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The map label for this gene is purF [H]

Identifier: 85374230

GI number: 85374230

Start: 1454502

End: 1455974

Strand: Direct

Name: purF [H]

Synonym: ELI_07015

Alternate gene names: 85374230

Gene position: 1454502-1455974 (Clockwise)

Preceding gene: 85374219

Following gene: 85374231

Centisome position: 47.65

GC content: 63.48

Gene sequence:

>1473_bases
ATGATCACCAGCCATCCCTTTTACGACGAGCACGGCGACAAGCTGCGCGAAGAATGCGGAGTCTTCGGCGCAATCAATGC
CACCGATGCATCCGCCGCGACCGCGCTCGGGCTGCATGCCCTGCAGCATCGCGGGCAGGAAGCCGTCGGCATCACCAGCT
TCGATGGCGAACACTTCTACCAGCGTCGCGGGCTCGGCCACGTCGCGGAAAACTTTTCCTCGCCTGAGGCGATAGCCGAG
TTGCCCGGCACCATGGCCGGCGGGCACGTGCGCTATTCGACCACCGGCGGGGCCGGCCTGCGCAATGTCCAGCCACTCTA
TGCCGACCTCGCCTTCGGCGGTTTCTCGATAGCGCATAACGGCAATATCTCGAACGCCGAGACGCTGCGCGCGGAACTTG
TCGCCAAGGGCGCGATCTTCCAGTCGACGAGCGACACCGAAGTCATCATCCACCTGGTGGCAACGAGCCGCTATCCGACC
CTGCGCGATCGGCTGGTCGACGCCCTGCGCCTCGTCGAAGGCGCCTATGCGCTGATCGTGATGACGCCCGAAGGCATGAT
CGCCTGCCGCGATCCTCTCGGCATTCGCCCGCTGCAGATGGGTCGGATGGGCAATGCCGTGGCTTTCGCCAGCGAAACTG
TCGCCTTCGATGTCGTCGGCGCGAAATTCGATCGGCAGGTCGAGCCGGGCGAACTGATCGAAGTCGATTTCGACGGCAAT
GTCACGAGCTTCCGACCGTTCGGCGAAGGCGCGGCGCGGCCCTGTATCTTCGAGCATGTCTATTTCAGCCGCCCGGACTC
GATCTTCGACGGGCGCAGCGTTTACGAAGCGCGCAAGGCCATCGGCCAGCAACTCGCGATCGAAAGCCCCTGCGACGCCG
ATCTGGTCGTTCCCGTTCCCGACAGCGGCGTGCCTGCTGCCATCGGCTATGCGCAGCAATCGGGCGTGCCTTTCGAACTC
GGCATCATCCGCAGTCACTATGTCGGACGCACCTTCATCCAGCCTTCCGATGGCGCGCGGCATTCGGGCGTGAAGCGCAA
GCACAATGCCAATCGCGCCCTCGTCGAAGGCAAGCGGATCGTGCTGATCGACGATTCCATCGTTCGCGGCACCACCTCGA
TGAAGATCGTCGAGATGATGCGTGAAGCCGGGGCGACCGAGGTCCACTTCCGCGTCGCCAGCCCGCCTACCGCGCATAGC
TGCTTCTACGGGGTCGATACGCCAGAGCGGAGCAAGCTGCTCGCCGCTCGAATGGACGTCGAGCCGATGCGCGAATTCAT
TCGCGCCGACAGCCTCGCCTTTGTTTCCATCGACGGCCTGTACCGCGCCGTGGGCGAGAAGCCGCGCAACAGCAATTGTC
CGCAATTCTGCGATGCCTGCTTCACCGGCGATTACCCCACCTCGCTGACCGACCTGAGCATGAAGCAGGACGGCGCGGCG
CAGCTTTCTTTTCCTGTCAACAAAGTCGCTTAA

Upstream 100 bases:

>100_bases
GGTTATAACCGGGTAAGGATTGCGGTTCTTCGCCCCTTGTAATCGTGTCGTAACATCGCCATTGGGTTCGCGCACGGGGG
CAGCAGCAGGCGCGACGGTC

Downstream 100 bases:

>100_bases
ATGACTGAACAGAAATCTCTTTCGGGCAAGATTGCCCTCGTCACCGGCGCGTCCAAGGGAATTGGCGCAGCCACGGCCAA
GGCGCTCGGTGCAGCAGGCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MITSHPFYDEHGDKLREECGVFGAINATDASAATALGLHALQHRGQEAVGITSFDGEHFYQRRGLGHVAENFSSPEAIAE
LPGTMAGGHVRYSTTGGAGLRNVQPLYADLAFGGFSIAHNGNISNAETLRAELVAKGAIFQSTSDTEVIIHLVATSRYPT
LRDRLVDALRLVEGAYALIVMTPEGMIACRDPLGIRPLQMGRMGNAVAFASETVAFDVVGAKFDRQVEPGELIEVDFDGN
VTSFRPFGEGAARPCIFEHVYFSRPDSIFDGRSVYEARKAIGQQLAIESPCDADLVVPVPDSGVPAAIGYAQQSGVPFEL
GIIRSHYVGRTFIQPSDGARHSGVKRKHNANRALVEGKRIVLIDDSIVRGTTSMKIVEMMREAGATEVHFRVASPPTAHS
CFYGVDTPERSKLLAARMDVEPMREFIRADSLAFVSIDGLYRAVGEKPRNSNCPQFCDACFTGDYPTSLTDLSMKQDGAA
QLSFPVNKVA

Sequences:

>Translated_490_residues
MITSHPFYDEHGDKLREECGVFGAINATDASAATALGLHALQHRGQEAVGITSFDGEHFYQRRGLGHVAENFSSPEAIAE
LPGTMAGGHVRYSTTGGAGLRNVQPLYADLAFGGFSIAHNGNISNAETLRAELVAKGAIFQSTSDTEVIIHLVATSRYPT
LRDRLVDALRLVEGAYALIVMTPEGMIACRDPLGIRPLQMGRMGNAVAFASETVAFDVVGAKFDRQVEPGELIEVDFDGN
VTSFRPFGEGAARPCIFEHVYFSRPDSIFDGRSVYEARKAIGQQLAIESPCDADLVVPVPDSGVPAAIGYAQQSGVPFEL
GIIRSHYVGRTFIQPSDGARHSGVKRKHNANRALVEGKRIVLIDDSIVRGTTSMKIVEMMREAGATEVHFRVASPPTAHS
CFYGVDTPERSKLLAARMDVEPMREFIRADSLAFVSIDGLYRAVGEKPRNSNCPQFCDACFTGDYPTSLTDLSMKQDGAA
QLSFPVNKVA
>Mature_490_residues
MITSHPFYDEHGDKLREECGVFGAINATDASAATALGLHALQHRGQEAVGITSFDGEHFYQRRGLGHVAENFSSPEAIAE
LPGTMAGGHVRYSTTGGAGLRNVQPLYADLAFGGFSIAHNGNISNAETLRAELVAKGAIFQSTSDTEVIIHLVATSRYPT
LRDRLVDALRLVEGAYALIVMTPEGMIACRDPLGIRPLQMGRMGNAVAFASETVAFDVVGAKFDRQVEPGELIEVDFDGN
VTSFRPFGEGAARPCIFEHVYFSRPDSIFDGRSVYEARKAIGQQLAIESPCDADLVVPVPDSGVPAAIGYAQQSGVPFEL
GIIRSHYVGRTFIQPSDGARHSGVKRKHNANRALVEGKRIVLIDDSIVRGTTSMKIVEMMREAGATEVHFRVASPPTAHS
CFYGVDTPERSKLLAARMDVEPMREFIRADSLAFVSIDGLYRAVGEKPRNSNCPQFCDACFTGDYPTSLTDLSMKQDGAA
QLSFPVNKVA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=37.328094302554, Blast_Score=332, Evalue=4e-91,
Organism=Escherichia coli, GI1788651, Length=474, Percent_Identity=37.3417721518987, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1790167, Length=277, Percent_Identity=29.9638989169675, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=37.5782881002088, Blast_Score=304, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=485, Percent_Identity=33.8144329896907, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=41.3087934560327, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=39.6793587174349, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=41.6309012875536, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52945; Mature: 52945

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSHPFYDEHGDKLREECGVFGAINATDASAATALGLHALQHRGQEAVGITSFDGEHFY
CCCCCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
QRRGLGHVAENFSSPEAIAELPGTMAGGHVRYSTTGGAGLRNVQPLYADLAFGGFSIAHN
HHCCCHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEC
GNISNAETLRAELVAKGAIFQSTSDTEVIIHLVATSRYPTLRDRLVDALRLVEGAYALIV
CCCCCHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
MTPEGMIACRDPLGIRPLQMGRMGNAVAFASETVAFDVVGAKFDRQVEPGELIEVDFDGN
ECCCCCEEECCCCCCCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEECCCC
VTSFRPFGEGAARPCIFEHVYFSRPDSIFDGRSVYEARKAIGQQLAIESPCDADLVVPVP
CCEECCCCCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DSGVPAAIGYAQQSGVPFELGIIRSHYVGRTFIQPSDGARHSGVKRKHNANRALVEGKRI
CCCCCHHCCHHHHCCCCEEEEEHHHHCCCCEEECCCCCCCCCCCCHHCCCCCCEECCCEE
VLIDDSIVRGTTSMKIVEMMREAGATEVHFRVASPPTAHSCFYGVDTPERSKLLAARMDV
EEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHCCH
EPMREFIRADSLAFVSIDGLYRAVGEKPRNSNCPQFCDACFTGDYPTSLTDLSMKQDGAA
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
QLSFPVNKVA
EEECCHHHCC
>Mature Secondary Structure
MITSHPFYDEHGDKLREECGVFGAINATDASAATALGLHALQHRGQEAVGITSFDGEHFY
CCCCCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
QRRGLGHVAENFSSPEAIAELPGTMAGGHVRYSTTGGAGLRNVQPLYADLAFGGFSIAHN
HHCCCHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEC
GNISNAETLRAELVAKGAIFQSTSDTEVIIHLVATSRYPTLRDRLVDALRLVEGAYALIV
CCCCCHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
MTPEGMIACRDPLGIRPLQMGRMGNAVAFASETVAFDVVGAKFDRQVEPGELIEVDFDGN
ECCCCCEEECCCCCCCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEECCCC
VTSFRPFGEGAARPCIFEHVYFSRPDSIFDGRSVYEARKAIGQQLAIESPCDADLVVPVP
CCEECCCCCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DSGVPAAIGYAQQSGVPFELGIIRSHYVGRTFIQPSDGARHSGVKRKHNANRALVEGKRI
CCCCCHHCCHHHHCCCCEEEEEHHHHCCCCEEECCCCCCCCCCCCHHCCCCCCEECCCEE
VLIDDSIVRGTTSMKIVEMMREAGATEVHFRVASPPTAHSCFYGVDTPERSKLLAARMDV
EEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHCCH
EPMREFIRADSLAFVSIDGLYRAVGEKPRNSNCPQFCDACFTGDYPTSLTDLSMKQDGAA
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
QLSFPVNKVA
EEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA