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The map label for this gene is hsdBR [H]

Identifier: 84625385

GI number: 84625385

Start: 4214732

End: 4215826

Strand: Reverse

Name: hsdBR [H]

Synonym: XOO_3728

Alternate gene names: 84625385

Gene position: 4215826-4214732 (Counterclockwise)

Preceding gene: 84625386

Following gene: 84625384

Centisome position: 85.34

GC content: 57.44

Gene sequence:

>1095_bases
GTGAGCTTGCCTCCCTACGTCGACCGGCTAACCATTCACCAGCGTCTGCCCCTCATCTTTCAGGACGGCACTCCCAATCG
CAACTATTGCATTCGGGAAATGGCCGCCTCGGCGGTGTTTGTGATGCTATACATCGGGGCAGTCGAAGGCACTGACCGAT
GGCTTGCGCCGAAACATGTGATGCGTATGACGGTCGAGCAAGCACTGCTGAGTGACGATGCAGTGCGAGAAGCCTACGGC
TTCGGAGCCATGAAGCCGGGGTTTCGAGTAGCTGGGCAACGTTGGTACGAGGAAAACAGTCGCGAGCCCCTTCGCGACGA
GACTTTGCGCCAAGGATTTATCACGAATAACGCTGTGGTAGAGCGGGCAGGGTTGCCAACAACCTCTGGCCTGCCGCGCT
ATGCGCTCAAGACTGACTTCGCAGCGTTATTTGACCCTGCACTTGCTGGAGACGACCTGGCCGCATCCATCACTGCTTGG
CAGGAAGCTCACCTTTCAGCATCGGCGATGGCGCGCGTCGCACTTGTGCGGCGTGGTGCTGTGACAACAGGTGATGATGT
AATAGTGACCTTTCCAAACCGCGAAACGCGCCGGATGGCACCCGGCCCGAGCTCAGTCATCTCGAAAGGGGTCATTGAAG
AATTTGCCGCTCGCTTCCTCACGCAGCCAGCCGTTTTATGGGTGAGCGAGAGCGGCGCGAAAGTCGTCGCTAGAGACGAT
GAACTTGCCAAGTCTCTGAACTTAAAAATTACAGCCGACCGCAACTTGCCAGACATCATCCTTGTAGACCTAGGCGGTGG
TGGCGCACCGGGCTTCCTTCTGGTGTTCATCGAAGTTGTCGCGACCGATGGGCCAATCACGATGCAACGGCAAGAAGCCT
TCATGCAGATTGCAGCCGATGCCGGTTTCAAGCCGGACACGGTAGCCTTCGTGACGGCCTATCTAGACCGCTCCAACGCG
GCTTTCAAAAAGACAATAGCCGAGCTCGCTTGGCACTCCTTCGCTTGGTTTGCTTCCGAGCCCGAGCACATCATCGCCTT
GCACAACGGAGCGACCTCGCCGCTTCCGTTGAGCACGTTGATGCACGGCGTTTAA

Upstream 100 bases:

>100_bases
TCCCCACAGCAAGCGACCTGAAGAAATTGGTTGGCAGCAAAGTCACGCGCGCGAGTGTCGAGAAGGCCTGCGCCAATCTG
TATGCGACAGAAGGTGAAGC

Downstream 100 bases:

>100_bases
CAACCTATTTATTTCTGCCCACCCTCGTCCATCACGCGAGTAGCGATGTACTGAATCCAATAGATCGTTGTGCAATAAAA
ATTACCGTTGGAATTGACAG

Product: restriction endonuclease homolog R.XphI

Products: NA

Alternate protein names: R.BsuBI; Endonuclease BsuBI; Type II restriction enzyme BsuBI [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSLPPYVDRLTIHQRLPLIFQDGTPNRNYCIREMAASAVFVMLYIGAVEGTDRWLAPKHVMRMTVEQALLSDDAVREAYG
FGAMKPGFRVAGQRWYEENSREPLRDETLRQGFITNNAVVERAGLPTTSGLPRYALKTDFAALFDPALAGDDLAASITAW
QEAHLSASAMARVALVRRGAVTTGDDVIVTFPNRETRRMAPGPSSVISKGVIEEFAARFLTQPAVLWVSESGAKVVARDD
ELAKSLNLKITADRNLPDIILVDLGGGGAPGFLLVFIEVVATDGPITMQRQEAFMQIAADAGFKPDTVAFVTAYLDRSNA
AFKKTIAELAWHSFAWFASEPEHIIALHNGATSPLPLSTLMHGV

Sequences:

>Translated_364_residues
MSLPPYVDRLTIHQRLPLIFQDGTPNRNYCIREMAASAVFVMLYIGAVEGTDRWLAPKHVMRMTVEQALLSDDAVREAYG
FGAMKPGFRVAGQRWYEENSREPLRDETLRQGFITNNAVVERAGLPTTSGLPRYALKTDFAALFDPALAGDDLAASITAW
QEAHLSASAMARVALVRRGAVTTGDDVIVTFPNRETRRMAPGPSSVISKGVIEEFAARFLTQPAVLWVSESGAKVVARDD
ELAKSLNLKITADRNLPDIILVDLGGGGAPGFLLVFIEVVATDGPITMQRQEAFMQIAADAGFKPDTVAFVTAYLDRSNA
AFKKTIAELAWHSFAWFASEPEHIIALHNGATSPLPLSTLMHGV
>Mature_363_residues
SLPPYVDRLTIHQRLPLIFQDGTPNRNYCIREMAASAVFVMLYIGAVEGTDRWLAPKHVMRMTVEQALLSDDAVREAYGF
GAMKPGFRVAGQRWYEENSREPLRDETLRQGFITNNAVVERAGLPTTSGLPRYALKTDFAALFDPALAGDDLAASITAWQ
EAHLSASAMARVALVRRGAVTTGDDVIVTFPNRETRRMAPGPSSVISKGVIEEFAARFLTQPAVLWVSESGAKVVARDDE
LAKSLNLKITADRNLPDIILVDLGGGGAPGFLLVFIEVVATDGPITMQRQEAFMQIAADAGFKPDTVAFVTAYLDRSNAA
FKKTIAELAWHSFAWFASEPEHIIALHNGATSPLPLSTLMHGV

Specific function: Recognizes the double-stranded sequence CTGCAG and cleaves after A-5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009528 [H]

Pfam domain/function: PF06616 BsuBI_PstI_RE [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 39701; Mature: 39570

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPPYVDRLTIHQRLPLIFQDGTPNRNYCIREMAASAVFVMLYIGAVEGTDRWLAPKHV
CCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
MRMTVEQALLSDDAVREAYGFGAMKPGFRVAGQRWYEENSREPLRDETLRQGFITNNAVV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEE
ERAGLPTTSGLPRYALKTDFAALFDPALAGDDLAASITAWQEAHLSASAMARVALVRRGA
EECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VTTGDDVIVTFPNRETRRMAPGPSSVISKGVIEEFAARFLTQPAVLWVSESGAKVVARDD
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCH
ELAKSLNLKITADRNLPDIILVDLGGGGAPGFLLVFIEVVATDGPITMQRQEAFMQIAAD
HHHHHCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
AGFKPDTVAFVTAYLDRSNAAFKKTIAELAWHSFAWFASEPEHIIALHNGATSPLPLSTL
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHH
MHGV
HHCC
>Mature Secondary Structure 
SLPPYVDRLTIHQRLPLIFQDGTPNRNYCIREMAASAVFVMLYIGAVEGTDRWLAPKHV
CCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
MRMTVEQALLSDDAVREAYGFGAMKPGFRVAGQRWYEENSREPLRDETLRQGFITNNAVV
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEE
ERAGLPTTSGLPRYALKTDFAALFDPALAGDDLAASITAWQEAHLSASAMARVALVRRGA
EECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VTTGDDVIVTFPNRETRRMAPGPSSVISKGVIEEFAARFLTQPAVLWVSESGAKVVARDD
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCH
ELAKSLNLKITADRNLPDIILVDLGGGGAPGFLLVFIEVVATDGPITMQRQEAFMQIAAD
HHHHHCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
AGFKPDTVAFVTAYLDRSNAAFKKTIAELAWHSFAWFASEPEHIIALHNGATSPLPLSTL
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHH
MHGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1480472 [H]