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The map label for this gene is aglA [H]

Identifier: 85373462

GI number: 85373462

Start: 666413

End: 668026

Strand: Direct

Name: aglA [H]

Synonym: ELI_03175

Alternate gene names: 85373462

Gene position: 666413-668026 (Clockwise)

Preceding gene: 85373461

Following gene: 85373463

Centisome position: 21.83

GC content: 63.26

Gene sequence:

>1614_bases
ATGGCAACTGCCAGCAACCCCTCCGCCGAGGCGGCAGGCGCGCTGCCATGGTGGCGCGGCGCGACGATCTACCAGATTTA
TCCGCGCAGCTTCATGGATGCCAATGGCGACGGTGTCGGCGACCTGCCGGGCATCACCCAGCGGCTCGACCACGTCGCCT
CGCTTGGCGTGGATGCGATCTGGATTTCGCCGTTCTTTAAATCGCCGATGAAGGACTTCGGCTACGACGTTTCCGACTAT
TGTGATGTCGATCCGCTGTTCGGCACGCTGCAGGATTTCGACGTGCTTATAGCCAAGGCGCACAGCCTCGGCCTGCGGGT
GCTGATCGACCAGGTCTATTCGCACACCTCCGACGAGCATCCCTGGTTCGCGAAAAGCCGCTCCAGCAAGGAAAGCGACA
AGGCGGACTGGTATGTCTGGGCCGATGCAAAGCCCGACGGGTCGCCGCCGAACAACTGGCAAAGCGTATTCGGCGGCCCG
GCGTGGACATGGGACGCGCGCCGCGGCCAGTATTACCTGCACAATTTCCTCAGCAGTCAGCCCAACATCAACGGCCACAA
TCCGCGCGTGCAGGAAGCGCTGCTCGATGTCGCGCGCTTCTGGCTCGATCGCGGGGTCGACGGTTTCCGGATCGACGCGC
TCAATTTCCTGATGTGCGATCCGGAATTGCGCGACAATCCGCCCGCACCGCCGAGCAACAAGCCGCGCACAAGGCCGTTC
GATTTCCAGATCAAGCAACATAATATGTCGCATCCGGCGATCCCCGATTTCGTCGCCCGCATCCGCGAGGTGACCGATGG
CTACGATGCGATCTTCACCGTGGCCGAAGTCGGCGGGGACGAAGCCGAAGGCGAGATGAAGGCGTTCACCGAAGGCGAAA
AACATCTCAACTCCGCCTATGGCTTCAACTTCCTCTATGCCGATAAGCTGACGCCCGGCCTCGTTTGTGGTGCCTTGGCA
CAATGGCCCGACGAAGACGGTGTCGGCTGGCCGAGCTGGGCTTTCGAAAACCACGATGCGCCGCGCGCGCTCTCGCGCTG
GTGCGCGCCCGAACACCGCGAGGCTTTTGCACGGCTCAAGATGGCGCTGCTGATGAGCCTGCGCGGCAATGCGATCCTCT
ATTACGGCGAGGAACTGGGGCTGACGCAGGTCGATATTCCCTTCGACCAGCTGCACGATCCCGAAGCGATCGCGAACTGG
CCGCTGACGCTCTCCCGCGATGGTGCGCGCACGCCGATGCCGTGGGAAGCGACGCAAGAATGCGCGGGTTTCGGTTCCGA
CGATACGTGGCTGCCGGTCGGGGTCGAGAACTTCGGCAAGGCCGTGGACAGGCAGGACGGGGACGATCGGTCGCTGCTCG
CCTTTACGCGGCGGATGATCGCGCTCCGCAAGGCCAATCCGGCGCTGCATCACGGTGCGGTGGAGAATTGCGGCCCGTAC
GGCAGCCTGCTCGACCTGACCCGCACCGCCGATGGCCAGCGGCTGCGTTGCCTGTTCAATCTCGGCCCCCAAACGCGCGA
ATTGACCGATGTCCCGGGCGTTGTCCTTCTCTCGGTCAATCAGGCTACCCCCGAAACCTTGCCGCCCTATGGCGCGCTGA
TCCTGGAGATCTGA

Upstream 100 bases:

>100_bases
GCGCCGCCGGGATCGCGGCGCCGGGCTCTGTCACGATGACGCTGCCTGCATTCGGCTATGCCGCGTGCGAGTTGACGGAC
GGGGAGGACGACTGAGGACC

Downstream 100 bases:

>100_bases
TCCGATGCGTTTGCTCCTTGCCGCCGCCTCTTCGCTGGCGCTCGCCACCCCGCTGACCGCAGCCGAAGTGTCGTCTCCCG
ATGGCCGGATCACTGTCGAA

Product: alpha-amylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MATASNPSAEAAGALPWWRGATIYQIYPRSFMDANGDGVGDLPGITQRLDHVASLGVDAIWISPFFKSPMKDFGYDVSDY
CDVDPLFGTLQDFDVLIAKAHSLGLRVLIDQVYSHTSDEHPWFAKSRSSKESDKADWYVWADAKPDGSPPNNWQSVFGGP
AWTWDARRGQYYLHNFLSSQPNINGHNPRVQEALLDVARFWLDRGVDGFRIDALNFLMCDPELRDNPPAPPSNKPRTRPF
DFQIKQHNMSHPAIPDFVARIREVTDGYDAIFTVAEVGGDEAEGEMKAFTEGEKHLNSAYGFNFLYADKLTPGLVCGALA
QWPDEDGVGWPSWAFENHDAPRALSRWCAPEHREAFARLKMALLMSLRGNAILYYGEELGLTQVDIPFDQLHDPEAIANW
PLTLSRDGARTPMPWEATQECAGFGSDDTWLPVGVENFGKAVDRQDGDDRSLLAFTRRMIALRKANPALHHGAVENCGPY
GSLLDLTRTADGQRLRCLFNLGPQTRELTDVPGVVLLSVNQATPETLPPYGALILEI

Sequences:

>Translated_537_residues
MATASNPSAEAAGALPWWRGATIYQIYPRSFMDANGDGVGDLPGITQRLDHVASLGVDAIWISPFFKSPMKDFGYDVSDY
CDVDPLFGTLQDFDVLIAKAHSLGLRVLIDQVYSHTSDEHPWFAKSRSSKESDKADWYVWADAKPDGSPPNNWQSVFGGP
AWTWDARRGQYYLHNFLSSQPNINGHNPRVQEALLDVARFWLDRGVDGFRIDALNFLMCDPELRDNPPAPPSNKPRTRPF
DFQIKQHNMSHPAIPDFVARIREVTDGYDAIFTVAEVGGDEAEGEMKAFTEGEKHLNSAYGFNFLYADKLTPGLVCGALA
QWPDEDGVGWPSWAFENHDAPRALSRWCAPEHREAFARLKMALLMSLRGNAILYYGEELGLTQVDIPFDQLHDPEAIANW
PLTLSRDGARTPMPWEATQECAGFGSDDTWLPVGVENFGKAVDRQDGDDRSLLAFTRRMIALRKANPALHHGAVENCGPY
GSLLDLTRTADGQRLRCLFNLGPQTRELTDVPGVVLLSVNQATPETLPPYGALILEI
>Mature_536_residues
ATASNPSAEAAGALPWWRGATIYQIYPRSFMDANGDGVGDLPGITQRLDHVASLGVDAIWISPFFKSPMKDFGYDVSDYC
DVDPLFGTLQDFDVLIAKAHSLGLRVLIDQVYSHTSDEHPWFAKSRSSKESDKADWYVWADAKPDGSPPNNWQSVFGGPA
WTWDARRGQYYLHNFLSSQPNINGHNPRVQEALLDVARFWLDRGVDGFRIDALNFLMCDPELRDNPPAPPSNKPRTRPFD
FQIKQHNMSHPAIPDFVARIREVTDGYDAIFTVAEVGGDEAEGEMKAFTEGEKHLNSAYGFNFLYADKLTPGLVCGALAQ
WPDEDGVGWPSWAFENHDAPRALSRWCAPEHREAFARLKMALLMSLRGNAILYYGEELGLTQVDIPFDQLHDPEAIANWP
LTLSRDGARTPMPWEATQECAGFGSDDTWLPVGVENFGKAVDRQDGDDRSLLAFTRRMIALRKANPALHHGAVENCGPYG
SLLDLTRTADGQRLRCLFNLGPQTRELTDVPGVVLLSVNQATPETLPPYGALILEI

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=511, Percent_Identity=31.8982387475538, Blast_Score=269, Evalue=6e-72,
Organism=Escherichia coli, GI1790687, Length=559, Percent_Identity=32.737030411449, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1786604, Length=110, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32565753, Length=372, Percent_Identity=28.494623655914, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25147709, Length=388, Percent_Identity=26.8041237113402, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322245, Length=548, Percent_Identity=33.3941605839416, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6321731, Length=527, Percent_Identity=35.2941176470588, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319776, Length=527, Percent_Identity=35.2941176470588, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6322241, Length=558, Percent_Identity=33.8709677419355, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322021, Length=558, Percent_Identity=33.8709677419355, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321726, Length=513, Percent_Identity=35.0877192982456, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324416, Length=558, Percent_Identity=33.8709677419355, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24586587, Length=526, Percent_Identity=36.1216730038023, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24583745, Length=486, Percent_Identity=37.8600823045267, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24586591, Length=519, Percent_Identity=35.8381502890173, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24586589, Length=502, Percent_Identity=37.6494023904382, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45549022, Length=528, Percent_Identity=34.8484848484849, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24586599, Length=510, Percent_Identity=35.6862745098039, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24583749, Length=536, Percent_Identity=34.5149253731343, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24583747, Length=536, Percent_Identity=34.5149253731343, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24586597, Length=488, Percent_Identity=35.4508196721311, Blast_Score=278, Evalue=7e-75,
Organism=Drosophila melanogaster, GI24586593, Length=498, Percent_Identity=34.9397590361446, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI221330053, Length=573, Percent_Identity=32.6352530541012, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281360393, Length=490, Percent_Identity=30.8163265306122, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 59736; Mature: 59605

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATASNPSAEAAGALPWWRGATIYQIYPRSFMDANGDGVGDLPGITQRLDHVASLGVDAI
CCCCCCCCCHHCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
WISPFFKSPMKDFGYDVSDYCDVDPLFGTLQDFDVLIAKAHSLGLRVLIDQVYSHTSDEH
EECHHHHCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWFAKSRSSKESDKADWYVWADAKPDGSPPNNWQSVFGGPAWTWDARRGQYYLHNFLSSQ
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHCCC
PNINGHNPRVQEALLDVARFWLDRGVDGFRIDALNFLMCDPELRDNPPAPPSNKPRTRPF
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCC
DFQIKQHNMSHPAIPDFVARIREVTDGYDAIFTVAEVGGDEAEGEMKAFTEGEKHLNSAY
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHC
GFNFLYADKLTPGLVCGALAQWPDEDGVGWPSWAFENHDAPRALSRWCAPEHREAFARLK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHH
MALLMSLRGNAILYYGEELGLTQVDIPFDQLHDPEAIANWPLTLSRDGARTPMPWEATQE
HHHHHHHCCCEEEEECCCCCCEEECCCHHHCCCCHHHHCCCEEECCCCCCCCCCCHHHHH
CAGFGSDDTWLPVGVENFGKAVDRQDGDDRSLLAFTRRMIALRKANPALHHGAVENCGPY
HHCCCCCCCEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCHHCCCCC
GSLLDLTRTADGQRLRCLFNLGPQTRELTDVPGVVLLSVNQATPETLPPYGALILEI
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
ATASNPSAEAAGALPWWRGATIYQIYPRSFMDANGDGVGDLPGITQRLDHVASLGVDAI
CCCCCCCCHHCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
WISPFFKSPMKDFGYDVSDYCDVDPLFGTLQDFDVLIAKAHSLGLRVLIDQVYSHTSDEH
EECHHHHCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWFAKSRSSKESDKADWYVWADAKPDGSPPNNWQSVFGGPAWTWDARRGQYYLHNFLSSQ
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHCCC
PNINGHNPRVQEALLDVARFWLDRGVDGFRIDALNFLMCDPELRDNPPAPPSNKPRTRPF
CCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCC
DFQIKQHNMSHPAIPDFVARIREVTDGYDAIFTVAEVGGDEAEGEMKAFTEGEKHLNSAY
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHC
GFNFLYADKLTPGLVCGALAQWPDEDGVGWPSWAFENHDAPRALSRWCAPEHREAFARLK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHH
MALLMSLRGNAILYYGEELGLTQVDIPFDQLHDPEAIANWPLTLSRDGARTPMPWEATQE
HHHHHHHCCCEEEEECCCCCCEEECCCHHHCCCCHHHHCCCEEECCCCCCCCCCCHHHHH
CAGFGSDDTWLPVGVENFGKAVDRQDGDDRSLLAFTRRMIALRKANPALHHGAVENCGPY
HHCCCCCCCEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCHHCCCCC
GSLLDLTRTADGQRLRCLFNLGPQTRELTDVPGVVLLSVNQATPETLPPYGALILEI
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]