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The map label for this gene is gatB

Identifier: 85373105

GI number: 85373105

Start: 312594

End: 314090

Strand: Direct

Name: gatB

Synonym: ELI_01390

Alternate gene names: 85373105

Gene position: 312594-314090 (Clockwise)

Preceding gene: 85373104

Following gene: 85373106

Centisome position: 10.24

GC content: 63.26

Gene sequence:

>1497_bases
ATGAGCACTTACCGCATCCAGGGCGCAACCGGCGAATGGGAGGTCGTGATCGGCCTCGAAGTCCACGCGCAGGTCACTTC
CAATGCTAAGCTGTTTTCGGGCGCGTCGACCGAGTTCGGGGCCGAGCCTAACGCGCAGGTCAGCCTCGTCGATGCCGCGA
TGCCGGGCATGCTGCCAGTGCCCAACCGCGAGTGCATCCGCCAGGCGGTGCGCACCGGCATGGCGATCGAGGCGCAGATC
AACACATGGTCCCGGTTCGACCGCAAGAACTACTTCTATGCCGACCTGCCGCAGGGCTACCAGATCAGCCAGCTTTACCA
CCCGCTGGTGGGCGAGGGGCAGCTGCTGATCGAAGCCGACGAGAAGGCGGGCATTTCGGAAGACAAGGTCATCGGAATCG
AGCGCATCCATGTCGAGCAGGATGCCGGCAAGCTGATGCACGACCAGCACCCGACCATGTCCTATGTCGACCTCAACCGC
TGCGGCGTGGCGCTGATGGAAATCGTCAGCCGCCCGGACATGCGCTCGCCCGCCGAGGCGGGGGCCTATGTGCGCAAGCT
GCGCGCGATCCTGCGCTATGTCGGCTCGTGCGACGGCAATATGGAAGAAGGCTCGATGCGCGCAGACGTGAATGTTTCGG
TGCGCCGCCCGGGCGAAGAATTCGGTACGCGGACCGAGACCAAGAACGTCAACTCGGTGCGTTTCGTCATGCAAGTGATC
GAATACGAAGCCAACCGGCAGGTCGACCTGATTGAGAGCGGGGGCGCGGTCGAACAGGAAACCCGCCTGTTCGATCCGGG
CACCGGCACCACGCGGACCATGCGCAGCAAGGAAGACGCGCACGATTATCGCTACTTCCCCGATCCCGACCTGCTGCCGC
TGGAGCTGGAGGACAGTTTCCTCGACGAATGCCGAGCGAGCCTGCCCGAACTGCCCGATGCCAAGCGCCAGCGTTATGAA
AACGAGCTAGGCCTGACGCCCTACAATGCGCGCGAACTGACCGCGGAGGTCGAGACTTTCGCCCGCTTCGAAACGCTGCT
CGCCGCCACCGCCAAGGCCATCGGCGAGGACGAGAAAAAGGTCGCCACGCAAGTCGCCAACTGGGCCCTGTCGGTCGCGC
CGGGCGTGATTAAGTCGCTGGGTGACGAAGCGCATGTCGAAAACGCCACCGCCGAGCGCCAAGCCGCCATACTCAAGATG
CAGGACGCGGGCGAGATTTCGGGCGGCCAGGCCAAGGAAATCTTCGAGATCGTCCTCAAGGAAGGCGGCGACCCCGCCAC
CATCGCCGACGAGAAGGGCCTCAAGCAGGTCAGCGACACCGGCGCCATCGAAGCCGCGATCGACGAGATCCTTGCCAACA
ACGAAGACAAGGTCGAGCAATACAAGTGCGGCAAGGACAAGCTGTTCGGCTTCTTCGTCGGCCAGACGATGAAAGCGATG
CAGGGCAAAGCCAATCCGGCGGTGGTGAACCAGATCCTCAAGGACAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
TGCGCCGAGCACGGCTACGCCGTCACCGAACGGTTCCAGCGCTACGAAACCAGCGTGAACTGTCTCGCCATCCACCAAGA
GAATAGCTAAGGGCCATCCC

Downstream 100 bases:

>100_bases
TCAGCCGTGACTGCAATCGTCCTCGTCCACCCGGAAATTCCCGGTAACACCGGCGCGGTGGGGCGGACCTGCGTCGCGCT
CGACATGGAGCTGGTGCTGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSTYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPNAQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIEAQI
NTWSRFDRKNYFYADLPQGYQISQLYHPLVGEGQLLIEADEKAGISEDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNR
CGVALMEIVSRPDMRSPAEAGAYVRKLRAILRYVGSCDGNMEEGSMRADVNVSVRRPGEEFGTRTETKNVNSVRFVMQVI
EYEANRQVDLIESGGAVEQETRLFDPGTGTTRTMRSKEDAHDYRYFPDPDLLPLELEDSFLDECRASLPELPDAKRQRYE
NELGLTPYNARELTAEVETFARFETLLAATAKAIGEDEKKVATQVANWALSVAPGVIKSLGDEAHVENATAERQAAILKM
QDAGEISGGQAKEIFEIVLKEGGDPATIADEKGLKQVSDTGAIEAAIDEILANNEDKVEQYKCGKDKLFGFFVGQTMKAM
QGKANPAVVNQILKDKLG

Sequences:

>Translated_498_residues
MSTYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPNAQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIEAQI
NTWSRFDRKNYFYADLPQGYQISQLYHPLVGEGQLLIEADEKAGISEDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNR
CGVALMEIVSRPDMRSPAEAGAYVRKLRAILRYVGSCDGNMEEGSMRADVNVSVRRPGEEFGTRTETKNVNSVRFVMQVI
EYEANRQVDLIESGGAVEQETRLFDPGTGTTRTMRSKEDAHDYRYFPDPDLLPLELEDSFLDECRASLPELPDAKRQRYE
NELGLTPYNARELTAEVETFARFETLLAATAKAIGEDEKKVATQVANWALSVAPGVIKSLGDEAHVENATAERQAAILKM
QDAGEISGGQAKEIFEIVLKEGGDPATIADEKGLKQVSDTGAIEAAIDEILANNEDKVEQYKCGKDKLFGFFVGQTMKAM
QGKANPAVVNQILKDKLG
>Mature_497_residues
STYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPNAQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIEAQIN
TWSRFDRKNYFYADLPQGYQISQLYHPLVGEGQLLIEADEKAGISEDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNRC
GVALMEIVSRPDMRSPAEAGAYVRKLRAILRYVGSCDGNMEEGSMRADVNVSVRRPGEEFGTRTETKNVNSVRFVMQVIE
YEANRQVDLIESGGAVEQETRLFDPGTGTTRTMRSKEDAHDYRYFPDPDLLPLELEDSFLDECRASLPELPDAKRQRYEN
ELGLTPYNARELTAEVETFARFETLLAATAKAIGEDEKKVATQVANWALSVAPGVIKSLGDEAHVENATAERQAAILKMQ
DAGEISGGQAKEIFEIVLKEGGDPATIADEKGLKQVSDTGAIEAAIDEILANNEDKVEQYKCGKDKLFGFFVGQTMKAMQ
GKANPAVVNQILKDKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=502, Percent_Identity=38.8446215139442, Blast_Score=352, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI115534094, Length=293, Percent_Identity=40.6143344709898, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=315, Percent_Identity=37.7777777777778, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550808, Length=500, Percent_Identity=34.8, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ERYLH (Q2ND71)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457167.1
- ProteinModelPortal:   Q2ND71
- SMR:   Q2ND71
- STRING:   Q2ND71
- GeneID:   3871857
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01390
- NMPDR:   fig|314225.3.peg.2812
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2ND71
- ProtClustDB:   PRK05477
- BioCyc:   ELIT314225:ELI_01390-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54845; Mature: 54714

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPNAQVSLVDAAMPGMLPV
CCCEEECCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCC
PNRECIRQAVRTGMAIEAQINTWSRFDRKNYFYADLPQGYQISQLYHPLVGEGQLLIEAD
CCHHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEEC
EKAGISEDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNRCGVALMEIVSRPDMRSPAEA
CCCCCCCCCEEEEEEEEEEHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHH
GAYVRKLRAILRYVGSCDGNMEEGSMRADVNVSVRRPGEEFGTRTETKNVNSVRFVMQVI
HHHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCHHHCCCCCCCCCHHHHHHHHHH
EYEANRQVDLIESGGAVEQETRLFDPGTGTTRTMRSKEDAHDYRYFPDPDLLPLELEDSF
HHHCCCCEEEEECCCCCCHHCEEECCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCHHH
LDECRASLPELPDAKRQRYENELGLTPYNARELTAEVETFARFETLLAATAKAIGEDEKK
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VATQVANWALSVAPGVIKSLGDEAHVENATAERQAAILKMQDAGEISGGQAKEIFEIVLK
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHEEECCCCCCCCCHHHHHHHHHHH
EGGDPATIADEKGLKQVSDTGAIEAAIDEILANNEDKVEQYKCGKDKLFGFFVGQTMKAM
CCCCCCEECCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
QGKANPAVVNQILKDKLG
CCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
STYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGASTEFGAEPNAQVSLVDAAMPGMLPV
CCEEECCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCC
PNRECIRQAVRTGMAIEAQINTWSRFDRKNYFYADLPQGYQISQLYHPLVGEGQLLIEAD
CCHHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEEC
EKAGISEDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNRCGVALMEIVSRPDMRSPAEA
CCCCCCCCCEEEEEEEEEEHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHH
GAYVRKLRAILRYVGSCDGNMEEGSMRADVNVSVRRPGEEFGTRTETKNVNSVRFVMQVI
HHHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCHHHCCCCCCCCCHHHHHHHHHH
EYEANRQVDLIESGGAVEQETRLFDPGTGTTRTMRSKEDAHDYRYFPDPDLLPLELEDSF
HHHCCCCEEEEECCCCCCHHCEEECCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCHHH
LDECRASLPELPDAKRQRYENELGLTPYNARELTAEVETFARFETLLAATAKAIGEDEKK
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VATQVANWALSVAPGVIKSLGDEAHVENATAERQAAILKMQDAGEISGGQAKEIFEIVLK
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHEEECCCCCCCCCHHHHHHHHHHH
EGGDPATIADEKGLKQVSDTGAIEAAIDEILANNEDKVEQYKCGKDKLFGFFVGQTMKAM
CCCCCCEECCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
QGKANPAVVNQILKDKLG
CCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA