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The map label for this gene is ilvE [H]

Identifier: 85374065

GI number: 85374065

Start: 1287746

End: 1288591

Strand: Direct

Name: ilvE [H]

Synonym: ELI_06190

Alternate gene names: 85374065

Gene position: 1287746-1288591 (Clockwise)

Preceding gene: 85374059

Following gene: 85374066

Centisome position: 42.19

GC content: 59.46

Gene sequence:

>846_bases
ATGGTGCCTCGCACGGAAGCGACCGTCTCCGTATTCGACAGCGGTTTCATGCTCGGTGATGGCGTTTGGGAGGGGCTGCG
CTTGCACAAAGGCAGGATTGCTTTCCTCGATGCCCACCTCGATCGGCTCTACGAAGGCGCGAAGGCGATCGCGATGGATA
TCGGCCTGTCGCGTGAGGAGCTCATTGCGAGGCTGGAAGAAACGATAGATTCCAATGCCATGCGGGGCGAAGCTGGAGTT
CATATCCGCCTGATGGTCACGCGCGGCATTCGCTCGACGCCGTATCAGGATCCACGCGTCGTGATCTCACCGGCCACAAT
CGTGATAATTCCAGAGTACAAAGCGCCGCTGCCCTCTACGATCGAAACCGGCATTCGCCTCTTCACCGTTCATGTCCGCC
GCGGGGACCCGGCCGTGCAGGACCAGAAGCTTAATTCGCACTCCAAGCTCAACTGCATCACCGCATGTATTCAGGCGACG
CAGGCGAGCGCTGACGAGGCGCTGATGCTCGATCCGCACGGCTTCGTCGCGACCTGCAATTCGACCCACTTCTTCATCGT
CCGCAAGGGCGAGGTGTGGACCTCGAGTGGCGATTATTGCTTGGGCGGGATTACGCGCTCGAATGTCATTCAGGTTTGCC
GCGAGGAAGGCATACCGGTGTTCGAGAAGAACTTTTCCCTGACCGACGTCTATGGCGCCGACGAAGCCTTCGTTACCGGA
ACCTTCGCTGGCGTCGTGCCGGTAACCGAGATCGACGGGCGCAAGATGACTGAGGGGAGGGGGCCGATGGTCGAACGATT
GCAGGACCTCTACAAGGCTCTCATGGATCGGGATGTGACGTCATGA

Upstream 100 bases:

>100_bases
CCCGCATTCAGCACCCTAGAACCTCCTCATGGCTCACGGCTCTCACGATTTCGCACCCGATCCCCGTAATGAGGACATCC
TCATAAGCGTCAACGGAACG

Downstream 100 bases:

>100_bases
CGTCAGCGCAACCCACTGAAACTATTCGGATTGCTATGTGGAGCGGGCCGCGCAACATTTCCACCGCGATGATGCGCAGT
TTCGGTGCCCGCAGCGACTG

Product: branched-chain amino acid aminotransferase, putative

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MVPRTEATVSVFDSGFMLGDGVWEGLRLHKGRIAFLDAHLDRLYEGAKAIAMDIGLSREELIARLEETIDSNAMRGEAGV
HIRLMVTRGIRSTPYQDPRVVISPATIVIIPEYKAPLPSTIETGIRLFTVHVRRGDPAVQDQKLNSHSKLNCITACIQAT
QASADEALMLDPHGFVATCNSTHFFIVRKGEVWTSSGDYCLGGITRSNVIQVCREEGIPVFEKNFSLTDVYGADEAFVTG
TFAGVVPVTEIDGRKMTEGRGPMVERLQDLYKALMDRDVTS

Sequences:

>Translated_281_residues
MVPRTEATVSVFDSGFMLGDGVWEGLRLHKGRIAFLDAHLDRLYEGAKAIAMDIGLSREELIARLEETIDSNAMRGEAGV
HIRLMVTRGIRSTPYQDPRVVISPATIVIIPEYKAPLPSTIETGIRLFTVHVRRGDPAVQDQKLNSHSKLNCITACIQAT
QASADEALMLDPHGFVATCNSTHFFIVRKGEVWTSSGDYCLGGITRSNVIQVCREEGIPVFEKNFSLTDVYGADEAFVTG
TFAGVVPVTEIDGRKMTEGRGPMVERLQDLYKALMDRDVTS
>Mature_281_residues
MVPRTEATVSVFDSGFMLGDGVWEGLRLHKGRIAFLDAHLDRLYEGAKAIAMDIGLSREELIARLEETIDSNAMRGEAGV
HIRLMVTRGIRSTPYQDPRVVISPATIVIIPEYKAPLPSTIETGIRLFTVHVRRGDPAVQDQKLNSHSKLNCITACIQAT
QASADEALMLDPHGFVATCNSTHFFIVRKGEVWTSSGDYCLGGITRSNVIQVCREEGIPVFEKNFSLTDVYGADEAFVTG
TFAGVVPVTEIDGRKMTEGRGPMVERLQDLYKALMDRDVTS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=282, Percent_Identity=27.3049645390071, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787338, Length=270, Percent_Identity=25.5555555555556, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 30918; Mature: 30918

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRTEATVSVFDSGFMLGDGVWEGLRLHKGRIAFLDAHLDRLYEGAKAIAMDIGLSREE
CCCCCCCEEEEECCCEEECCHHHCCCEECCCEEEEEHHHHHHHHHCHHEEEEECCCCHHH
LIARLEETIDSNAMRGEAGVHIRLMVTRGIRSTPYQDPRVVISPATIVIIPEYKAPLPST
HHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCEEEEEEECCCCCCCHH
IETGIRLFTVHVRRGDPAVQDQKLNSHSKLNCITACIQATQASADEALMLDPHGFVATCN
HHCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEC
STHFFIVRKGEVWTSSGDYCLGGITRSNVIQVCREEGIPVFEKNFSLTDVYGADEAFVTG
CEEEEEEECCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCEEEEE
TFAGVVPVTEIDGRKMTEGRGPMVERLQDLYKALMDRDVTS
EEECEEEEEECCCCEECCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVPRTEATVSVFDSGFMLGDGVWEGLRLHKGRIAFLDAHLDRLYEGAKAIAMDIGLSREE
CCCCCCCEEEEECCCEEECCHHHCCCEECCCEEEEEHHHHHHHHHCHHEEEEECCCCHHH
LIARLEETIDSNAMRGEAGVHIRLMVTRGIRSTPYQDPRVVISPATIVIIPEYKAPLPST
HHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCEEEEEEECCCCCCCHH
IETGIRLFTVHVRRGDPAVQDQKLNSHSKLNCITACIQATQASADEALMLDPHGFVATCN
HHCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEC
STHFFIVRKGEVWTSSGDYCLGGITRSNVIQVCREEGIPVFEKNFSLTDVYGADEAFVTG
CEEEEEEECCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCEEEEE
TFAGVVPVTEIDGRKMTEGRGPMVERLQDLYKALMDRDVTS
EEECEEEEEECCCCEECCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]