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The map label for this gene is ptrB [C]

Identifier: 84625430

GI number: 84625430

Start: 4265957

End: 4268050

Strand: Reverse

Name: ptrB [C]

Synonym: XOO_3773

Alternate gene names: 84625430

Gene position: 4268050-4265957 (Counterclockwise)

Preceding gene: 84625431

Following gene: 84625429

Centisome position: 86.39

GC content: 63.09

Gene sequence:

>2094_bases
ATGTCCAAGTTCGCCTCGATCTGCCTGGTTGCGGGCCTGATCACCGCTGGCGCCGTCTCTGCAGAGGAAACCGCCGTGTC
CAACGATCCCTACGCCTGGCTGGAAGATGTCACCGGCGCCAAGCCGTTGGACTGGGTCAAGGCCCAGAACGCCAAAACCG
AAGCGCGGCTGGCCACCACGCCGGCGTTCAAGCAGATGGAAACCAGCATCCGCGAGGTCCTCGATTCGAACGCCAAGATT
CCCGGCGTTCAGAAGATCGGCGCGTTCTACTACAACTTCTGGAAGGATAAGCAGCACGAGCGCGGCCTGTGGCGGCGCAC
CACCCTGGACGAATACCGCAAGGCATCGCCCAAGTGGGAAACGGTGCTGGACCTGGATGCACTCAACAAGGCCGAAGGCG
AAAACTGGGTGTGGCACGGTGCCGATTGCCTGCGTCCGGACTACCAGCGCTGCCTGATTGCGCTGTCGCGCGGCGGTGCC
GACGCCGATGTCACCCGCGAGTTCGATCTGGGAAGCAAGCACTGGGTCAAGGACGGCTTCTTCCGTCCCGAGTCGAAGGG
CGGGCTGGGCTGGATCGACCAGGACACCGTTTACGTGTTCACCGATTTCGGCGCCGGCAGCATGACCAGCTCCGGCTACC
CGCGCATCGCCAAGCAATGGAAGCGCGGCACGCCTCTGACTGCCGCCAGCGTGGTGTACGAAGGCAAGCCGACCGATATG
TACATCGCGGCGATGCATGACGACACGCCCGGATTCGAGCGCGATTTCGTCAGCCGCACGCTGGCCTTCTACAACGACGA
GATGTATCTCAAGAGCGCCGACGGCAAGCTGGTCAAGGTGGACGTGCCCAACTCGGCCAGCAAGTCGGTCAAGCGGCAGT
GGCTGACGCTGGAACTGCGCGAGCCGTGGACGGTGGCTGGCAAAACCTACAAGCCCGGCTCGCTGCTGGCGACGCGTTTT
GACGACTTCATGGCCGGCAAGCGCAACATCGAGGTGTTGTTCGAACCCACCGACACCACCTCGCTGGCCGGCGTGGCGTG
GACCAAGTCGCACCTGGTGTTGAACGTGCTGGAAGACGTCAAGAACCGCCTCAGCGTGATCACGCCCTCGCAGGATGGAT
GGAAAAAGAGCGCGTTTGTCGGTGCACCGAGCTTCGGCACGGTGGATGTCAGCGCGGTGGATAGCGAGGACAGCGATGCG
CTGTGGCTCACCGCCACCGATTATCTGACTCCCACCACGTTGTCGTGGGTGGACGTGGGCTCTGCGCCGCAGCAACTCAA
AACTATGCCGGCGTTCTTCGATGCCGGCAAGGACACCATCGAGCAGCACTTTGCCACCAGCAAGGACGGCACGCGCGTGC
CGTATTTCCTGGTGCGCCCGAAGAGCCTGAAGCTCGACGGCAGTGCGCCGACCTTGCTGTACGGCTACGGCGGGTTCGAG
ATTTCGATGACGCCCAATTACTCCGGCGGTTTGGGTCGTGCGTGGCTGGAAAAGGGCGGCGTCTACGTCGTGGCCAATAT
CCGCGGCGGTGGCGAATACGGCCCGCGCTGGCACCAGGCCGCCCTCAAGCAGAACCGCCACAAGGCATATGAAGACATGG
CCGCGGTGGCCAAGGATCTGGTTGCGCGCAAGATCACCTCGACCAAGCATCTGGGCGTGCAGGGCGGCAGCAATGGCGGT
CTGATGACCGGCAACATGCTCACCCAGTATCCGGAACTGTTCGGTGCGGTGGTGGTGCAGGTGCCACTGCTGGACATGAA
GCGTTACAGCCACCTGCTGGCCGGCGCCTCGTGGATGGCCGAGTACGGCAACCCGGACACCGACGACTGGAATTTCATCC
AGACCTTCTCGCCGTACCAACTGTTCGACCCGAAGAAGACCTACCCACCGGTGATCTTCCTGACCTCCACGCGCGACGAC
CGCGTGCATCCTGGCCACGCCCGCAAGATGGCTGCCAAGATGATCGATGCCGGCAAGGACGTGACCTACTACGAGAACAT
CGAAGGCGGCCACGGCGGTGCGGCCAATAACGCGCAGGCTGCGCATATGGCAGCGCTGGCCTACAGTTTCCTGTGGGAGC
GGCTGGCCGACTGA

Upstream 100 bases:

>100_bases
TCGCCTACGGCGATACAGGCGCGTGCAGCCTGACCTTCGGCAGGGTTGGCCGGCGGGGCGTTGTGCTACGTTCCGGGCAT
TTCTTTGTCCGGAAACATTC

Downstream 100 bases:

>100_bases
GTGAGAGATCGGCGCGCCGGGCAAGGATATCCGGCGCGATGACTGCACTTGCCGAAGTTGCTTTCCGGCGCAAGTGATCG
CCATGTCCCCGCCGGCCCAA

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSKFASICLVAGLITAGAVSAEETAVSNDPYAWLEDVTGAKPLDWVKAQNAKTEARLATTPAFKQMETSIREVLDSNAKI
PGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKASPKWETVLDLDALNKAEGENWVWHGADCLRPDYQRCLIALSRGGA
DADVTREFDLGSKHWVKDGFFRPESKGGLGWIDQDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAASVVYEGKPTDM
YIAAMHDDTPGFERDFVSRTLAFYNDEMYLKSADGKLVKVDVPNSASKSVKRQWLTLELREPWTVAGKTYKPGSLLATRF
DDFMAGKRNIEVLFEPTDTTSLAGVAWTKSHLVLNVLEDVKNRLSVITPSQDGWKKSAFVGAPSFGTVDVSAVDSEDSDA
LWLTATDYLTPTTLSWVDVGSAPQQLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKSLKLDGSAPTLLYGYGGFE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGG
LMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWNFIQTFSPYQLFDPKKTYPPVIFLTSTRDD
RVHPGHARKMAAKMIDAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD

Sequences:

>Translated_697_residues
MSKFASICLVAGLITAGAVSAEETAVSNDPYAWLEDVTGAKPLDWVKAQNAKTEARLATTPAFKQMETSIREVLDSNAKI
PGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKASPKWETVLDLDALNKAEGENWVWHGADCLRPDYQRCLIALSRGGA
DADVTREFDLGSKHWVKDGFFRPESKGGLGWIDQDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAASVVYEGKPTDM
YIAAMHDDTPGFERDFVSRTLAFYNDEMYLKSADGKLVKVDVPNSASKSVKRQWLTLELREPWTVAGKTYKPGSLLATRF
DDFMAGKRNIEVLFEPTDTTSLAGVAWTKSHLVLNVLEDVKNRLSVITPSQDGWKKSAFVGAPSFGTVDVSAVDSEDSDA
LWLTATDYLTPTTLSWVDVGSAPQQLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKSLKLDGSAPTLLYGYGGFE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGG
LMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWNFIQTFSPYQLFDPKKTYPPVIFLTSTRDD
RVHPGHARKMAAKMIDAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
>Mature_696_residues
SKFASICLVAGLITAGAVSAEETAVSNDPYAWLEDVTGAKPLDWVKAQNAKTEARLATTPAFKQMETSIREVLDSNAKIP
GVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKASPKWETVLDLDALNKAEGENWVWHGADCLRPDYQRCLIALSRGGAD
ADVTREFDLGSKHWVKDGFFRPESKGGLGWIDQDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAASVVYEGKPTDMY
IAAMHDDTPGFERDFVSRTLAFYNDEMYLKSADGKLVKVDVPNSASKSVKRQWLTLELREPWTVAGKTYKPGSLLATRFD
DFMAGKRNIEVLFEPTDTTSLAGVAWTKSHLVLNVLEDVKNRLSVITPSQDGWKKSAFVGAPSFGTVDVSAVDSEDSDAL
WLTATDYLTPTTLSWVDVGSAPQQLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKSLKLDGSAPTLLYGYGGFEI
SMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGGL
MTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWNFIQTFSPYQLFDPKKTYPPVIFLTSTRDDR
VHPGHARKMAAKMIDAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=378, Percent_Identity=31.7460317460317, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI284172438, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI284172431, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI284172420, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI284172413, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI70778815, Length=203, Percent_Identity=27.0935960591133, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI108860686, Length=203, Percent_Identity=27.0935960591133, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI108860692, Length=200, Percent_Identity=26.5, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788150, Length=695, Percent_Identity=23.7410071942446, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24583414, Length=363, Percent_Identity=33.0578512396694, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI221510989, Length=265, Percent_Identity=34.3396226415094, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 77078; Mature: 76946

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFASICLVAGLITAGAVSAEETAVSNDPYAWLEDVTGAKPLDWVKAQNAKTEARLATT
CCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCEEECC
PAFKQMETSIREVLDSNAKIPGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKASPKWE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHHCCCCCHH
TVLDLDALNKAEGENWVWHGADCLRPDYQRCLIALSRGGADADVTREFDLGSKHWVKDGF
EEEEHHHHCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHCCC
FRPESKGGLGWIDQDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAASVVYEGKPTDM
CCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCE
YIAAMHDDTPGFERDFVSRTLAFYNDEMYLKSADGKLVKVDVPNSASKSVKRQWLTLELR
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHEEEEEEEC
EPWTVAGKTYKPGSLLATRFDDFMAGKRNIEVLFEPTDTTSLAGVAWTKSHLVLNVLEDV
CCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHH
KNRLSVITPSQDGWKKSAFVGAPSFGTVDVSAVDSEDSDALWLTATDYLTPTTLSWVDVG
HHHHEEECCCCCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEEEECC
SAPQQLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKSLKLDGSAPTLLYGYGGFE
CCHHHHHHCHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCEEEEECCCEE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDL
EEECCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VARKITSTKHLGVQGGSNGGLMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMA
HHHHHCCCCEECCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYGNPDTDDWNFIQTFSPYQLFDPKKTYPPVIFLTSTRDDRVHPGHARKMAAKMIDAGKD
HCCCCCCCCCCEEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKFASICLVAGLITAGAVSAEETAVSNDPYAWLEDVTGAKPLDWVKAQNAKTEARLATT
CHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCCCCEEECC
PAFKQMETSIREVLDSNAKIPGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKASPKWE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHHCCCCCHH
TVLDLDALNKAEGENWVWHGADCLRPDYQRCLIALSRGGADADVTREFDLGSKHWVKDGF
EEEEHHHHCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHCCC
FRPESKGGLGWIDQDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAASVVYEGKPTDM
CCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCE
YIAAMHDDTPGFERDFVSRTLAFYNDEMYLKSADGKLVKVDVPNSASKSVKRQWLTLELR
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHEEEEEEEC
EPWTVAGKTYKPGSLLATRFDDFMAGKRNIEVLFEPTDTTSLAGVAWTKSHLVLNVLEDV
CCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHH
KNRLSVITPSQDGWKKSAFVGAPSFGTVDVSAVDSEDSDALWLTATDYLTPTTLSWVDVG
HHHHEEECCCCCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEEEECC
SAPQQLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKSLKLDGSAPTLLYGYGGFE
CCHHHHHHCHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCEEEEECCCEE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDL
EEECCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VARKITSTKHLGVQGGSNGGLMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMA
HHHHHCCCCEECCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYGNPDTDDWNFIQTFSPYQLFDPKKTYPPVIFLTSTRDDRVHPGHARKMAAKMIDAGKD
HCCCCCCCCCCEEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]