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The map label for this gene is argG

Identifier: 85374397

GI number: 85374397

Start: 1624331

End: 1625545

Strand: Direct

Name: argG

Synonym: ELI_07850

Alternate gene names: 85374397

Gene position: 1624331-1625545 (Clockwise)

Preceding gene: 85374396

Following gene: 85374398

Centisome position: 53.21

GC content: 59.75

Gene sequence:

>1215_bases
ATGCCAGATATCAAGCGTGTCGTCCTCGCCTATTCCGGCGGCCTCGATACTTCCGTCATTGCCAAATGGCTCGAAGTCGA
GCGCGGGCTGGAAGTGGTCACCTTCACTGCCGATCTTGGACAGGGAGAAGAACTCGAGCCTGCCCGCGCGAAGGCTCAAG
CGATGGGCATCCCGGATAAGCACATTTTCATTGAAGACCTGCGAGAAGAATTCGTTCGCGATTACGTCTTCCCGATGATG
CGCGCCAATGCGCGGTATGAAGGCGACTATCTGCTCGGTACTTCGATCGCCCGCCCGCTTATCTCCAAGCGGCTGGTCGA
GATTGCGCAAGAGACCGGGGCCGATGCCATCGCCCATGGCGCAACCGGTAAAGGTAATGACCAGGTCCGGTTCGAGCTCA
GCGCCTATGCGCTCGACCCCGACATCAAAGTCATCGCTCCGTGGCGCGAATGGGACCTGACCAGTCGCACTGCGCTGATC
GCCTGGGCCGAAAAGCACCAGATCCAGGTGCCGAAGGACAAGCGTGGCGAAAGCCCCTTTTCCACCGATGCCAATCTCCT
GCACACTTCCTCCGAAGGCAAAGTGCTGGAAGATCCATGGGAAGAGACACCCGACTACGTCTATTCGCGGACGGTCAATC
CCGAGGATGCGCCGGACGAGCCGGAATTTATCACCATCGACTTCGAGCGCGGCGATGGCATCGCCCTCAATGGCGAGGCG
ATGTCGCCCGCAAACCTGCTGACAGCGCTCAACGAGCTGGGCCGCAAGCATGGCATCGGGCGACTCGATCTGGTTGAGAA
CCGCTTTGTCGGCATGAAATCGCGCGGCATGTACGAAACGCCGGGCGGCGAGATCTATGCGCGCGCCCATCGCGGGATCG
AGCAGATCACGCTCGACCGCGGCGCGGCGCATCTCAAGGATGAACTGATGCCCAAATATGCCGAGCTCATCTACAACGGC
TTCTGGTTCAGCCCCGAACGCGAGATGCTGCAAGCCGCAATCGACCTGAGCCAGGACAAAGTCAGCGGGACCGTGCGGCT
CAAGCTCTACAAGGGCAATGCCGACGTCGTCGGTCGCAAGTCGCCCAATTCGCTCTATTCGGAAGCGCATGTGACATTCG
AGGATGACGCCGGTGCGTATGACCAGAGCGATGCAGAAGGATTTATCCGCTTGAATGCCTTGCGACTCAAGCTCCTCGGC
AAGCGCGATCGGTGA

Upstream 100 bases:

>100_bases
CCTGAACGTGCCGCCCGGTTGTGGCTTCCCAATCCAAACTTGATTGCCCCGCCCCGCCCCCGTGTGTAACCCGCGCGCCG
AACGCAGGAGCGCAAACGAA

Downstream 100 bases:

>100_bases
GTCGTTACGTTAATCACCAGCCTTGCTGCGGCGTAACACATGCGCTTTAGCGCTTCAGAAGCGCGCAATATTTGCACTGA
ATCGACGATCCGTTGAGTTA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPDIKRVVLAYSGGLDTSVIAKWLEVERGLEVVTFTADLGQGEELEPARAKAQAMGIPDKHIFIEDLREEFVRDYVFPMM
RANARYEGDYLLGTSIARPLISKRLVEIAQETGADAIAHGATGKGNDQVRFELSAYALDPDIKVIAPWREWDLTSRTALI
AWAEKHQIQVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDEPEFITIDFERGDGIALNGEA
MSPANLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIEQITLDRGAAHLKDELMPKYAELIYNG
FWFSPEREMLQAAIDLSQDKVSGTVRLKLYKGNADVVGRKSPNSLYSEAHVTFEDDAGAYDQSDAEGFIRLNALRLKLLG
KRDR

Sequences:

>Translated_404_residues
MPDIKRVVLAYSGGLDTSVIAKWLEVERGLEVVTFTADLGQGEELEPARAKAQAMGIPDKHIFIEDLREEFVRDYVFPMM
RANARYEGDYLLGTSIARPLISKRLVEIAQETGADAIAHGATGKGNDQVRFELSAYALDPDIKVIAPWREWDLTSRTALI
AWAEKHQIQVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDEPEFITIDFERGDGIALNGEA
MSPANLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIEQITLDRGAAHLKDELMPKYAELIYNG
FWFSPEREMLQAAIDLSQDKVSGTVRLKLYKGNADVVGRKSPNSLYSEAHVTFEDDAGAYDQSDAEGFIRLNALRLKLLG
KRDR
>Mature_403_residues
PDIKRVVLAYSGGLDTSVIAKWLEVERGLEVVTFTADLGQGEELEPARAKAQAMGIPDKHIFIEDLREEFVRDYVFPMMR
ANARYEGDYLLGTSIARPLISKRLVEIAQETGADAIAHGATGKGNDQVRFELSAYALDPDIKVIAPWREWDLTSRTALIA
WAEKHQIQVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDEPEFITIDFERGDGIALNGEAM
SPANLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIEQITLDRGAAHLKDELMPKYAELIYNGF
WFSPEREMLQAAIDLSQDKVSGTVRLKLYKGNADVVGRKSPNSLYSEAHVTFEDDAGAYDQSDAEGFIRLNALRLKLLGK
RDR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=400, Percent_Identity=47, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI16950633, Length=400, Percent_Identity=47, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1789563, Length=434, Percent_Identity=27.6497695852535, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=42.1307506053269, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21358151, Length=410, Percent_Identity=45.8536585365854, Blast_Score=341, Evalue=5e-94,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_ERYLH (Q2N9H9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458459.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q2N9H9
- SMR:   Q2N9H9
- STRING:   Q2N9H9
- GeneID:   3870341
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07850
- NMPDR:   fig|314225.3.peg.1471
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q2N9H9
- ProtClustDB:   PRK00509
- BioCyc:   ELIT314225:ELI_07850-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45157; Mature: 45026

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 90-90 BINDING 95-95 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIKRVVLAYSGGLDTSVIAKWLEVERGLEVVTFTADLGQGEELEPARAKAQAMGIPDK
CCCHHEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
HIFIEDLREEFVRDYVFPMMRANARYEGDYLLGTSIARPLISKRLVEIAQETGADAIAHG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELSAYALDPDIKVIAPWREWDLTSRTALIAWAEKHQIQVPKDKRGESPF
CCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCC
STDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDEPEFITIDFERGDGIALNGEA
CCCCCEEEECCCCCEECCCHHCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCC
MSPANLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIEQITLDR
CCHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCEECCCCCEEEHHHCCHHHEECCC
GAAHLKDELMPKYAELIYNGFWFSPEREMLQAAIDLSQDKVSGTVRLKLYKGNADVVGRK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEECCC
SPNSLYSEAHVTFEDDAGAYDQSDAEGFIRLNALRLKLLGKRDR
CCCHHHHCCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PDIKRVVLAYSGGLDTSVIAKWLEVERGLEVVTFTADLGQGEELEPARAKAQAMGIPDK
CCHHEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
HIFIEDLREEFVRDYVFPMMRANARYEGDYLLGTSIARPLISKRLVEIAQETGADAIAHG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELSAYALDPDIKVIAPWREWDLTSRTALIAWAEKHQIQVPKDKRGESPF
CCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCC
STDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDEPEFITIDFERGDGIALNGEA
CCCCCEEEECCCCCEECCCHHCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCC
MSPANLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIEQITLDR
CCHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCEECCCCCEEEHHHCCHHHEECCC
GAAHLKDELMPKYAELIYNGFWFSPEREMLQAAIDLSQDKVSGTVRLKLYKGNADVVGRK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEECCC
SPNSLYSEAHVTFEDDAGAYDQSDAEGFIRLNALRLKLLGKRDR
CCCHHHHCCEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA