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The map label for this gene is amy [H]

Identifier: 85373461

GI number: 85373461

Start: 664593

End: 666407

Strand: Direct

Name: amy [H]

Synonym: ELI_03170

Alternate gene names: 85373461

Gene position: 664593-666407 (Clockwise)

Preceding gene: 85373460

Following gene: 85373462

Centisome position: 21.77

GC content: 62.04

Gene sequence:

>1815_bases
TTGGCGCTCGCCGCCAGCACCACTTCGCCAACGCTCGCGCAGGAACCCTCCGCCGAAACGCTCGCCAGCGTCCGCGATCG
TGCGCCGACCGATGAAGTGATCTACTTCGTCCTGCCAGATCGCTTCGCCAATGGCGATGCCTCGAACGACTTGGGCGGCT
ACGAAGCGGACCGGCTCAAGAGCGGTTACGACCCGACCCACAAGGGCTTCTATCATGGCGGCGATCTCAAAGGCCTGACC
GAAAAGCTCGACTATATCCAGGGCATGGGCGTGACCGCGATCTGGTTCGCACCGATCTTCAAGAACAAGCCGGTGCAGGG
CGATTTCTCCAAGCCTGAAACCGTCAGCGCGGGCTACCACGGATACTGGGTCACCGACTTCACGCAAATCGATCCGCATT
TCGGTACCAATGCCGAGTTCGAGGCCTTCGTCGACGCCGCCCATGCACGCGGGATGAAGGTCTATATGGACATCATCACC
AACCACACCGCCGACGTGATCAATTATGCGGAGGGCGAATACGCCTATCGCAGCAAGGGGGCTTACCCGTATTCGACCCG
CTACGGGCCGGACGGCGAGCGGATAAATCCGGGCTTCAACGGCGACCAGGATTCGAGTGAGGCCAATTGGGCCAGACTGG
TGAACCCGGATTACGCCTATTCACCGGTCGTTTCCGAGGCGGAGAGGGACATCAAGGTCCCGATCTGGCTCAACGATCCG
ATCTATTACCACAATCGCGGTGACAGCGAGTGGTGGGGCGAGAGCGCGCTCTATGGCGATTTCGCCGGGCTCGACGATCT
CTTCACCGAGCACCCGCGAGTGGTCGAGGGCATGATCGAGATCTTCGGCAGCTGGATCGACCGCTTCGGCATCGACGGCT
TTCGCATCGATACCGTCAAGCATGTAAACCCCCAATTCTGGCAGGCTTTCGTGCCCGCCATGCTCGAACGCGCGGAAGCT
GCGGGCATTCCCAATTTCCATATCTTCGGCGAAGTCTATTGGGCCGGGCCGCAACCGGGCGGGCTGCATCAATATACGAT
CCGTGACCGGATGCCGGCGGTGCTGGATTTCACCTTCCAGGCGGCGGTACAGGAAGTGGTCGCGAAAAGCGCCAGCCCCG
AAGTGCTCTATGAAATGCTGCGCGGCGATATCCTCTACGCGCAAGGTGAAGAGACCGCGCTGCAGAACCCGACCTTCCTC
GGCAACCACGATATGGGCCGCTTTTCGATGTTCGTGAAACAGGCCAATCCGGATGCCGACAACGACGAGCTCCTCAAGCG
CGTGATGCTCGGCAATGCCATGATGCTGACCCTGCGCGGCGTGCCGACGATCTATTACGGCGACGAGCAGGGCTTCGTGT
CCGACGGCAACGACCAGCTGGCACGCGAGAACATGTTCCCGAGCCGGGTCGATGCCTACAACGACAACGACCTGCTCGGC
ACCGACGCAACGACCGCGGGCGACAATTACGATACGCAGCATCCGCTCTATCGCCTGATCAAGGGGTTGTCGGCGGTGCG
CCTCGCCAATCCGGCGCTCAAGACCGGGCGGCACACGCTCGGCACCTTCGATCGCGAGCCGGGGCTGTTCTCGCTCACGC
GCACCGATCCGGAGAGCGGCCAGCGCGTGCTGTTGGCCTTCAACACCAGCAGGGAAGAGATCACGCGCGATATCGAGATC
GCTTACGACGTGACCGGTGTGCAGGCGCTGGTCGGACCTTGCGCCGCCGGGATCGCGGCGCCGGGCTCTGTCACGATGAC
GCTGCCTGCATTCGGCTATGCCGCGTGCGAGTTGACGGACGGGGAGGACGACTGA

Upstream 100 bases:

>100_bases
ATCATGCGCCTTTCCTGCGCGCGCATATCGACCCGCGAAAGCAGGCCCCCACACAGCAGAGGATTCCCGCCTGATGCGTA
TCGCCCTAGCAGCTTTAGCC

Downstream 100 bases:

>100_bases
GGACCATGGCAACTGCCAGCAACCCCTCCGCCGAGGCGGCAGGCGCGCTGCCATGGTGGCGCGGCGCGACGATCTACCAG
ATTTATCCGCGCAGCTTCAT

Product: alpha-amylase family protein

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MALAASTTSPTLAQEPSAETLASVRDRAPTDEVIYFVLPDRFANGDASNDLGGYEADRLKSGYDPTHKGFYHGGDLKGLT
EKLDYIQGMGVTAIWFAPIFKNKPVQGDFSKPETVSAGYHGYWVTDFTQIDPHFGTNAEFEAFVDAAHARGMKVYMDIIT
NHTADVINYAEGEYAYRSKGAYPYSTRYGPDGERINPGFNGDQDSSEANWARLVNPDYAYSPVVSEAERDIKVPIWLNDP
IYYHNRGDSEWWGESALYGDFAGLDDLFTEHPRVVEGMIEIFGSWIDRFGIDGFRIDTVKHVNPQFWQAFVPAMLERAEA
AGIPNFHIFGEVYWAGPQPGGLHQYTIRDRMPAVLDFTFQAAVQEVVAKSASPEVLYEMLRGDILYAQGEETALQNPTFL
GNHDMGRFSMFVKQANPDADNDELLKRVMLGNAMMLTLRGVPTIYYGDEQGFVSDGNDQLARENMFPSRVDAYNDNDLLG
TDATTAGDNYDTQHPLYRLIKGLSAVRLANPALKTGRHTLGTFDREPGLFSLTRTDPESGQRVLLAFNTSREEITRDIEI
AYDVTGVQALVGPCAAGIAAPGSVTMTLPAFGYAACELTDGEDD

Sequences:

>Translated_604_residues
MALAASTTSPTLAQEPSAETLASVRDRAPTDEVIYFVLPDRFANGDASNDLGGYEADRLKSGYDPTHKGFYHGGDLKGLT
EKLDYIQGMGVTAIWFAPIFKNKPVQGDFSKPETVSAGYHGYWVTDFTQIDPHFGTNAEFEAFVDAAHARGMKVYMDIIT
NHTADVINYAEGEYAYRSKGAYPYSTRYGPDGERINPGFNGDQDSSEANWARLVNPDYAYSPVVSEAERDIKVPIWLNDP
IYYHNRGDSEWWGESALYGDFAGLDDLFTEHPRVVEGMIEIFGSWIDRFGIDGFRIDTVKHVNPQFWQAFVPAMLERAEA
AGIPNFHIFGEVYWAGPQPGGLHQYTIRDRMPAVLDFTFQAAVQEVVAKSASPEVLYEMLRGDILYAQGEETALQNPTFL
GNHDMGRFSMFVKQANPDADNDELLKRVMLGNAMMLTLRGVPTIYYGDEQGFVSDGNDQLARENMFPSRVDAYNDNDLLG
TDATTAGDNYDTQHPLYRLIKGLSAVRLANPALKTGRHTLGTFDREPGLFSLTRTDPESGQRVLLAFNTSREEITRDIEI
AYDVTGVQALVGPCAAGIAAPGSVTMTLPAFGYAACELTDGEDD
>Mature_603_residues
ALAASTTSPTLAQEPSAETLASVRDRAPTDEVIYFVLPDRFANGDASNDLGGYEADRLKSGYDPTHKGFYHGGDLKGLTE
KLDYIQGMGVTAIWFAPIFKNKPVQGDFSKPETVSAGYHGYWVTDFTQIDPHFGTNAEFEAFVDAAHARGMKVYMDIITN
HTADVINYAEGEYAYRSKGAYPYSTRYGPDGERINPGFNGDQDSSEANWARLVNPDYAYSPVVSEAERDIKVPIWLNDPI
YYHNRGDSEWWGESALYGDFAGLDDLFTEHPRVVEGMIEIFGSWIDRFGIDGFRIDTVKHVNPQFWQAFVPAMLERAEAA
GIPNFHIFGEVYWAGPQPGGLHQYTIRDRMPAVLDFTFQAAVQEVVAKSASPEVLYEMLRGDILYAQGEETALQNPTFLG
NHDMGRFSMFVKQANPDADNDELLKRVMLGNAMMLTLRGVPTIYYGDEQGFVSDGNDQLARENMFPSRVDAYNDNDLLGT
DATTAGDNYDTQHPLYRLIKGLSAVRLANPALKTGRHTLGTFDREPGLFSLTRTDPESGQRVLLAFNTSREEITRDIEIA
YDVTGVQALVGPCAAGIAAPGSVTMTLPAFGYAACELTDGEDD

Specific function: Since Only Maltooligosaccharides Up To A Chain Length Of 6 Glucose Units Are Actively Transported Through The Cytoplasmic Membrane VIa The Membrane-Bound Complex Of Three Proteins, Malf, Malg, And Malk, Longer Maltooligosaccharides Must First Be Degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=233, Percent_Identity=26.6094420600858, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1789995, Length=572, Percent_Identity=25.6993006993007, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1790687, Length=463, Percent_Identity=25.2699784017279, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786604, Length=147, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=92, Percent_Identity=36.9565217391304, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=91, Percent_Identity=36.2637362637363, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321731, Length=91, Percent_Identity=36.2637362637363, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24586589, Length=237, Percent_Identity=30.8016877637131, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24586587, Length=229, Percent_Identity=28.82096069869, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583747, Length=581, Percent_Identity=23.2358003442341, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583749, Length=581, Percent_Identity=23.2358003442341, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586591, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24586593, Length=234, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586597, Length=230, Percent_Identity=29.5652173913043, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45549022, Length=230, Percent_Identity=29.1304347826087, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221330053, Length=92, Percent_Identity=36.9565217391304, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586599, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583745, Length=92, Percent_Identity=33.695652173913, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 66800; Mature: 66669

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAASTTSPTLAQEPSAETLASVRDRAPTDEVIYFVLPDRFANGDASNDLGGYEADRLK
CCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHC
SGYDPTHKGFYHGGDLKGLTEKLDYIQGMGVTAIWFAPIFKNKPVQGDFSKPETVSAGYH
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCEECCCCC
GYWVTDFTQIDPHFGTNAEFEAFVDAAHARGMKVYMDIITNHTADVINYAEGEYAYRSKG
CEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHCCHHHHHHCCCCCCHHHCCC
AYPYSTRYGPDGERINPGFNGDQDSSEANWARLVNPDYAYSPVVSEAERDIKVPIWLNDP
CCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCEEEEEEECCC
IYYHNRGDSEWWGESALYGDFAGLDDLFTEHPRVVEGMIEIFGSWIDRFGIDGFRIDTVK
EEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
HVNPQFWQAFVPAMLERAEAAGIPNFHIFGEVYWAGPQPGGLHQYTIRDRMPAVLDFTFQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHH
AAVQEVVAKSASPEVLYEMLRGDILYAQGEETALQNPTFLGNHDMGRFSMFVKQANPDAD
HHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCHHHHEEECCCCCCC
NDELLKRVMLGNAMMLTLRGVPTIYYGDEQGFVSDGNDQLARENMFPSRVDAYNDNDLLG
HHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHCCCCCHHCCCCCCCCCC
TDATTAGDNYDTQHPLYRLIKGLSAVRLANPALKTGRHTLGTFDREPGLFSLTRTDPESG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCC
QRVLLAFNTSREEITRDIEIAYDVTGVQALVGPCAAGIAAPGSVTMTLPAFGYAACELTD
CEEEEEECCCHHHHHCCEEEEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECC
GEDD
CCCC
>Mature Secondary Structure 
ALAASTTSPTLAQEPSAETLASVRDRAPTDEVIYFVLPDRFANGDASNDLGGYEADRLK
CEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHC
SGYDPTHKGFYHGGDLKGLTEKLDYIQGMGVTAIWFAPIFKNKPVQGDFSKPETVSAGYH
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCCCEECCCCC
GYWVTDFTQIDPHFGTNAEFEAFVDAAHARGMKVYMDIITNHTADVINYAEGEYAYRSKG
CEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEHHHHCCHHHHHHCCCCCCHHHCCC
AYPYSTRYGPDGERINPGFNGDQDSSEANWARLVNPDYAYSPVVSEAERDIKVPIWLNDP
CCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCEEEEEEECCC
IYYHNRGDSEWWGESALYGDFAGLDDLFTEHPRVVEGMIEIFGSWIDRFGIDGFRIDTVK
EEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
HVNPQFWQAFVPAMLERAEAAGIPNFHIFGEVYWAGPQPGGLHQYTIRDRMPAVLDFTFQ
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHH
AAVQEVVAKSASPEVLYEMLRGDILYAQGEETALQNPTFLGNHDMGRFSMFVKQANPDAD
HHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCHHHHEEECCCCCCC
NDELLKRVMLGNAMMLTLRGVPTIYYGDEQGFVSDGNDQLARENMFPSRVDAYNDNDLLG
HHHHHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHCCCCCHHCCCCCCCCCC
TDATTAGDNYDTQHPLYRLIKGLSAVRLANPALKTGRHTLGTFDREPGLFSLTRTDPESG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCC
QRVLLAFNTSREEITRDIEIAYDVTGVQALVGPCAAGIAAPGSVTMTLPAFGYAACELTD
CEEEEEECCCHHHHHCCEEEEEECHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECC
GEDD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]