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The map label for this gene is yibK [C]

Identifier: 85373106

GI number: 85373106

Start: 314097

End: 314555

Strand: Direct

Name: yibK [C]

Synonym: ELI_01395

Alternate gene names: 85373106

Gene position: 314097-314555 (Clockwise)

Preceding gene: 85373105

Following gene: 85373107

Centisome position: 10.29

GC content: 64.49

Gene sequence:

>459_bases
GTGACTGCAATCGTCCTCGTCCACCCGGAAATTCCCGGTAACACCGGCGCGGTGGGGCGGACCTGCGTCGCGCTCGACAT
GGAGCTGGTGCTGATCCACCCGCTCGGCTTTGCCATCGACGACAAGCGGGTGAAGCGTTCTGGGCTCGATTACTGGCCCC
ATATCCGGCTCGTCGAATGTGCCAGCTGGGACGCGTTTCTCGCCGAACGCGCGCCGCGCGCGGACCAGCTGTTCCTGTTC
GAGGAATATGCGCCGCGCAGCTTTTACGAGCCGGACTATCCCAAGGATGCCTATCTCGTCTTCGGGCGCGAGACCAAAGG
GTTGCCACAGGGCATAGTCGCGGCCCATCGCGCGCAGATGGTCAGCCTGCCAATGCGTTCGGACAAGGTCCGCTCGCTTA
ACCTCGCCAACACGGTTGCGGCGGCGGCGTATCAGGCGATGCGGGGGCAGCTCAGCTGA

Upstream 100 bases:

>100_bases
GTTCGGCTTCTTCGTCGGCCAGACGATGAAAGCGATGCAGGGCAAAGCCAATCCGGCGGTGGTGAACCAGATCCTCAAGG
ACAAGCTCGGCTGATCAGCC

Downstream 100 bases:

>100_bases
TTGCCAATTCAGGCGGGAGTGGTAACCTGTGCGGCATGAGATGGGCATTCATGATTTTCATCGGGTTGTATGCGCTGGCG
CTCTTCCTGCTGGCGGTCGG

Product: putative RNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MTAIVLVHPEIPGNTGAVGRTCVALDMELVLIHPLGFAIDDKRVKRSGLDYWPHIRLVECASWDAFLAERAPRADQLFLF
EEYAPRSFYEPDYPKDAYLVFGRETKGLPQGIVAAHRAQMVSLPMRSDKVRSLNLANTVAAAAYQAMRGQLS

Sequences:

>Translated_152_residues
MTAIVLVHPEIPGNTGAVGRTCVALDMELVLIHPLGFAIDDKRVKRSGLDYWPHIRLVECASWDAFLAERAPRADQLFLF
EEYAPRSFYEPDYPKDAYLVFGRETKGLPQGIVAAHRAQMVSLPMRSDKVRSLNLANTVAAAAYQAMRGQLS
>Mature_151_residues
TAIVLVHPEIPGNTGAVGRTCVALDMELVLIHPLGFAIDDKRVKRSGLDYWPHIRLVECASWDAFLAERAPRADQLFLFE
EYAPRSFYEPDYPKDAYLVFGRETKGLPQGIVAAHRAQMVSLPMRSDKVRSLNLANTVAAAAYQAMRGQLS

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=40.2684563758389, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16931; Mature: 16800

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVLVHPEIPGNTGAVGRTCVALDMELVLIHPLGFAIDDKRVKRSGLDYWPHIRLVEC
CEEEEEEECCCCCCCCCCCCEEEEECEEEEEEECCCCEECCHHHHHCCCCCCCCEEEEEE
ASWDAFLAERAPRADQLFLFEEYAPRSFYEPDYPKDAYLVFGRETKGLPQGIVAAHRAQM
CCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
VSLPMRSDKVRSLNLANTVAAAAYQAMRGQLS
EECCCCCCCCEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAIVLVHPEIPGNTGAVGRTCVALDMELVLIHPLGFAIDDKRVKRSGLDYWPHIRLVEC
EEEEEEECCCCCCCCCCCCEEEEECEEEEEEECCCCEECCHHHHHCCCCCCCCEEEEEE
ASWDAFLAERAPRADQLFLFEEYAPRSFYEPDYPKDAYLVFGRETKGLPQGIVAAHRAQM
CCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
VSLPMRSDKVRSLNLANTVAAAAYQAMRGQLS
EECCCCCCCCEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]