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The map label for this gene is hsaD [H]

Identifier: 85373301

GI number: 85373301

Start: 513553

End: 514524

Strand: Direct

Name: hsaD [H]

Synonym: ELI_02370

Alternate gene names: 85373301

Gene position: 513553-514524 (Clockwise)

Preceding gene: 85373300

Following gene: 85373302

Centisome position: 16.82

GC content: 65.02

Gene sequence:

>972_bases
ATGAAGCGACTGCTGAAGATCCTCGCGGTGATCGTCGCGCTGCTGGTCATCGCATTCTTCATCCTGCGCACGCCCGATAC
CGATCCGGAAGAGATGCGGGCGAAATACGGCACCGCGCCGTCGCAATTCGTCACGCTCGCCAACGGGCAGGAACTGCATC
TGCGCGACGAGGGGCCGCGCGACGCGATGCCGATCGTGCTGCTGCACGGCTCCAACGCCGATATGTCGACCTGGCAGCCA
TGGGCAGAGGCGCTGCGCAATGATTATCGCGTGATCCGCTTCGACCAGATCGGCCACGGTCTGACCGGCGCGGCCTTCGA
CGAGGATTATTCGGGCGACCGTTTCATCGAAAGCGTGCATCTGGTGGCCGACAGGCTGGACCTCGACCGGTTCGTGCTGG
CGGGCAATTCGATGGGCGGCGGGATCGCCATGGGCTTCGCCATGCGCCATCCGGAGCGGCTTTCGGGCCTCGTGCTGGTC
GATGCCGGCGGTGCCCCGATCCGGCGCGAGGGCGGGGGCAATCTCGCCTTCACGCTGGCGCAGATGCCGGTGGTCGGCAA
CGTGATGAGTGCAATGCTGCCCCGCTCGATCGTCGAGCGCAGCCTGAAGCAAAGCGTCAGCAAGACCGAAATCGTCGATG
ACGCCATGGTCGATCGCTATTTCGACATGGCACGCTATCCCGGCAATCGCGCCGCCACCCGCGCACGTTTCTCGCAGGAG
CGGGTGCCCTTCGAAGCCGAGCAGGTAGCCAGGGTCACTGTCCCGACGCTGGTCATGTGGGGCGAGGAAGACAGCCTGAT
CCCCTATACCGCCGCCGGATGGTTCATGGAGCACCTGCCCAATGCGACGCTCGCCAACTATCCGGGGATAGGTCATCTGC
CGCAGGAGGAAGCGCCCGAGCGGTCGGTGGCCGACCTGAAGGCATGGCTCGCAGGTATAGCGGGCAACGAACCAGCGATC
GAGCCCGCTTAG

Upstream 100 bases:

>100_bases
GCACCGAGCCCGGCGTGGCGATGCCGGAACTGGGCAAGTCGACCGTCGATGAAGAGGCGCTGGAAGTGATCGAGCGCTGG
ATCGCGCAGATGGAAGCACC

Downstream 100 bases:

>100_bases
AGGCTGGCGGATAACCCGCTCACGCGCTTGCGAAACTTGGCGCCGCCTCTAGAAACGGCCGGAACACGCTCTCGTTTGGA
GCGAGGCCATTGGGGGTGAT

Product: hydrolase, alpha/beta hydrolase fold family protein

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKRLLKILAVIVALLVIAFFILRTPDTDPEEMRAKYGTAPSQFVTLANGQELHLRDEGPRDAMPIVLLHGSNADMSTWQP
WAEALRNDYRVIRFDQIGHGLTGAAFDEDYSGDRFIESVHLVADRLDLDRFVLAGNSMGGGIAMGFAMRHPERLSGLVLV
DAGGAPIRREGGGNLAFTLAQMPVVGNVMSAMLPRSIVERSLKQSVSKTEIVDDAMVDRYFDMARYPGNRAATRARFSQE
RVPFEAEQVARVTVPTLVMWGEEDSLIPYTAAGWFMEHLPNATLANYPGIGHLPQEEAPERSVADLKAWLAGIAGNEPAI
EPA

Sequences:

>Translated_323_residues
MKRLLKILAVIVALLVIAFFILRTPDTDPEEMRAKYGTAPSQFVTLANGQELHLRDEGPRDAMPIVLLHGSNADMSTWQP
WAEALRNDYRVIRFDQIGHGLTGAAFDEDYSGDRFIESVHLVADRLDLDRFVLAGNSMGGGIAMGFAMRHPERLSGLVLV
DAGGAPIRREGGGNLAFTLAQMPVVGNVMSAMLPRSIVERSLKQSVSKTEIVDDAMVDRYFDMARYPGNRAATRARFSQE
RVPFEAEQVARVTVPTLVMWGEEDSLIPYTAAGWFMEHLPNATLANYPGIGHLPQEEAPERSVADLKAWLAGIAGNEPAI
EPA
>Mature_323_residues
MKRLLKILAVIVALLVIAFFILRTPDTDPEEMRAKYGTAPSQFVTLANGQELHLRDEGPRDAMPIVLLHGSNADMSTWQP
WAEALRNDYRVIRFDQIGHGLTGAAFDEDYSGDRFIESVHLVADRLDLDRFVLAGNSMGGGIAMGFAMRHPERLSGLVLV
DAGGAPIRREGGGNLAFTLAQMPVVGNVMSAMLPRSIVERSLKQSVSKTEIVDDAMVDRYFDMARYPGNRAATRARFSQE
RVPFEAEQVARVTVPTLVMWGEEDSLIPYTAAGWFMEHLPNATLANYPGIGHLPQEEAPERSVADLKAWLAGIAGNEPAI
EPA

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Al

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=272, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 35485; Mature: 35485

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLKILAVIVALLVIAFFILRTPDTDPEEMRAKYGTAPSQFVTLANGQELHLRDEGPR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHEEEECCCCEEEECCCCCC
DAMPIVLLHGSNADMSTWQPWAEALRNDYRVIRFDQIGHGLTGAAFDEDYSGDRFIESVH
CCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
LVADRLDLDRFVLAGNSMGGGIAMGFAMRHPERLSGLVLVDAGGAPIRREGGGNLAFTLA
HHHHHHCCCCEEEECCCCCCHHHHHHHHCCHHHHCCEEEEECCCCCEEECCCCCEEEEEE
QMPVVGNVMSAMLPRSIVERSLKQSVSKTEIVDDAMVDRYFDMARYPGNRAATRARFSQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RVPFEAEQVARVTVPTLVMWGEEDSLIPYTAAGWFMEHLPNATLANYPGIGHLPQEEAPE
CCCCCHHHHHHEEECEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RSVADLKAWLAGIAGNEPAIEPA
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRLLKILAVIVALLVIAFFILRTPDTDPEEMRAKYGTAPSQFVTLANGQELHLRDEGPR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHEEEECCCCEEEECCCCCC
DAMPIVLLHGSNADMSTWQPWAEALRNDYRVIRFDQIGHGLTGAAFDEDYSGDRFIESVH
CCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
LVADRLDLDRFVLAGNSMGGGIAMGFAMRHPERLSGLVLVDAGGAPIRREGGGNLAFTLA
HHHHHHCCCCEEEECCCCCCHHHHHHHHCCHHHHCCEEEEECCCCCEEECCCCCEEEEEE
QMPVVGNVMSAMLPRSIVERSLKQSVSKTEIVDDAMVDRYFDMARYPGNRAATRARFSQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RVPFEAEQVARVTVPTLVMWGEEDSLIPYTAAGWFMEHLPNATLANYPGIGHLPQEEAPE
CCCCCHHHHHHEEECEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RSVADLKAWLAGIAGNEPAIEPA
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA