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The map label for this gene is ccrM [H]

Identifier: 85373286

GI number: 85373286

Start: 498544

End: 499686

Strand: Direct

Name: ccrM [H]

Synonym: ELI_02295

Alternate gene names: 85373286

Gene position: 498544-499686 (Clockwise)

Preceding gene: 85373285

Following gene: 85373288

Centisome position: 16.33

GC content: 64.48

Gene sequence:

>1143_bases
ATGATGGGGGTCGTGGAGACCACGAAACAGCGCGTGCGCCGTGCGAGCAAGCTGGTCGCAGTGCCGAAGGAAACGCTCCC
GTTGGGGCAGATCCTCTCCGGCGATTGCGTCGAGGCCATGCGCTCACTGCCCGACGCCAGCGTCGATCTCGTCTTCGCCG
ATCCGCCTTACAACCTGCAACTCGGCGGCGATCTCAACCGCCCCGATGGTAGCCATGTCGATGCCGTCACCGATCATTGG
GACCAGTTCGACAGCTTTGCCATCTACGACCAATTCACCCGCGAGTGGCTGACCGAGGCCAAGCGCATCCTCAAGCCCGA
CGGCGCGCTGTGGGTGATCGGCAGCTATCACAACATCTACCGCGTCGGCGCGATCCTGCAGGATTTGGGCTTCTGGATCC
TCAATGACATCGTCTGGCGCAAGACCAACCCGATGCCCAATTTCCGCGGCACCCGCTTCACCAATGCGCATGAGACGCTG
CTGTGGGCGAGCCAGGGCGAGAAGGCGAAGTACCACTTCAACTACACCGCCATGAAGACGCTCAATGACGAGCTGCAGAT
GCGCAGCGACTGGGTGATCCCGATCTGCAACGGGGCCGAGCGGCTCAAGGAAAACGGCACCAAGGCGCACCCGACGCAAA
AGCCCGAGGCGCTGCTCTATCGCGTGCTGCTGGCGACGACGGAGAAGGGCGACGTGGTGGTCGACCCGTTCTTCGGCACC
GGCACCACCGGCGCGGTTGCCAAGCGGCTGGGCCGCGAGTGGATCGGCTGCGAGCGCGAGGATGTCTATCGCGGCGTGGC
GATGAAGCGGATCGAGAAAGAACTGCCGCTCGACGAAAGCGCGCTCAAGGTGATGCAATCGAAGCGCGCCGCGCCCAAGG
TGGCCTTCGGCGCGCTTGTGGAGGCAGGGTTCCTCAAGCCCGGCACCGAAGTGTTCGACACAAAACGCCGCTGGGTCGCG
ACGGTGCGCGCGGATGGCTCGCTGGAATGCGGGAAGCAGACCGGCTCAATCCACGGCCTCGGCAAGGAGCTGCAAGGCGC
CCCCAGCTGCAACGGCTGGACCTTCTGGCATTACGAGGATGAGGGCGTGGCGAAGCCGCTGGACGCCGCGCGGCAGCTGT
ATCTGCTGGCGGTGGAGGATTGA

Upstream 100 bases:

>100_bases
GCTGAGGTTGACGCGACGTTAACCATAGTCTGCGAAACTTCGGTCTTGACGCGGAGTCCGCTTGGACTCACCCTGTGGAT
AACTGGAGTGACGGGGGAAG

Downstream 100 bases:

>100_bases
ATTGAACTTGGCAGATTAAAGGCTCCTTATGGGTGGTTTGCTGACAGGCCGCATTTGTTGCGAAGAACTCCGCGAGTAGC
CTGTCCGGTTTCAAGATATA

Product: modification methylase

Products: NA

Alternate protein names: M.BabI; Adenine-specific methyltransferase BabI; DNA methyltransferase CcrM; M.CcrMI ortholog [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MMGVVETTKQRVRRASKLVAVPKETLPLGQILSGDCVEAMRSLPDASVDLVFADPPYNLQLGGDLNRPDGSHVDAVTDHW
DQFDSFAIYDQFTREWLTEAKRILKPDGALWVIGSYHNIYRVGAILQDLGFWILNDIVWRKTNPMPNFRGTRFTNAHETL
LWASQGEKAKYHFNYTAMKTLNDELQMRSDWVIPICNGAERLKENGTKAHPTQKPEALLYRVLLATTEKGDVVVDPFFGT
GTTGAVAKRLGREWIGCEREDVYRGVAMKRIEKELPLDESALKVMQSKRAAPKVAFGALVEAGFLKPGTEVFDTKRRWVA
TVRADGSLECGKQTGSIHGLGKELQGAPSCNGWTFWHYEDEGVAKPLDAARQLYLLAVED

Sequences:

>Translated_380_residues
MMGVVETTKQRVRRASKLVAVPKETLPLGQILSGDCVEAMRSLPDASVDLVFADPPYNLQLGGDLNRPDGSHVDAVTDHW
DQFDSFAIYDQFTREWLTEAKRILKPDGALWVIGSYHNIYRVGAILQDLGFWILNDIVWRKTNPMPNFRGTRFTNAHETL
LWASQGEKAKYHFNYTAMKTLNDELQMRSDWVIPICNGAERLKENGTKAHPTQKPEALLYRVLLATTEKGDVVVDPFFGT
GTTGAVAKRLGREWIGCEREDVYRGVAMKRIEKELPLDESALKVMQSKRAAPKVAFGALVEAGFLKPGTEVFDTKRRWVA
TVRADGSLECGKQTGSIHGLGKELQGAPSCNGWTFWHYEDEGVAKPLDAARQLYLLAVED
>Mature_380_residues
MMGVVETTKQRVRRASKLVAVPKETLPLGQILSGDCVEAMRSLPDASVDLVFADPPYNLQLGGDLNRPDGSHVDAVTDHW
DQFDSFAIYDQFTREWLTEAKRILKPDGALWVIGSYHNIYRVGAILQDLGFWILNDIVWRKTNPMPNFRGTRFTNAHETL
LWASQGEKAKYHFNYTAMKTLNDELQMRSDWVIPICNGAERLKENGTKAHPTQKPEALLYRVLLATTEKGDVVVDPFFGT
GTTGAVAKRLGREWIGCEREDVYRGVAMKRIEKELPLDESALKVMQSKRAAPKVAFGALVEAGFLKPGTEVFDTKRRWVA
TVRADGSLECGKQTGSIHGLGKELQGAPSCNGWTFWHYEDEGVAKPLDAARQLYLLAVED

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=262, Percent_Identity=29.3893129770992, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42555; Mature: 42555

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGVVETTKQRVRRASKLVAVPKETLPLGQILSGDCVEAMRSLPDASVDLVFADPPYNLQ
CCCCHHHHHHHHHHHHHHEECCHHHCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEEE
LGGDLNRPDGSHVDAVTDHWDQFDSFAIYDQFTREWLTEAKRILKPDGALWVIGSYHNIY
ECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
RVGAILQDLGFWILNDIVWRKTNPMPNFRGTRFTNAHETLLWASQGEKAKYHFNYTAMKT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHEEEEECCCCCEEEEEEEHHHHH
LNDELQMRSDWVIPICNGAERLKENGTKAHPTQKPEALLYRVLLATTEKGDVVVDPFFGT
HHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
GTTGAVAKRLGREWIGCEREDVYRGVAMKRIEKELPLDESALKVMQSKRAAPKVAFGALV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
EAGFLKPGTEVFDTKRRWVATVRADGSLECGKQTGSIHGLGKELQGAPSCNGWTFWHYED
HHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECC
EGVAKPLDAARQLYLLAVED
CCCCCHHHHCCEEEEEEECC
>Mature Secondary Structure
MMGVVETTKQRVRRASKLVAVPKETLPLGQILSGDCVEAMRSLPDASVDLVFADPPYNLQ
CCCCHHHHHHHHHHHHHHEECCHHHCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEEE
LGGDLNRPDGSHVDAVTDHWDQFDSFAIYDQFTREWLTEAKRILKPDGALWVIGSYHNIY
ECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
RVGAILQDLGFWILNDIVWRKTNPMPNFRGTRFTNAHETLLWASQGEKAKYHFNYTAMKT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHEEEEECCCCCEEEEEEEHHHHH
LNDELQMRSDWVIPICNGAERLKENGTKAHPTQKPEALLYRVLLATTEKGDVVVDPFFGT
HHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
GTTGAVAKRLGREWIGCEREDVYRGVAMKRIEKELPLDESALKVMQSKRAAPKVAFGALV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
EAGFLKPGTEVFDTKRRWVATVRADGSLECGKQTGSIHGLGKELQGAPSCNGWTFWHYED
HHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECC
EGVAKPLDAARQLYLLAVED
CCCCCHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]