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The map label for this gene is purR [H]

Identifier: 84625354

GI number: 84625354

Start: 4176176

End: 4177252

Strand: Reverse

Name: purR [H]

Synonym: XOO_3697

Alternate gene names: 84625354

Gene position: 4177252-4176176 (Counterclockwise)

Preceding gene: 84625355

Following gene: 84625353

Centisome position: 84.56

GC content: 64.72

Gene sequence:

>1077_bases
ATGCGCAGGCCTACCATCAAAGATGTCGCCGAACGCGCCAAGGTCTCGTTGAAGACCGTGTCGCGGGTGATCAACAACGA
GCCGTCAGTGATGCAGGCCACGCGTGCGCGGGTGCTACGCGCCATTGCCGATCTCGACTACGAACCCGACCCGTCCGCGC
GCAATCTGCGCAGCGGCACGCCGTTCGTGATCGGGGTGGTCTACGACAACCCCAACCCGTACCACATCATCGGCATCCAG
AACGGCGTGCTGGCTGCGTGCCGTGAAACCGGTTTTGGCTTGCAGATCCACCCCTGCGATTCGACCTCGCCGTTGCTGGC
CGAAGAACTGGCCGAATGGGTGCAGCGCTCGCGTCTGGCCGGTGTGGTGCTGACCGCACCGATCTCCGAACGCCCGGAGT
TGCTGGCCGGTCTGGCGGCGCGCGGTATCAAGAGCGTACGCATCATCGCCGCCACCGATGACCCGGGCAATGGCCCGTGC
GTGTATATCGACGACCGCGATGCCGCGTATGAAATCACCGAGCATCTGATCCAGCTCGGCCATCAACGCATCGGCTTCTT
GTGGGGTGGTCCGCAGCATCGTTCCAGCGGCGAGCGCTATGCCGGCTACGAAGCGGCGTTGAAGGACTATGGCATTGCGC
TGGACAAGCACCTGGTCATTCCCGGCGATTACACCTTCGACGATGGCTTCCGTGGTGCACGTCGCTTGCTGTCGCTGCGC
GAGCCACCCACCGCCATCTTCGGCAGCAACGACGAAATCGCCGCCGGCGTGTTGGCCGCAGCCAGATCCACCAGCATGAA
CGTCCCGTACGACTTGTCGATTGCCGGGTTCGAAGACAGCCCGTTTTCGCGCCAGTCGTGGCCGGCATTGACCACAGCCA
AGCAGGCCACCGACGACATCGCGCGGCATGCCGCACGCCTGTTGATCAGCCAGCTGCGCAGCGATGCCTACGACGACCAG
CCCGCGCAACTGCAGAACCGTGGCTTCGTGCCGCAACTGGTGGTGCGCGGCTCCACCGCGCCGGCGCCTGCGTCCACCGG
CAAACCCCTTCCCCCGAATCCGCCTGAGCCACGATGA

Upstream 100 bases:

>100_bases
TACATCCTGTTCTATTCGCTGCGCGGTCACCGCGTGGGCCTGCCGGCCCAGGCCAAGTGATCGCGGCGGCATGATGCGTT
CCGACGGACAAGGTACATCC

Downstream 100 bases:

>100_bases
GCCTTCCCACCGAAACCGATACCCTGATGTTCCGCGAAGCGGCGCAGACCGCCGATGTGGTTGCTGCACAGTTCGCACGT
AACGCCGACACCATCGCTGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGVVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPISERPELLAGLAARGIKSVRIIAATDDPGNGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGIALDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAARSTSMNVPYDLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQNRGFVPQLVVRGSTAPAPASTGKPLPPNPPEPR

Sequences:

>Translated_358_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGVVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPISERPELLAGLAARGIKSVRIIAATDDPGNGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGIALDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAARSTSMNVPYDLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQNRGFVPQLVVRGSTAPAPASTGKPLPPNPPEPR
>Mature_358_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGVVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPISERPELLAGLAARGIKSVRIIAATDDPGNGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGIALDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAARSTSMNVPYDLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQNRGFVPQLVVRGSTAPAPASTGKPLPPNPPEPR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=31.6715542521994, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=30.7017543859649, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790194, Length=344, Percent_Identity=30.8139534883721, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.4470989761092, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=29.7945205479452, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788474, Length=343, Percent_Identity=30.0291545189504, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.8481012658228, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786540, Length=300, Percent_Identity=26.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=28, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=24.2990654205607, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=26.031746031746, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.030303030303, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787906, Length=297, Percent_Identity=27.6094276094276, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38857; Mature: 38857

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVIGVVYDNPNPYHIIGIQNGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLA
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GVVLTAPISERPELLAGLAARGIKSVRIIAATDDPGNGPCVYIDDRDAAYEITEHLIQLG
CEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHC
HQRIGFLWGGPQHRSSGERYAGYEAALKDYGIALDKHLVIPGDYTFDDGFRGARRLLSLR
HHHEEEEECCCCCCCCCCCCCCHHHHHHHHCEEECCEEECCCCCCCCCCHHHHHHHHHCC
EPPTAIFGSNDEIAAGVLAAARSTSMNVPYDLSIAGFEDSPFSRQSWPALTTAKQATDDI
CCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ARHAARLLISQLRSDAYDDQPAQLQNRGFVPQLVVRGSTAPAPASTGKPLPPNPPEPR
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVIGVVYDNPNPYHIIGIQNGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLA
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GVVLTAPISERPELLAGLAARGIKSVRIIAATDDPGNGPCVYIDDRDAAYEITEHLIQLG
CEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHC
HQRIGFLWGGPQHRSSGERYAGYEAALKDYGIALDKHLVIPGDYTFDDGFRGARRLLSLR
HHHEEEEECCCCCCCCCCCCCCHHHHHHHHCEEECCEEECCCCCCCCCCHHHHHHHHHCC
EPPTAIFGSNDEIAAGVLAAARSTSMNVPYDLSIAGFEDSPFSRQSWPALTTAKQATDDI
CCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ARHAARLLISQLRSDAYDDQPAQLQNRGFVPQLVVRGSTAPAPASTGKPLPPNPPEPR
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA