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The map label for this gene is cfa [H]

Identifier: 85374396

GI number: 85374396

Start: 1622976

End: 1624235

Strand: Direct

Name: cfa [H]

Synonym: ELI_07845

Alternate gene names: 85374396

Gene position: 1622976-1624235 (Clockwise)

Preceding gene: 85374393

Following gene: 85374397

Centisome position: 53.17

GC content: 61.35

Gene sequence:

>1260_bases
ATGAGCGAGCGACTTCTCAAAAAGGTGCTGGGAAGCAAGATCACCCGCGGCACGCTCGAGGTGATCGGTCACAAGGGTGA
CAGCGAGACCTTCGGCACGCCAACGGAAGGATACCCCGATGTCACCGTGCGCTTCGCCGACCGCAAGACGGCACGCGACA
TTCTGCTCGATCCGCGACTTGGCGCAGGCGAATGCTTCATGGATGGTCGGCTGATAGTCGAGAAAGGCGGTGGGATCATG
GAGATGATCCAGCTGATCCGTGCCAACACGCCATGGGAGTCAGGACAACGGCTGCAGGCGCCATCAGTCTTGAAACGGGT
CAGGAATCGCCTGTCGTTCCTCGCCAGGTCTTTCAATCGACCCGCCAGCGCCAAACGCAACATCGCCCACCACTACGACA
TCGGCAATGATCTGTACGCGCTGTTCCTTGATAAAGAGCACTGGCAATACAGCTGCGCCTATTGGCCCGGCTATCCCGAA
GACCGCTCGATGTCGCTCGACGAAGCGCAGGAGCGCAAGCTGGCGCACATTGCCGCCAAGCTGGACCTGAAGCCCGGTAT
GCGCGTGGTCGAGATCGGCTGCGGCTGGGGTGGCCTGGCGATCTATCTCGCGCGCAAGTTCGACGTCCATGTCACCGGGA
TCACCCTGTCCGAAGAGCAGGCGAAGCTTGCCGTCGAAAGGGCGGAAGCTGCCGGCGTTGCGGACAAGGTCGACATCAAG
CTGATCGACTACCGCGCCTTCGCGGAAAGCGGAGGGAAGTTCGACCGCGTTGTCTCCATCGCCATGTTCGAGGCTGTCGG
GCGCCCGCAGTTCGAGACCTATTTCCGCTGCTGCGGAAACCTGATGAAGCAGAATGGGGTATTCCTCGTCCACACTATCG
GCCGCATGGGCGGGCCGGGGTCGACGGACGCTTTTACCAGCAAGTACATCTTCCCCGGCGGCTATATACCGGCGCTGAGC
GAAACCCTCGCGGCGAGCGAGAAGTTCCGCCTGATTGCGAGCGATATCGAGACGCTGCGCTTGCACTACGCACCCACCCT
GCGCGCTTGGTACGACCGCTGCATGGCCGACAAGGACGCCATCGTCGCCATGTATGACGAGCGCTTCTTCCGCATGTGGA
CCTTTTACCTCGCCGGTGCGACCGCGGCCTTCGAAAGTGGCGGCATGTGCAACTACCAGATCCAATACGTCCGCGACCGC
CATGCATTGCCGATCACGCGCGACTACATGGCGGAAGGGGAAAGGCAACTGCTGGCCTGA

Upstream 100 bases:

>100_bases
CCGGGTCGACATCGGTGATCTGCGTCTTTGCTTCCGCCATGAATGCGGCCATACCCCAAGTGCGTCACAAGCGAAATCAT
GCGATGAAGGGAGGCACTGC

Downstream 100 bases:

>100_bases
ACGTGCCGCCCGGTTGTGGCTTCCCAATCCAAACTTGATTGCCCCGCCCCGCCCCCGTGTGTAACCCGCGCGCCGAACGC
AGGAGCGCAAACGAAATGCC

Product: cyclopropane fatty acid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSERLLKKVLGSKITRGTLEVIGHKGDSETFGTPTEGYPDVTVRFADRKTARDILLDPRLGAGECFMDGRLIVEKGGGIM
EMIQLIRANTPWESGQRLQAPSVLKRVRNRLSFLARSFNRPASAKRNIAHHYDIGNDLYALFLDKEHWQYSCAYWPGYPE
DRSMSLDEAQERKLAHIAAKLDLKPGMRVVEIGCGWGGLAIYLARKFDVHVTGITLSEEQAKLAVERAEAAGVADKVDIK
LIDYRAFAESGGKFDRVVSIAMFEAVGRPQFETYFRCCGNLMKQNGVFLVHTIGRMGGPGSTDAFTSKYIFPGGYIPALS
ETLAASEKFRLIASDIETLRLHYAPTLRAWYDRCMADKDAIVAMYDERFFRMWTFYLAGATAAFESGGMCNYQIQYVRDR
HALPITRDYMAEGERQLLA

Sequences:

>Translated_419_residues
MSERLLKKVLGSKITRGTLEVIGHKGDSETFGTPTEGYPDVTVRFADRKTARDILLDPRLGAGECFMDGRLIVEKGGGIM
EMIQLIRANTPWESGQRLQAPSVLKRVRNRLSFLARSFNRPASAKRNIAHHYDIGNDLYALFLDKEHWQYSCAYWPGYPE
DRSMSLDEAQERKLAHIAAKLDLKPGMRVVEIGCGWGGLAIYLARKFDVHVTGITLSEEQAKLAVERAEAAGVADKVDIK
LIDYRAFAESGGKFDRVVSIAMFEAVGRPQFETYFRCCGNLMKQNGVFLVHTIGRMGGPGSTDAFTSKYIFPGGYIPALS
ETLAASEKFRLIASDIETLRLHYAPTLRAWYDRCMADKDAIVAMYDERFFRMWTFYLAGATAAFESGGMCNYQIQYVRDR
HALPITRDYMAEGERQLLA
>Mature_418_residues
SERLLKKVLGSKITRGTLEVIGHKGDSETFGTPTEGYPDVTVRFADRKTARDILLDPRLGAGECFMDGRLIVEKGGGIME
MIQLIRANTPWESGQRLQAPSVLKRVRNRLSFLARSFNRPASAKRNIAHHYDIGNDLYALFLDKEHWQYSCAYWPGYPED
RSMSLDEAQERKLAHIAAKLDLKPGMRVVEIGCGWGGLAIYLARKFDVHVTGITLSEEQAKLAVERAEAAGVADKVDIKL
IDYRAFAESGGKFDRVVSIAMFEAVGRPQFETYFRCCGNLMKQNGVFLVHTIGRMGGPGSTDAFTSKYIFPGGYIPALSE
TLAASEKFRLIASDIETLRLHYAPTLRAWYDRCMADKDAIVAMYDERFFRMWTFYLAGATAAFESGGMCNYQIQYVRDRH
ALPITRDYMAEGERQLLA

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=369, Percent_Identity=37.3983739837398, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI133904226, Length=373, Percent_Identity=30.0268096514745, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47097; Mature: 46965

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERLLKKVLGSKITRGTLEVIGHKGDSETFGTPTEGYPDVTVRFADRKTARDILLDPRL
CCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHEECCCC
GAGECFMDGRLIVEKGGGIMEMIQLIRANTPWESGQRLQAPSVLKRVRNRLSFLARSFNR
CCCCEEECCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PASAKRNIAHHYDIGNDLYALFLDKEHWQYSCAYWPGYPEDRSMSLDEAQERKLAHIAAK
CCHHHHCCHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
LDLKPGMRVVEIGCGWGGLAIYLARKFDVHVTGITLSEEQAKLAVERAEAAGVADKVDIK
CCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCEEE
LIDYRAFAESGGKFDRVVSIAMFEAVGRPQFETYFRCCGNLMKQNGVFLVHTIGRMGGPG
EEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
STDAFTSKYIFPGGYIPALSETLAASEKFRLIASDIETLRLHYAPTLRAWYDRCMADKDA
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCC
IVAMYDERFFRMWTFYLAGATAAFESGGMCNYQIQYVRDRHALPITRDYMAEGERQLLA
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHCCHHHHCC
>Mature Secondary Structure 
SERLLKKVLGSKITRGTLEVIGHKGDSETFGTPTEGYPDVTVRFADRKTARDILLDPRL
CHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHEECCCC
GAGECFMDGRLIVEKGGGIMEMIQLIRANTPWESGQRLQAPSVLKRVRNRLSFLARSFNR
CCCCEEECCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PASAKRNIAHHYDIGNDLYALFLDKEHWQYSCAYWPGYPEDRSMSLDEAQERKLAHIAAK
CCHHHHCCHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
LDLKPGMRVVEIGCGWGGLAIYLARKFDVHVTGITLSEEQAKLAVERAEAAGVADKVDIK
CCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCEEE
LIDYRAFAESGGKFDRVVSIAMFEAVGRPQFETYFRCCGNLMKQNGVFLVHTIGRMGGPG
EEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
STDAFTSKYIFPGGYIPALSETLAASEKFRLIASDIETLRLHYAPTLRAWYDRCMADKDA
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCC
IVAMYDERFFRMWTFYLAGATAAFESGGMCNYQIQYVRDRHALPITRDYMAEGERQLLA
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]