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The map label for this gene is ddl

Identifier: 85373189

GI number: 85373189

Start: 406600

End: 407571

Strand: Direct

Name: ddl

Synonym: ELI_01810

Alternate gene names: 85373189

Gene position: 406600-407571 (Clockwise)

Preceding gene: 85373188

Following gene: 85373190

Centisome position: 13.32

GC content: 64.2

Gene sequence:

>972_bases
GTGAGCCTCGACCGTAAACTCCACGTCGTCGTCCTGATGGGCGGCTGGGCGAACGAGCGCGAAGTCTCGCTGATGAGCGG
CGAGGGTGTCGCCAAGGCGCTCGAGAAGCGCGGACACACAGTGACGCGGATCGACATGGACCGGCAGGTTGCTGCGAAGA
TCGCCGAAGCTGCGCCCGATGTCGTCTTCAACGCGCTGCACGGCGTTCCCGGCGAGGACGGCACCGTGCAGGGGATGCTC
GACCTTATGGGTGTGCCCTATACCCATTCGGGGCTCGCCACGTCCGTGATCGCGATCGACAAGGAATTGACCAAGCAGCA
GCTCGTGCCGCGCGGCATCCCCATGCCCGGCGGGCGGATTGTACAGTCGGAAGACCTCTACCAGCAGGACCCGCTGGCAC
GGCCCTATGTGCTGAAGCCGGTCAACGAAGGTTCCTCGGTCGGCGTCGCTATCGTCACCGACGAGAGCAATTACGGCAAT
CCGATCCGTCGCGATGCCCCGGGGCCGTGGCAGGAATTCCGTGAGCTTCTCGCCGAGCCCTTCATCCGCGGGCGCGAACT
GACCACGGCGGTCGTCGGCGGACAGGCGCTGGCCGTGACCGAACTCAAGCCCAAGAGCGGTTTCTACGATTTCGATGCCA
AATATACCGACGGGATGACCGAGCATGTCTGCCCGGCCGATATCCCGCCCGAGATCGAGGCGCTGTGCAAGAAATACGCC
CTCGAGGCGCATCGCATCCTCGGCTGTCGCGGCACCAGTCGCACCGATTATCGCTGGGACGACGAGCAGGGCGACGACGG
ACTGTTCGTGCTTGAAACCAACACGCAGCCCGGCATGACCCCGCTCAGCCTAGTACCCGAGCAGGCCGCCTATGCCGGCA
TGAGTTACGAGGACCTCGTCGAAGCGATCGTGGAGGCTGCGCTCGAACATTTCGCCGCAAAGACCGGAGGGAACGATGGC
GACCAGGGTTAA

Upstream 100 bases:

>100_bases
CCAGCGCCGACATCGAAGGATTGGGCGAAGAAGTGAAGCGCCGGGTCTATGCGCATTCGGGCGTCGAGCTCGAATGGGAA
ATCCAGCGGGTGGGACGCCC

Downstream 100 bases:

>100_bases
GCGCAAGACGCAGGGTGTGCGCCGCTCGACTGCGGCCAGGAACCGGGCGAACAAGGCGCGGCAGGCGCGCGATCGCGGCA
AAGGCCTGTTCGACGGGGCG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSLDRKLHVVVLMGGWANEREVSLMSGEGVAKALEKRGHTVTRIDMDRQVAAKIAEAAPDVVFNALHGVPGEDGTVQGML
DLMGVPYTHSGLATSVIAIDKELTKQQLVPRGIPMPGGRIVQSEDLYQQDPLARPYVLKPVNEGSSVGVAIVTDESNYGN
PIRRDAPGPWQEFRELLAEPFIRGRELTTAVVGGQALAVTELKPKSGFYDFDAKYTDGMTEHVCPADIPPEIEALCKKYA
LEAHRILGCRGTSRTDYRWDDEQGDDGLFVLETNTQPGMTPLSLVPEQAAYAGMSYEDLVEAIVEAALEHFAAKTGGNDG
DQG

Sequences:

>Translated_323_residues
MSLDRKLHVVVLMGGWANEREVSLMSGEGVAKALEKRGHTVTRIDMDRQVAAKIAEAAPDVVFNALHGVPGEDGTVQGML
DLMGVPYTHSGLATSVIAIDKELTKQQLVPRGIPMPGGRIVQSEDLYQQDPLARPYVLKPVNEGSSVGVAIVTDESNYGN
PIRRDAPGPWQEFRELLAEPFIRGRELTTAVVGGQALAVTELKPKSGFYDFDAKYTDGMTEHVCPADIPPEIEALCKKYA
LEAHRILGCRGTSRTDYRWDDEQGDDGLFVLETNTQPGMTPLSLVPEQAAYAGMSYEDLVEAIVEAALEHFAAKTGGNDG
DQG
>Mature_322_residues
SLDRKLHVVVLMGGWANEREVSLMSGEGVAKALEKRGHTVTRIDMDRQVAAKIAEAAPDVVFNALHGVPGEDGTVQGMLD
LMGVPYTHSGLATSVIAIDKELTKQQLVPRGIPMPGGRIVQSEDLYQQDPLARPYVLKPVNEGSSVGVAIVTDESNYGNP
IRRDAPGPWQEFRELLAEPFIRGRELTTAVVGGQALAVTELKPKSGFYDFDAKYTDGMTEHVCPADIPPEIEALCKKYAL
EAHRILGCRGTSRTDYRWDDEQGDDGLFVLETNTQPGMTPLSLVPEQAAYAGMSYEDLVEAIVEAALEHFAAKTGGNDGD
QG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=37.5404530744337, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786579, Length=361, Percent_Identity=29.3628808864266, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ERYLH (Q2NCY7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457251.1
- ProteinModelPortal:   Q2NCY7
- SMR:   Q2NCY7
- STRING:   Q2NCY7
- GeneID:   3868847
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01810
- NMPDR:   fig|314225.3.peg.2727
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- PhylomeDB:   Q2NCY7
- ProtClustDB:   PRK01372
- BioCyc:   ELIT314225:ELI_01810-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35006; Mature: 34875

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDRKLHVVVLMGGWANEREVSLMSGEGVAKALEKRGHTVTRIDMDRQVAAKIAEAAPD
CCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH
VVFNALHGVPGEDGTVQGMLDLMGVPYTHSGLATSVIAIDKELTKQQLVPRGIPMPGGRI
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VQSEDLYQQDPLARPYVLKPVNEGSSVGVAIVTDESNYGNPIRRDAPGPWQEFRELLAEP
EECCCCHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
FIRGRELTTAVVGGQALAVTELKPKSGFYDFDAKYTDGMTEHVCPADIPPEIEALCKKYA
HHCCCHHEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHH
LEAHRILGCRGTSRTDYRWDDEQGDDGLFVLETNTQPGMTPLSLVPEQAAYAGMSYEDLV
HHHHHHEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCHHHHHCCCCHHHHH
EAIVEAALEHFAAKTGGNDGDQG
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLDRKLHVVVLMGGWANEREVSLMSGEGVAKALEKRGHTVTRIDMDRQVAAKIAEAAPD
CCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH
VVFNALHGVPGEDGTVQGMLDLMGVPYTHSGLATSVIAIDKELTKQQLVPRGIPMPGGRI
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
VQSEDLYQQDPLARPYVLKPVNEGSSVGVAIVTDESNYGNPIRRDAPGPWQEFRELLAEP
EECCCCHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
FIRGRELTTAVVGGQALAVTELKPKSGFYDFDAKYTDGMTEHVCPADIPPEIEALCKKYA
HHCCCHHEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHH
LEAHRILGCRGTSRTDYRWDDEQGDDGLFVLETNTQPGMTPLSLVPEQAAYAGMSYEDLV
HHHHHHEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCHHHHHCCCCHHHHH
EAIVEAALEHFAAKTGGNDGDQG
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA