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The map label for this gene is proB [H]

Identifier: 85373199

GI number: 85373199

Start: 418199

End: 419317

Strand: Direct

Name: proB [H]

Synonym: ELI_01860

Alternate gene names: 85373199

Gene position: 418199-419317 (Clockwise)

Preceding gene: 85373198

Following gene: 85373200

Centisome position: 13.7

GC content: 68.63

Gene sequence:

>1119_bases
GTGGTCGCCGATCTGAGCGATCTTGCCCGATCCCAGCGTCTTGTGGTCAAGGTTGGCAGCTCGCTGCTGGTCGAGCACGG
CGAGGCGCGCGCGGATTGGCTGAAATCGCTGGCGCAGGACCTCGCCCAGCTTCGTGCGAAAGGCACGAAGGTCATCGTCG
TCAGTTCGGGCGCCATTGCGCTCGGCGCGGCGCGGCTGGGCTTCGAGAAGGGCGGACGCGGCAGCCTGTCGGATGCTCAG
GCGAGCGCATCGGTCGGGCAGATTGCGCTGGCCGGGCTCTGGTCGCAGGCACTCGGCGATGTCGGGCTGACCGCAGCGCA
GATCCTCCTTTCGCTCGATGATCTCGAAGACCGCGCGCGCTATCTCAACGCCTCGGCTACCCTGGGGCGATTGCTGGATG
CCGGTGCAGTGCCGGTGGTGAACGAGAACGACAGCGTGGCAACCGAAGAGATCCGCTTCGGCGACAACGACCGGCTGGCC
GCGCGCGTGGCGCAGGCCGCGGGTGCCGACGCGGTGCTGCTGCTGAGCGATGTCGACGGGCTCTTTACGGCCGACCCGGC
GGCGCCGGACAGCGAACTGGTTGGCCGGATCGAGGGGATCACGCCCGAAGTGCTCGCGATGGCGAGCGCCGCCTCCTCAT
CCGGCTTGGGCTCGGGCGGGATGGTCTCGAAGCTGCAGGCCGCGCAGATCGCCGAGCGGGCCGGTATTGCGCTGGCGATC
ATCAACGGCACCCACGAAGCGCCGATTGCGCGGGCACTGGATACCGGCGTCGGCACCGTGTTCGTTCCGCAGCGCAGCGA
TGGCGCGCGCAAGGCGTGGCTCGGCGGGCGATTGGCTCCGCAAGGCACGCTGCGCGTCGATGCGGGATGTGCTCAAGCGC
TGCAAGGCGGTGCGAGCCTGCTGGCGGCCGGGATCGTCGGCATTTCGGGGGCGTTCCGGCGCGGCGATCTCGTCAGCATC
GTGACGGTGTCGGGCCGGAAGATCGCGCAGGGGCTCGCCGAATACGATTCCTCCGAATGCCAGGCCATCATCGGCAAGCG
CGAGGGTGATCAGGCCGCCATCCTGGGCTATGCCCCGCGCGCAGCAGTGATCCACCGCGATCACATGGTCATGCTTTGA

Upstream 100 bases:

>100_bases
TCGAGCCATTGCTCGATGCCGTGCTGGGATATTTGCCGGACAGCACATCGACCGAGACCAAGGGGTCCGAAGTCGAGGAA
GTCGACGAGGAGGGCGGCGA

Downstream 100 bases:

>100_bases
CAGGGTGATGCAGTGACTATCGCGATAACAGGGGGAACGGGCTTCGTCGGACAGGCGCTGATCGACCTTGCCGAGCGCAA
GGGGGTGAAGCTGCGCTCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MVADLSDLARSQRLVVKVGSSLLVEHGEARADWLKSLAQDLAQLRAKGTKVIVVSSGAIALGAARLGFEKGGRGSLSDAQ
ASASVGQIALAGLWSQALGDVGLTAAQILLSLDDLEDRARYLNASATLGRLLDAGAVPVVNENDSVATEEIRFGDNDRLA
ARVAQAAGADAVLLLSDVDGLFTADPAAPDSELVGRIEGITPEVLAMASAASSSGLGSGGMVSKLQAAQIAERAGIALAI
INGTHEAPIARALDTGVGTVFVPQRSDGARKAWLGGRLAPQGTLRVDAGCAQALQGGASLLAAGIVGISGAFRRGDLVSI
VTVSGRKIAQGLAEYDSSECQAIIGKREGDQAAILGYAPRAAVIHRDHMVML

Sequences:

>Translated_372_residues
MVADLSDLARSQRLVVKVGSSLLVEHGEARADWLKSLAQDLAQLRAKGTKVIVVSSGAIALGAARLGFEKGGRGSLSDAQ
ASASVGQIALAGLWSQALGDVGLTAAQILLSLDDLEDRARYLNASATLGRLLDAGAVPVVNENDSVATEEIRFGDNDRLA
ARVAQAAGADAVLLLSDVDGLFTADPAAPDSELVGRIEGITPEVLAMASAASSSGLGSGGMVSKLQAAQIAERAGIALAI
INGTHEAPIARALDTGVGTVFVPQRSDGARKAWLGGRLAPQGTLRVDAGCAQALQGGASLLAAGIVGISGAFRRGDLVSI
VTVSGRKIAQGLAEYDSSECQAIIGKREGDQAAILGYAPRAAVIHRDHMVML
>Mature_372_residues
MVADLSDLARSQRLVVKVGSSLLVEHGEARADWLKSLAQDLAQLRAKGTKVIVVSSGAIALGAARLGFEKGGRGSLSDAQ
ASASVGQIALAGLWSQALGDVGLTAAQILLSLDDLEDRARYLNASATLGRLLDAGAVPVVNENDSVATEEIRFGDNDRLA
ARVAQAAGADAVLLLSDVDGLFTADPAAPDSELVGRIEGITPEVLAMASAASSSGLGSGGMVSKLQAAQIAERAGIALAI
INGTHEAPIARALDTGVGTVFVPQRSDGARKAWLGGRLAPQGTLRVDAGCAQALQGGASLLAAGIVGISGAFRRGDLVSI
VTVSGRKIAQGLAEYDSSECQAIIGKREGDQAAILGYAPRAAVIHRDHMVML

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=273, Percent_Identity=32.2344322344322, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI21361368, Length=274, Percent_Identity=32.1167883211679, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1786437, Length=369, Percent_Identity=42.8184281842818, Blast_Score=244, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=31.5412186379928, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=31.5412186379928, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=30.2380952380952, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321822, Length=422, Percent_Identity=29.3838862559242, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=30.2405498281787, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 37866; Mature: 37866

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADLSDLARSQRLVVKVGSSLLVEHGEARADWLKSLAQDLAQLRAKGTKVIVVSSGAIA
CCCCHHHHHHHCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEE
LGAARLGFEKGGRGSLSDAQASASVGQIALAGLWSQALGDVGLTAAQILLSLDDLEDRAR
EHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YLNASATLGRLLDAGAVPVVNENDSVATEEIRFGDNDRLAARVAQAAGADAVLLLSDVDG
HHCHHHHHHHHHCCCCCCEECCCCCCCHHHEECCCCHHHHHHHHHHCCCCEEEEEECCCC
LFTADPAAPDSELVGRIEGITPEVLAMASAASSSGLGSGGMVSKLQAAQIAERAGIALAI
CEECCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
INGTHEAPIARALDTGVGTVFVPQRSDGARKAWLGGRLAPQGTLRVDAGCAQALQGGASL
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHCHHHH
LAAGIVGISGAFRRGDLVSIVTVSGRKIAQGLAEYDSSECQAIIGKREGDQAAILGYAPR
HHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCC
AAVIHRDHMVML
EEEEECCCEEEC
>Mature Secondary Structure
MVADLSDLARSQRLVVKVGSSLLVEHGEARADWLKSLAQDLAQLRAKGTKVIVVSSGAIA
CCCCHHHHHHHCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEE
LGAARLGFEKGGRGSLSDAQASASVGQIALAGLWSQALGDVGLTAAQILLSLDDLEDRAR
EHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YLNASATLGRLLDAGAVPVVNENDSVATEEIRFGDNDRLAARVAQAAGADAVLLLSDVDG
HHCHHHHHHHHHCCCCCCEECCCCCCCHHHEECCCCHHHHHHHHHHCCCCEEEEEECCCC
LFTADPAAPDSELVGRIEGITPEVLAMASAASSSGLGSGGMVSKLQAAQIAERAGIALAI
CEECCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
INGTHEAPIARALDTGVGTVFVPQRSDGARKAWLGGRLAPQGTLRVDAGCAQALQGGASL
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHCHHHH
LAAGIVGISGAFRRGDLVSIVTVSGRKIAQGLAEYDSSECQAIIGKREGDQAAILGYAPR
HHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCC
AAVIHRDHMVML
EEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA