Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 85374045

GI number: 85374045

Start: 1260226

End: 1261866

Strand: Direct

Name: shiA [H]

Synonym: ELI_06090

Alternate gene names: 85374045

Gene position: 1260226-1261866 (Clockwise)

Preceding gene: 85374044

Following gene: 85374046

Centisome position: 41.29

GC content: 60.21

Gene sequence:

>1641_bases
ATGAGAGACGCAGCAGCATCGAAGGGACGCGAGCCGAGCGAGAACGAGATCAAACTCGTCATCGGTGCCTCGTCCGCCGG
GACCATCTTCGAATGGTACGATTTCTTCATCTACGGGACGCTCGCCTATATCCTCAAGGATGCGTTCTACGACGTCGACA
ACGAAACGCTGGGCTTGTTGCTGGTCTGGTCGACTTTTGCCGTGGGATTCGCCTTCCGCCCGATCGGCGCCGTGCTGTTC
GGTTTCCTTGGAGACAAGTTGGGGCGCAAATATACCTTCCTCGTCACAGTGACCCTGATGGGTATAGCAACCGCAGGGGT
CGGCCTGATACCGACCGTCGCGACGATCGGAATCGCGGCTCCGATCATCGTTATCCTGTTGCGCGTGATTCAGGGCCTGG
CGCTGGGCGGGGAATACGGTGGCGCTGCCATCTATGTTGCCGAGCATGCCCCGCCGGAAAAGCGCGGCTTCTACACCAGC
TTCATCCAGGCCAGTGTGGCCGGCGGCTTCGTCCTTTCTATTGCTGTCGTATTGGCGTGCCGCTTTCTTATTCCCGAGGA
TGATTTCGTGGCATGGGGCTGGCGCGTGCCGTTCCTGCTGTCGATCATTCTGCTTGGCATTTCGCTATGGATGCGGCTCA
AACTGTCGGAAAGTCCGGTCTTTCAGGCGATGAAGGCAGAAGGCCAGATCGCCGGCAATCCCTTCGTCGAAAGTTTCACA
TATCCCGGCAACAAGAAGCGCATCTTCGTCGCCCTGTTCGGCGTGACCGGCATCCTGACCACGATCTGGTACACGGCCTT
CTTCAGCGGGATGAGCTTCTTGCGCGGGCCGATGCATGTGGCCGACCTGACCGTGGAATTGATCCTGTTCGTGTCCGGCC
TGATCGCCATGACTTTCTACGTCATGATCGGCAAATGGTCCGACACCATAGGACGCAAGAAACCAATCATGATCGGTGCA
GTGTTGACGCTCCTGCTGCTGTTTCCGGCCTTCTGGGGCCTCGGTAGTCTTGCCAATCCCGGGCTTGAGCGAGCTGCGGC
GGAAACGCCGATCACGGTCAGCGGGCCGCAATGCACGACCGATCCCTTCGCCGACCTCTTCGACCGCGAACAAACCGATT
GCGGCAAGGTTCTCGAAACTCTGACCGCGTCGGGCGTGCCATATACTCTGGAAGCCGGCGAAACGCTGACGCTGAGTGCA
GGCGGTCAGGCCATCGCGATCGATCCTGCATGGTTCGAAGACGGCACCGCTCGGCGCGAGGGAATCCGCGCGGCGCTGGG
CGAATACGGATTCGACTTTTCGAAGCAGCAGCCCGGCATGGGCAATATCCTCGGCATCGTGGCAATTCTGCTCGGTCTGG
GCATGCTTTCCGCGCTGACCTACGGCTCGGTCGCGGCACTGCTGTCGGAGATGTTCCCGCCGCGCATCCGCTACAGTTCC
ATGTCGATACCCTATCACATCGGGGCCGGCTATCTCGGCGGTTTCCTGCCGCTCATCGCGGGCGTGATCGTTGCCAGTAC
AGGTAATATCTATTCGGGGCTGTGGTACACCTGGATCGTGGTCGCCATCGGTATCGTCGTCTTGTGGTGGGGCGTTCCCG
ACGGACCGCCGCGCGACTTCGAAGACGCGCCGGCGACCTGA

Upstream 100 bases:

>100_bases
GCGATCCTGCTGCCGTGGAGAGCCGCGCTGGACGCGCTTTCCAAGGCGTGACGCTCCGGCTAGGACCGCCGATCTGACGC
AACGGGGAGAGAGCGGGGCC

Downstream 100 bases:

>100_bases
CCGAGCGGTGGCGATCGAGTCTCCGCCCAGAACCCTGCGTCTCGATATCGATCGCGAGGCGCTGGAGGCCAATTGGCGGG
CACTCGATCGCTTGTCCGGC

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MRDAAASKGREPSENEIKLVIGASSAGTIFEWYDFFIYGTLAYILKDAFYDVDNETLGLLLVWSTFAVGFAFRPIGAVLF
GFLGDKLGRKYTFLVTVTLMGIATAGVGLIPTVATIGIAAPIIVILLRVIQGLALGGEYGGAAIYVAEHAPPEKRGFYTS
FIQASVAGGFVLSIAVVLACRFLIPEDDFVAWGWRVPFLLSIILLGISLWMRLKLSESPVFQAMKAEGQIAGNPFVESFT
YPGNKKRIFVALFGVTGILTTIWYTAFFSGMSFLRGPMHVADLTVELILFVSGLIAMTFYVMIGKWSDTIGRKKPIMIGA
VLTLLLLFPAFWGLGSLANPGLERAAAETPITVSGPQCTTDPFADLFDREQTDCGKVLETLTASGVPYTLEAGETLTLSA
GGQAIAIDPAWFEDGTARREGIRAALGEYGFDFSKQQPGMGNILGIVAILLGLGMLSALTYGSVAALLSEMFPPRIRYSS
MSIPYHIGAGYLGGFLPLIAGVIVASTGNIYSGLWYTWIVVAIGIVVLWWGVPDGPPRDFEDAPAT

Sequences:

>Translated_546_residues
MRDAAASKGREPSENEIKLVIGASSAGTIFEWYDFFIYGTLAYILKDAFYDVDNETLGLLLVWSTFAVGFAFRPIGAVLF
GFLGDKLGRKYTFLVTVTLMGIATAGVGLIPTVATIGIAAPIIVILLRVIQGLALGGEYGGAAIYVAEHAPPEKRGFYTS
FIQASVAGGFVLSIAVVLACRFLIPEDDFVAWGWRVPFLLSIILLGISLWMRLKLSESPVFQAMKAEGQIAGNPFVESFT
YPGNKKRIFVALFGVTGILTTIWYTAFFSGMSFLRGPMHVADLTVELILFVSGLIAMTFYVMIGKWSDTIGRKKPIMIGA
VLTLLLLFPAFWGLGSLANPGLERAAAETPITVSGPQCTTDPFADLFDREQTDCGKVLETLTASGVPYTLEAGETLTLSA
GGQAIAIDPAWFEDGTARREGIRAALGEYGFDFSKQQPGMGNILGIVAILLGLGMLSALTYGSVAALLSEMFPPRIRYSS
MSIPYHIGAGYLGGFLPLIAGVIVASTGNIYSGLWYTWIVVAIGIVVLWWGVPDGPPRDFEDAPAT
>Mature_546_residues
MRDAAASKGREPSENEIKLVIGASSAGTIFEWYDFFIYGTLAYILKDAFYDVDNETLGLLLVWSTFAVGFAFRPIGAVLF
GFLGDKLGRKYTFLVTVTLMGIATAGVGLIPTVATIGIAAPIIVILLRVIQGLALGGEYGGAAIYVAEHAPPEKRGFYTS
FIQASVAGGFVLSIAVVLACRFLIPEDDFVAWGWRVPFLLSIILLGISLWMRLKLSESPVFQAMKAEGQIAGNPFVESFT
YPGNKKRIFVALFGVTGILTTIWYTAFFSGMSFLRGPMHVADLTVELILFVSGLIAMTFYVMIGKWSDTIGRKKPIMIGA
VLTLLLLFPAFWGLGSLANPGLERAAAETPITVSGPQCTTDPFADLFDREQTDCGKVLETLTASGVPYTLEAGETLTLSA
GGQAIAIDPAWFEDGTARREGIRAALGEYGFDFSKQQPGMGNILGIVAILLGLGMLSALTYGSVAALLSEMFPPRIRYSS
MSIPYHIGAGYLGGFLPLIAGVIVASTGNIYSGLWYTWIVVAIGIVVLWWGVPDGPPRDFEDAPAT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=317, Percent_Identity=37.8548895899054, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1790550, Length=331, Percent_Identity=33.8368580060423, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789941, Length=324, Percent_Identity=37.037037037037, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788942, Length=336, Percent_Identity=32.4404761904762, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082404, Length=322, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082231, Length=292, Percent_Identity=23.6301369863014, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58605; Mature: 58605

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAAASKGREPSENEIKLVIGASSAGTIFEWYDFFIYGTLAYILKDAFYDVDNETLGLL
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LVWSTFAVGFAFRPIGAVLFGFLGDKLGRKYTFLVTVTLMGIATAGVGLIPTVATIGIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHH
PIIVILLRVIQGLALGGEYGGAAIYVAEHAPPEKRGFYTSFIQASVAGGFVLSIAVVLAC
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHH
RFLIPEDDFVAWGWRVPFLLSIILLGISLWMRLKLSESPVFQAMKAEGQIAGNPFVESFT
HHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCHHCCC
YPGNKKRIFVALFGVTGILTTIWYTAFFSGMSFLRGPMHVADLTVELILFVSGLIAMTFY
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VMIGKWSDTIGRKKPIMIGAVLTLLLLFPAFWGLGSLANPGLERAAAETPITVSGPQCTT
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCC
DPFADLFDREQTDCGKVLETLTASGVPYTLEAGETLTLSAGGQAIAIDPAWFEDGTARRE
CHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCHHHH
GIRAALGEYGFDFSKQQPGMGNILGIVAILLGLGMLSALTYGSVAALLSEMFPPRIRYSS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
MSIPYHIGAGYLGGFLPLIAGVIVASTGNIYSGLWYTWIVVAIGIVVLWWGVPDGPPRDF
CCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EDAPAT
CCCCCC
>Mature Secondary Structure
MRDAAASKGREPSENEIKLVIGASSAGTIFEWYDFFIYGTLAYILKDAFYDVDNETLGLL
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LVWSTFAVGFAFRPIGAVLFGFLGDKLGRKYTFLVTVTLMGIATAGVGLIPTVATIGIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHH
PIIVILLRVIQGLALGGEYGGAAIYVAEHAPPEKRGFYTSFIQASVAGGFVLSIAVVLAC
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHH
RFLIPEDDFVAWGWRVPFLLSIILLGISLWMRLKLSESPVFQAMKAEGQIAGNPFVESFT
HHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCHHCCC
YPGNKKRIFVALFGVTGILTTIWYTAFFSGMSFLRGPMHVADLTVELILFVSGLIAMTFY
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VMIGKWSDTIGRKKPIMIGAVLTLLLLFPAFWGLGSLANPGLERAAAETPITVSGPQCTT
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCC
DPFADLFDREQTDCGKVLETLTASGVPYTLEAGETLTLSAGGQAIAIDPAWFEDGTARRE
CHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCHHHH
GIRAALGEYGFDFSKQQPGMGNILGIVAILLGLGMLSALTYGSVAALLSEMFPPRIRYSS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
MSIPYHIGAGYLGGFLPLIAGVIVASTGNIYSGLWYTWIVVAIGIVVLWWGVPDGPPRDF
CCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EDAPAT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]