Click here to switch to the map view.

The map label for this gene is fadA [H]

Identifier: 85373122

GI number: 85373122

Start: 339816

End: 340994

Strand: Direct

Name: fadA [H]

Synonym: ELI_01475

Alternate gene names: 85373122

Gene position: 339816-340994 (Clockwise)

Preceding gene: 85373118

Following gene: 85373123

Centisome position: 11.13

GC content: 63.53

Gene sequence:

>1179_bases
ATGCCCATGACCACCGCCTATATCGTCGAAGCCGTTCGTACCGCGGGGGGCCGTCGTGGAGGCCGCCTGGCCGGTGTCCA
CCCGGTCGACCTGCTGGCGAAATCGCTCGATGCGGTGGTCGAGCGCAGCGGAGTGGATCCGGCGGCCATTGACGATGTGG
TGACCGGCTGCGTCAGCCAGGCTGGTGAGCAGGCGATGCAGGTTGGCCGCATGGGCGTGCTGGCCTCCAAGCACCTGCCG
CAATCGACCCCGGCGGTGACGATCGATCGCCAGTGCGGTTCGTCGCAGCAGGCGATCCAGTTCGCGGCGCAAGCGGTGAT
GAGCGGGACGCAGGATGCGGTGATCGCCAGCGGCGTCGAAAGCATGAGCCGGGTGCCGATGGGCTCGAACGCGACCTTCC
ACATGAAGGAAGGGCTGGGCCATTACAAATCGCCGGGCCTCGAAGAGAAATACCCGAAGGTTCAGTTCAGCCAGTTCATG
GGCGCGGAAATGATCGCCAACAAGCATGGCTTCGACAAGGACCAGCTCGACAGCTTCGCGCTCGGCAGTCATGCGAAGGC
GATCAAGGCGACCGAAACCGGCGCTTTCAAAGCCGAGATCGTCCCGGTCGAAGTGGAAACACCCGAAGGCGAGCAGATGC
ACACTGTGGATGAAGGCATCCGCTTCGATGCCACTTTGGACGGCATTGCCGGCGTCAAGCTGATCAGCACGGAAGGAAAG
ATCACCGCCGCCACCTCCAGCCAGATCTGCGACGGGTCGAGCGCTGTGCTGGTGGTGAGCGAGGAATTCCTCAAGACCCA
CAACCTCACCCCGCTGGCGCGCATTCACAACCTGACGGTGACGGCCGGTGATCCGGTCATCATGCTGGAAGAGCCGCTGT
TCGCCACGGACAAGGCGCTGGAGCGCGCGGGCATGAAGATCGACGATATCGATCTTTACGAAGTCAACGAAGCCTTTGCG
CCGGTGCCGCTGGCGTGGCTCAAGCACACTGGCGCGGACCCGGACAAGCTCAACGTCCATGGCGGCGCGATCGCGCTAGG
CCACCCGCTGGGGGCTTCCGGCACCAAGCTGATGGCGACACTCGTCCATGCGCTCAAGCGCCATGACAAGAAGTACGGCC
TGCAGACGATGTGCGAAGGCGGCGGTGTCGCCAATGTGACGATTGTCGAGGCGGTTTAG

Upstream 100 bases:

>100_bases
CGGATGGTGCACCGCGAGCGGAAAACACGGTCCAGAAACCCGGTTGACGCAAACGTAAGGTCACCTTACGCGAGTGGCAA
ATCGCAACGTATCGAGAGGA

Downstream 100 bases:

>100_bases
GGTCTAGCCGGACTTACCCAATCCCGTTCGGGCTGAGCCTGTCGAAGCCCCGTCCTTTTCTTCGGGTGCGCGGTTCCAAA
AAGAAGTACGGCCCTTCGAC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPMTTAYIVEAVRTAGGRRGGRLAGVHPVDLLAKSLDAVVERSGVDPAAIDDVVTGCVSQAGEQAMQVGRMGVLASKHLP
QSTPAVTIDRQCGSSQQAIQFAAQAVMSGTQDAVIASGVESMSRVPMGSNATFHMKEGLGHYKSPGLEEKYPKVQFSQFM
GAEMIANKHGFDKDQLDSFALGSHAKAIKATETGAFKAEIVPVEVETPEGEQMHTVDEGIRFDATLDGIAGVKLISTEGK
ITAATSSQICDGSSAVLVVSEEFLKTHNLTPLARIHNLTVTAGDPVIMLEEPLFATDKALERAGMKIDDIDLYEVNEAFA
PVPLAWLKHTGADPDKLNVHGGAIALGHPLGASGTKLMATLVHALKRHDKKYGLQTMCEGGGVANVTIVEAV

Sequences:

>Translated_392_residues
MPMTTAYIVEAVRTAGGRRGGRLAGVHPVDLLAKSLDAVVERSGVDPAAIDDVVTGCVSQAGEQAMQVGRMGVLASKHLP
QSTPAVTIDRQCGSSQQAIQFAAQAVMSGTQDAVIASGVESMSRVPMGSNATFHMKEGLGHYKSPGLEEKYPKVQFSQFM
GAEMIANKHGFDKDQLDSFALGSHAKAIKATETGAFKAEIVPVEVETPEGEQMHTVDEGIRFDATLDGIAGVKLISTEGK
ITAATSSQICDGSSAVLVVSEEFLKTHNLTPLARIHNLTVTAGDPVIMLEEPLFATDKALERAGMKIDDIDLYEVNEAFA
PVPLAWLKHTGADPDKLNVHGGAIALGHPLGASGTKLMATLVHALKRHDKKYGLQTMCEGGGVANVTIVEAV
>Mature_391_residues
PMTTAYIVEAVRTAGGRRGGRLAGVHPVDLLAKSLDAVVERSGVDPAAIDDVVTGCVSQAGEQAMQVGRMGVLASKHLPQ
STPAVTIDRQCGSSQQAIQFAAQAVMSGTQDAVIASGVESMSRVPMGSNATFHMKEGLGHYKSPGLEEKYPKVQFSQFMG
AEMIANKHGFDKDQLDSFALGSHAKAIKATETGAFKAEIVPVEVETPEGEQMHTVDEGIRFDATLDGIAGVKLISTEGKI
TAATSSQICDGSSAVLVVSEEFLKTHNLTPLARIHNLTVTAGDPVIMLEEPLFATDKALERAGMKIDDIDLYEVNEAFAP
VPLAWLKHTGADPDKLNVHGGAIALGHPLGASGTKLMATLVHALKRHDKKYGLQTMCEGGGVANVTIVEAV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=390, Percent_Identity=37.6923076923077, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI4557237, Length=395, Percent_Identity=33.6708860759494, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI148539872, Length=387, Percent_Identity=34.1085271317829, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=34.0966921119593, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=31.4420803782506, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194353979, Length=385, Percent_Identity=26.4935064935065, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=38.6934673366834, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=39.6039603960396, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=37.6590330788804, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI87082165, Length=386, Percent_Identity=36.7875647668394, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=30.4964539007092, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133906874, Length=405, Percent_Identity=36.0493827160494, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=34.2569269521411, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=32.2335025380711, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=29.4663573085847, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=383, Percent_Identity=30.5483028720627, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=34, Blast_Score=179, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=33.0769230769231, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=37.6590330788804, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24655093, Length=395, Percent_Identity=34.4303797468354, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=31.7258883248731, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=29.6208530805687, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41276; Mature: 41144

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00358 RIBOSOMAL_L5 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTTAYIVEAVRTAGGRRGGRLAGVHPVDLLAKSLDAVVERSGVDPAAIDDVVTGCVSQ
CCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGEQAMQVGRMGVLASKHLPQSTPAVTIDRQCGSSQQAIQFAAQAVMSGTQDAVIASGVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SMSRVPMGSNATFHMKEGLGHYKSPGLEEKYPKVQFSQFMGAEMIANKHGFDKDQLDSFA
HHHCCCCCCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHCCCCCHHHHHHHH
LGSHAKAIKATETGAFKAEIVPVEVETPEGEQMHTVDEGIRFDATLDGIAGVKLISTEGK
CCCCCCEEEECCCCCEEEEEEEEEEECCCCCCEEHHHCCCEEECEECCCCCEEEEECCCC
ITAATSSQICDGSSAVLVVSEEFLKTHNLTPLARIHNLTVTAGDPVIMLEEPLFATDKAL
EEECCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHEEEEEECCCCEEEECCCCHHHHHHH
ERAGMKIDDIDLYEVNEAFAPVPLAWLKHTGADPDKLNVHGGAIALGHPLGASGTKLMAT
HHCCCEECCCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCCHHHHHHH
LVHALKRHDKKYGLQTMCEGGGVANVTIVEAV
HHHHHHHHHHHHCHHHHHCCCCEEEEEEEEEC
>Mature Secondary Structure 
PMTTAYIVEAVRTAGGRRGGRLAGVHPVDLLAKSLDAVVERSGVDPAAIDDVVTGCVSQ
CCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGEQAMQVGRMGVLASKHLPQSTPAVTIDRQCGSSQQAIQFAAQAVMSGTQDAVIASGVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SMSRVPMGSNATFHMKEGLGHYKSPGLEEKYPKVQFSQFMGAEMIANKHGFDKDQLDSFA
HHHCCCCCCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHCCCCCHHHHHHHH
LGSHAKAIKATETGAFKAEIVPVEVETPEGEQMHTVDEGIRFDATLDGIAGVKLISTEGK
CCCCCCEEEECCCCCEEEEEEEEEEECCCCCCEEHHHCCCEEECEECCCCCEEEEECCCC
ITAATSSQICDGSSAVLVVSEEFLKTHNLTPLARIHNLTVTAGDPVIMLEEPLFATDKAL
EEECCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHEEEEEECCCCEEEECCCCHHHHHHH
ERAGMKIDDIDLYEVNEAFAPVPLAWLKHTGADPDKLNVHGGAIALGHPLGASGTKLMAT
HHCCCEECCCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCCHHHHHHH
LVHALKRHDKKYGLQTMCEGGGVANVTIVEAV
HHHHHHHHHHHHCHHHHHCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]