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The map label for this gene is zraS [H]

Identifier: 84625369

GI number: 84625369

Start: 4196210

End: 4197712

Strand: Reverse

Name: zraS [H]

Synonym: XOO_3712

Alternate gene names: 84625369

Gene position: 4197712-4196210 (Counterclockwise)

Preceding gene: 84625370

Following gene: 84625368

Centisome position: 84.97

GC content: 63.94

Gene sequence:

>1503_bases
ATGCCGCATGAGGGGGGCAATCTGGAGCAGTTGAAGATCCTTCTGGTGGAAGATTCACCAGAAGATGCCGAGCTGTTGTC
CGATCAGTTGCTGGATGCCGGACTGGATGCGGCATTCGAGCGGGTGGACAGCGAGCCGTCGCTGCGCGCCGCGCTGGATG
CGTTTCAGCCGGACATCGTGCTGTCGGATCTGAGCATGCCGGGTTTCTCTGGGCACCAGGCGCTGCGGCTGGTGCGCCAG
AATGGTGCCACGCCTTTCATCTTCGTCTCCGGCACCATGGGTGAAGAGACGGCAGTCAAGGCACTGCAGGACGGCGCCAA
TGACTACATCATCAAACACAATCCGACGCGTTTGCCGAGCGCGGTGATCCGTGCGATCCGCGAGGCGCGCGCCGATCTGG
AACGCCAGCGCGTGGAAAGCGAGCTGATGCGCGCGCAGCGTCTGGAAAGCCTGGCAATGCTGGCGGCCGGTCTGAGCCAC
GACCTGCGCAATATCCTGCAGCCGTTGTTGATCGTGCCCGATCTGCTGGCCGGGCGCACTGACGACCCGCAGCTGCGTCA
GCTGGCCAATGTGGTGGCCGAATGCGGCCGGCGCGGGCATGAAATGGCCGAATCGATGTTGTCGTTCGTGCGTGGGTCCA
ACAAGCCGCGCGAGCAGGTGTCCATCACCAATCTATTCCAGGCCGTGCAGATGCTGCTCAAGAGCAGCCTGCCCGATGGC
GTGCGCCTGCAGATGGACGCCATCGCCCCGGATCTCACCATCGAGGCCAACTACACCGAATTGCAGCAATGCCTGCTCAA
CCTCGGCTTGAACGCGATCCAGGCGATGCCGGAGGGCGGCAGGCTGATCCTGGCCGCCGATCGCTATGATGCCTCGCGCG
TGCGCATGAGCGTGGCCGACACCGGCGTGGGCATGAGCGATGAAACCCGCGCGCGCCTGTTCAGCCCGTTCTTCACCACC
AAGGCGGATGGCACCGGGTTGGGACTGATTTCGTGCAAGCGCATCATCGAAAGCTATGGCGGCAGCATCGTGGTCGATAG
CCGGCTGGACGAAGGCACGCGCTTTGACATGATCGTGCCGATGCGCGCGGTGTCGGCGGTGGTGACGGAAGCCGAACAGC
CGCTCCCGCTTGGCCATGGGCAGCGCATTTTGCTGGTGGATGGCGAGGCCACGCGCCTGTCGTTGCTGGGGAATGCACTT
TCCAGCCAGGGCTATCAGCCGCAGCTGGCCGGCGATGGTGCCGCGGCGCTGCAGTTGGTGCAGCAGCACGCCATGCCGGA
CCTGGTGATCATCGACAGCGACATCATCCTGCTCTCGGCAGTGAGCGTGCTGTTGAGCATGCAGGAGCTGGGGTACCAGG
GCCCGGCGATCGTGCTGGAAGATGTTGGCGCGCCGCTGCAGCGTTCGCATTTTCCGTCGGATCTGCCGGTGCATGTATTG
CGCAAACCCCTGGAAATGCGCCGGGTCTTTCGCGCCGTTTCGCACGCGCTGGAAGTGGCCTGA

Upstream 100 bases:

>100_bases
CGTGGTCAAGCCGGTGGATGTGGATCAGTTCTTCAACGCGGTCAAGACGCTCGGCACGTTCTGGGCGGTCATCAACCAGG
CACCGGAGCTGGACTGAACC

Downstream 100 bases:

>100_bases
CAGCACGTTTCTGCGCACCCGATGCGGGCTGGATAGATGCGATACGATTGCAACAGTTGACGCCCACGCCGGCCGCATGC
GCGAATAACGTATGACTACC

Product: histidine kinase-response regulator hybrid protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLDAGLDAAFERVDSEPSLRAALDAFQPDIVLSDLSMPGFSGHQALRLVRQ
NGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSH
DLRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSITNLFQAVQMLLKSSLPDG
VRLQMDAIAPDLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTT
KADGTGLGLISCKRIIESYGGSIVVDSRLDEGTRFDMIVPMRAVSAVVTEAEQPLPLGHGQRILLVDGEATRLSLLGNAL
SSQGYQPQLAGDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRSHFPSDLPVHVL
RKPLEMRRVFRAVSHALEVA

Sequences:

>Translated_500_residues
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLDAGLDAAFERVDSEPSLRAALDAFQPDIVLSDLSMPGFSGHQALRLVRQ
NGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSH
DLRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSITNLFQAVQMLLKSSLPDG
VRLQMDAIAPDLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTT
KADGTGLGLISCKRIIESYGGSIVVDSRLDEGTRFDMIVPMRAVSAVVTEAEQPLPLGHGQRILLVDGEATRLSLLGNAL
SSQGYQPQLAGDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRSHFPSDLPVHVL
RKPLEMRRVFRAVSHALEVA
>Mature_499_residues
PHEGGNLEQLKILLVEDSPEDAELLSDQLLDAGLDAAFERVDSEPSLRAALDAFQPDIVLSDLSMPGFSGHQALRLVRQN
GATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHD
LRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSITNLFQAVQMLLKSSLPDGV
RLQMDAIAPDLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTTK
ADGTGLGLISCKRIIESYGGSIVVDSRLDEGTRFDMIVPMRAVSAVVTEAEQPLPLGHGQRILLVDGEATRLSLLGNALS
SQGYQPQLAGDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRSHFPSDLPVHVLR
KPLEMRRVFRAVSHALEVA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=31.8548387096774, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=32.034632034632, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790300, Length=269, Percent_Identity=29.7397769516729, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788713, Length=220, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693157, Length=378, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081816, Length=343, Percent_Identity=25.6559766763848, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=186, Percent_Identity=27.9569892473118, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789149, Length=281, Percent_Identity=24.5551601423488, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54287; Mature: 54156

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLDAGLDAAFERVDSEPSLRAALDAFQPDIV
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEE
LSDLSMPGFSGHQALRLVRQNGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPS
EECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCHH
AVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHDLRNILQPLLIVPDLLAGRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSITNLFQAVQMLLKSSLPDG
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRLQMDAIAPDLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVAD
CEEEECCCCCCCEEECCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCHHHHEEEHHH
TGVGMSDETRARLFSPFFTTKADGTGLGLISCKRIIESYGGSIVVDSRLDEGTRFDMIVP
CCCCCCCCHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEHH
MRAVSAVVTEAEQPLPLGHGQRILLVDGEATRLSLLGNALSSQGYQPQLAGDGAAALQLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRSHFPSDLPVHVL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCCCHHHH
RKPLEMRRVFRAVSHALEVA
HCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHEGGNLEQLKILLVEDSPEDAELLSDQLLDAGLDAAFERVDSEPSLRAALDAFQPDIV
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEE
LSDLSMPGFSGHQALRLVRQNGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPS
EECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCHH
AVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHDLRNILQPLLIVPDLLAGRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSITNLFQAVQMLLKSSLPDG
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRLQMDAIAPDLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVAD
CEEEECCCCCCCEEECCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCHHHHEEEHHH
TGVGMSDETRARLFSPFFTTKADGTGLGLISCKRIIESYGGSIVVDSRLDEGTRFDMIVP
CCCCCCCCHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEHH
MRAVSAVVTEAEQPLPLGHGQRILLVDGEATRLSLLGNALSSQGYQPQLAGDGAAALQLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRSHFPSDLPVHVL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCCCHHHH
RKPLEMRRVFRAVSHALEVA
HCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]