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The map label for this gene is mltB [H]

Identifier: 85374067

GI number: 85374067

Start: 1289421

End: 1290452

Strand: Direct

Name: mltB [H]

Synonym: ELI_06200

Alternate gene names: 85374067

Gene position: 1289421-1290452 (Clockwise)

Preceding gene: 85374066

Following gene: 85374068

Centisome position: 42.24

GC content: 61.53

Gene sequence:

>1032_bases
ATGCTGCAAATCATGTCCCTCAAGCGCCTCCTTCTCGCCCTCTCGGCCATGGCACTCGCCACGCCGCTCGCCCCCGTGTC
CGCACAGAATCTCTCTTTCGACGCCTACATGCAATTGGTGATGGCGAAGGCGAGGGCGCAAGGCGTCAGCGAGGCAACTA
TTCAGCGCATGACGTCAGGGCTCACACCCAACCAACGGGTCATCGACCTCGATCGCGGCCAACCCGGTAGCCCGACGCGC
CGCGGCTATCCCGCGATGCGCCCCTATCTCGATACCCATGTGAACCCGATCCGTATCAGCCGCGGGCGCGACATGTATGC
CGCAACGCGCGGTCTCCATGGCCGGATCGAGCGCGAATACGGGGTGCCGATCGAGATGCTGATGGCGATCTGGGGGCACG
AGACCAATTATGGCGCGATCAAGGGAAACTTCGATCTGGCCCGCTCTCTCGCCACACTCGCGTGGGAAGGCCGCCGTCGC
GACCTGTTCGAAGGCGAACTGATCGCGCTGATGAAGATTGCGGACCGGGGTTTTCCTCGTTCGCAACTCGTCGGCAGCTA
TGCCGGCGCCTTCGGCAATCCGCAATTCCTGCCGAGCGTCTACTTGCGCCTTGCCACCGATGGCGACGGGGACGGGCGGG
CCGACATCTTCAACAATCGCGCCGATACGCTTGCTTCGATCGCGCGCTATTTCCAAGATTCGGGCTGGCGACAGGGCCAG
CCATGGGGCGTGCGCGCCTCTCTTCCCGGCGGCTTCGACGTCGATGCCTACAGGACCAAGATCAATGCTCCGGTCTGCTC
GCGCGTCCACGAGAGACATAGCGAGTACAAGACCGTGCGCGAATGGCGCGAACTCGGCGTTCAGCCGCAGAAATATTTGG
CGGATGACGTCCTGGTGACGCTGTTCCAGCCCGACGGTCCGGGCACACCGGCTTGGCTCTTAACCTCAAATTATCGAGTT
ATCCTCGAATATAACTGCTCCAATTACTACGCGATGAGTGTGGGGCTGTTAGCCGATGAGATTGCTCAATGA

Upstream 100 bases:

>100_bases
TCGCGATAAGCGCCTGACTCTCCATCGTTTCAGGCCTCACTGCAGAACGTTTCGTCGAAAAGCTTCGCTTCGGTCATTGG
CCTTTCATCTTGGCGGTGAC

Downstream 100 bases:

>100_bases
TGTTCGCAAGCTTGGCCTTTTTTTCGGTCTAGGCGTTCTGCTGTCCGCTTGCGGTGGCGGTGCTTCGCTTCCGGAGTTGG
CCAGCACGGAAACTTCTGCT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLQIMSLKRLLLALSAMALATPLAPVSAQNLSFDAYMQLVMAKARAQGVSEATIQRMTSGLTPNQRVIDLDRGQPGSPTR
RGYPAMRPYLDTHVNPIRISRGRDMYAATRGLHGRIEREYGVPIEMLMAIWGHETNYGAIKGNFDLARSLATLAWEGRRR
DLFEGELIALMKIADRGFPRSQLVGSYAGAFGNPQFLPSVYLRLATDGDGDGRADIFNNRADTLASIARYFQDSGWRQGQ
PWGVRASLPGGFDVDAYRTKINAPVCSRVHERHSEYKTVREWRELGVQPQKYLADDVLVTLFQPDGPGTPAWLLTSNYRV
ILEYNCSNYYAMSVGLLADEIAQ

Sequences:

>Translated_343_residues
MLQIMSLKRLLLALSAMALATPLAPVSAQNLSFDAYMQLVMAKARAQGVSEATIQRMTSGLTPNQRVIDLDRGQPGSPTR
RGYPAMRPYLDTHVNPIRISRGRDMYAATRGLHGRIEREYGVPIEMLMAIWGHETNYGAIKGNFDLARSLATLAWEGRRR
DLFEGELIALMKIADRGFPRSQLVGSYAGAFGNPQFLPSVYLRLATDGDGDGRADIFNNRADTLASIARYFQDSGWRQGQ
PWGVRASLPGGFDVDAYRTKINAPVCSRVHERHSEYKTVREWRELGVQPQKYLADDVLVTLFQPDGPGTPAWLLTSNYRV
ILEYNCSNYYAMSVGLLADEIAQ
>Mature_343_residues
MLQIMSLKRLLLALSAMALATPLAPVSAQNLSFDAYMQLVMAKARAQGVSEATIQRMTSGLTPNQRVIDLDRGQPGSPTR
RGYPAMRPYLDTHVNPIRISRGRDMYAATRGLHGRIEREYGVPIEMLMAIWGHETNYGAIKGNFDLARSLATLAWEGRRR
DLFEGELIALMKIADRGFPRSQLVGSYAGAFGNPQFLPSVYLRLATDGDGDGRADIFNNRADTLASIARYFQDSGWRQGQ
PWGVRASLPGGFDVDAYRTKINAPVCSRVHERHSEYKTVREWRELGVQPQKYLADDVLVTLFQPDGPGTPAWLLTSNYRV
ILEYNCSNYYAMSVGLLADEIAQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=319, Percent_Identity=28.5266457680251, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 38252; Mature: 38252

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQIMSLKRLLLALSAMALATPLAPVSAQNLSFDAYMQLVMAKARAQGVSEATIQRMTSG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHC
LTPNQRVIDLDRGQPGSPTRRGYPAMRPYLDTHVNPIRISRGRDMYAATRGLHGRIEREY
CCCCCEEEECCCCCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCC
GVPIEMLMAIWGHETNYGAIKGNFDLARSLATLAWEGRRRDLFEGELIALMKIADRGFPR
CCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
SQLVGSYAGAFGNPQFLPSVYLRLATDGDGDGRADIFNNRADTLASIARYFQDSGWRQGQ
HHHHHHHHCCCCCCCHHHEEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCC
PWGVRASLPGGFDVDAYRTKINAPVCSRVHERHSEYKTVREWRELGVQPQKYLADDVLVT
CCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEE
LFQPDGPGTPAWLLTSNYRVILEYNCSNYYAMSVGLLADEIAQ
EECCCCCCCCEEEEECCCEEEEEECCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MLQIMSLKRLLLALSAMALATPLAPVSAQNLSFDAYMQLVMAKARAQGVSEATIQRMTSG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHC
LTPNQRVIDLDRGQPGSPTRRGYPAMRPYLDTHVNPIRISRGRDMYAATRGLHGRIEREY
CCCCCEEEECCCCCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCC
GVPIEMLMAIWGHETNYGAIKGNFDLARSLATLAWEGRRRDLFEGELIALMKIADRGFPR
CCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
SQLVGSYAGAFGNPQFLPSVYLRLATDGDGDGRADIFNNRADTLASIARYFQDSGWRQGQ
HHHHHHHHCCCCCCCHHHEEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCC
PWGVRASLPGGFDVDAYRTKINAPVCSRVHERHSEYKTVREWRELGVQPQKYLADDVLVT
CCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEE
LFQPDGPGTPAWLLTSNYRVILEYNCSNYYAMSVGLLADEIAQ
EECCCCCCCCEEEEECCCEEEEEECCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]