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The map label for this gene is gfa [H]

Identifier: 84625303

GI number: 84625303

Start: 4123372

End: 4123995

Strand: Reverse

Name: gfa [H]

Synonym: XOO_3646

Alternate gene names: 84625303

Gene position: 4123995-4123372 (Counterclockwise)

Preceding gene: 84625304

Following gene: 84625302

Centisome position: 83.48

GC content: 62.02

Gene sequence:

>624_bases
ATGACGCATGTGTCGATTCATTCTTCGGTAGATGGCGGTGTTGCGCGAAGCGGCGCGGAAGGTTTTCAAGGCGGCACCCT
TAAATGCCACTGCGCCAGTGACAAGGTGATCGTCGACGTGTCTGCGCAGACCGCGCCCAACCATGCCTGTGGTTGCAGCA
AGTGCTGGAAGCCCGACGGCGCGAAGTTCTCCGTGGTGGCGGTGGTCTCGCGCGACACGGTCAAGGTGACGGCGCACGAA
GAGAAGCTGATGCTCGTCGATGAAAGCGCGACGATCCAATGCCACGCGTGCACCGGGTGCGGCGTGCATCTGTATGGCCG
GATCGAGAACAACGACCACGCGTTCTACGGCCTGGATTTCGTGTCCACCGAGTTGTCGAAGCAACAGGGCTGGTCGGCGC
CCGGTTTTGCTGCGTTCGTGTCGTCCATGATCGAAACCGGCACGCCGCCGGAGCAGATGGATGGGGTGCGCGTGCCTGAC
CGAACTCGGGTTGCCGCCATACGACTGCCTGTCGCCGGCGTTGATGGATGCCCTGTCTGCGCATGTCGCGCGCAACAAGG
GTCTGTTGCATTGATGGTCCAACCCTGCAGCCGGCGATCCGGCTGCGGGGTTTTCTCTTCGTAA

Upstream 100 bases:

>100_bases
GCAAGTCGATCCGGACCGTGATTCATTTCTGACCGTACGGCCAGGAGCGGCGCGTGGCCGCTTCCGCGCACGTAGTACAC
AAGACTCCGGGAGGGGCGTC

Downstream 100 bases:

>100_bases
GGAATCTTCATGGAACGTATCGAACATCGTGCCTGTTTTGGCGGTTGGCAGGATGTCTACCGGCATCAGTCGCAGACCTT
GGACTGCCGCATGCACGTGG

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTHVSIHSSVDGGVARSGAEGFQGGTLKCHCASDKVIVDVSAQTAPNHACGCSKCWKPDGAKFSVVAVVSRDTVKVTAHE
EKLMLVDESATIQCHACTGCGVHLYGRIENNDHAFYGLDFVSTELSKQQGWSAPGFAAFVSSMIETGTPPEQMDGVRVPD
RTRVAAIRLPVAGVDGCPVCACRAQQGSVALMVQPCSRRSGCGVFSS

Sequences:

>Translated_207_residues
MTHVSIHSSVDGGVARSGAEGFQGGTLKCHCASDKVIVDVSAQTAPNHACGCSKCWKPDGAKFSVVAVVSRDTVKVTAHE
EKLMLVDESATIQCHACTGCGVHLYGRIENNDHAFYGLDFVSTELSKQQGWSAPGFAAFVSSMIETGTPPEQMDGVRVPD
RTRVAAIRLPVAGVDGCPVCACRAQQGSVALMVQPCSRRSGCGVFSS
>Mature_206_residues
THVSIHSSVDGGVARSGAEGFQGGTLKCHCASDKVIVDVSAQTAPNHACGCSKCWKPDGAKFSVVAVVSRDTVKVTAHEE
KLMLVDESATIQCHACTGCGVHLYGRIENNDHAFYGLDFVSTELSKQQGWSAPGFAAFVSSMIETGTPPEQMDGVRVPDR
TRVAAIRLPVAGVDGCPVCACRAQQGSVALMVQPCSRRSGCGVFSS

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 21704; Mature: 21573

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVSIHSSVDGGVARSGAEGFQGGTLKCHCASDKVIVDVSAQTAPNHACGCSKCWKPDG
CCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
AKFSVVAVVSRDTVKVTAHEEKLMLVDESATIQCHACTGCGVHLYGRIENNDHAFYGLDF
CCEEEEEEEECCEEEEEECCCEEEEEECCCEEEEEEECCCCEEEEEEEECCCCEEEEEEH
VSTELSKQQGWSAPGFAAFVSSMIETGTPPEQMDGVRVPDRTRVAAIRLPVAGVDGCPVC
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCEE
ACRAQQGSVALMVQPCSRRSGCGVFSS
EEECCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
THVSIHSSVDGGVARSGAEGFQGGTLKCHCASDKVIVDVSAQTAPNHACGCSKCWKPDG
CEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
AKFSVVAVVSRDTVKVTAHEEKLMLVDESATIQCHACTGCGVHLYGRIENNDHAFYGLDF
CCEEEEEEEECCEEEEEECCCEEEEEECCCEEEEEEECCCCEEEEEEEECCCCEEEEEEH
VSTELSKQQGWSAPGFAAFVSSMIETGTPPEQMDGVRVPDRTRVAAIRLPVAGVDGCPVC
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCEE
ACRAQQGSVALMVQPCSRRSGCGVFSS
EEECCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]