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVETLAEVAIEETRRAETVTVEEFVALARRLGASRPSSNSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA