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The map label for this gene is ddl [H]

Identifier: 84625259

GI number: 84625259

Start: 4075219

End: 4076175

Strand: Reverse

Name: ddl [H]

Synonym: XOO_3602

Alternate gene names: 84625259

Gene position: 4076175-4075219 (Counterclockwise)

Preceding gene: 84625260

Following gene: 84625258

Centisome position: 82.51

GC content: 65.1

Gene sequence:

>957_bases
ATGAGCGCGGTCATCGCCAATGCCCGCACCAGCGATCCGGCCATCTTTGGCCGCGTCGCCGTGTTGCTCGGCGGCACTTC
GTCCGAGCGCGAGGTGTCCTTGAACTCCGGCAGCAATGTGCTGGAAGCGCTGCGTGCGCGCGGCGTCGATGCGCAGCCGG
TCGATGGCATTGCGGCACTGGCCAAGGCGTTGGTCGCGCAGCAGTTTGATCGCGTGTTCAATGTGCTGCACGGCCATAAC
GGCGGCGGCGAAGACGGCATCGTGCAGGGCCTGATGGAAGCTTTCGGCGTGCCGTATACCGGCTCGGATGTGCTTGGCTC
GGCGTTGAGCATGGACAAGATCCGTACCAAACAGGTGTGGCTGTCGCTGGGCCTGTCGACACCGCGTTACGCGCGGTTGG
CTGCTGGCGCCAGCGCCGCGGACATTCATGTCGCGGCACAACAGCTCGGTTTACCGGTCATCGTCAAGCCGGCCAATGAA
GGCTCAAGCGTTGGCGTCAGCCGCGTGTTCGACCAGGCACAGCTCGAAGACGCAGTGACCCTGGCTGCCCGCTACGACGG
TGCGCTGTTGATGGAGCAGTTGATCGAAGGCGACGAACTGACCGTTGCGGTGCTCGGCGACACCGCGTTGCCGTCCATCC
GCATCGTGCCCAAGGGCCAGTGGTACGACTACAACGCCAAGTACATCGCCGAAGACACGCAGTATCTGTGCCCGGGCCTG
GAAGGCGAGCCCGAAGCGCAGATCCGCCAACTTGCGCTCGATGCGTTCCGCGCTGCCGGCTGCCGTGGCTGGGGCCGCGT
GGACGTGATGCGCGATGGCAGCAGCGGCCAGCTGTATCTGCTCGAAGTGAACACCGCTCCCGGCATGACCAGCCACTCGC
TGGTGCCCAAGGCCGCCCGCCAGCTCGGCATCGATTTCGAAGAACTGGTCTGGCGCGTGCTGGAGCAGACGCTGTGA

Upstream 100 bases:

>100_bases
ACGTGCTGCAAGACGGTGACCTGCTGTTGATGATGGGCGCCGGCGATATCGGTTATGTGGCACAGCACATCATCAACAAC
GGGTTTGTCGGAGAGCCGGC

Downstream 100 bases:

>100_bases
TGACGGGATTGGGGATTCGGCATTCGGGATTGGTGAACGGCGGTGCCATGCGCGCCTTTGCTGTGGCTGCTGCCGTGACC
AACCCCCAATCTCCAATGCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSAVIANARTSDPAIFGRVAVLLGGTSSEREVSLNSGSNVLEALRARGVDAQPVDGIAALAKALVAQQFDRVFNVLHGHN
GGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAADIHVAAQQLGLPVIVKPANE
GSSVGVSRVFDQAQLEDAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIAEDTQYLCPGL
EGEPEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFEELVWRVLEQTL

Sequences:

>Translated_318_residues
MSAVIANARTSDPAIFGRVAVLLGGTSSEREVSLNSGSNVLEALRARGVDAQPVDGIAALAKALVAQQFDRVFNVLHGHN
GGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAADIHVAAQQLGLPVIVKPANE
GSSVGVSRVFDQAQLEDAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIAEDTQYLCPGL
EGEPEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFEELVWRVLEQTL
>Mature_317_residues
SAVIANARTSDPAIFGRVAVLLGGTSSEREVSLNSGSNVLEALRARGVDAQPVDGIAALAKALVAQQFDRVFNVLHGHNG
GGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAADIHVAAQQLGLPVIVKPANEG
SSVGVSRVFDQAQLEDAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIAEDTQYLCPGLE
GEPEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFEELVWRVLEQTL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=53.7953795379538, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=34.1880341880342, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33744; Mature: 33612

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVIANARTSDPAIFGRVAVLLGGTSSEREVSLNSGSNVLEALRARGVDAQPVDGIAAL
CCCEECCCCCCCCHHHHEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
AKALVAQQFDRVFNVLHGHNGGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVW
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LSLGLSTPRYARLAAGASAADIHVAAQQLGLPVIVKPANEGSSVGVSRVFDQAQLEDAVT
HHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIAEDTQYLCPGL
HHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEECCCCEEEECCCHHHCCCC
EGEPEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAAR
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
QLGIDFEELVWRVLEQTL
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAVIANARTSDPAIFGRVAVLLGGTSSEREVSLNSGSNVLEALRARGVDAQPVDGIAAL
CCEECCCCCCCCHHHHEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
AKALVAQQFDRVFNVLHGHNGGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVW
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LSLGLSTPRYARLAAGASAADIHVAAQQLGLPVIVKPANEGSSVGVSRVFDQAQLEDAVT
HHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIAEDTQYLCPGL
HHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEECCCCEEEECCCHHHCCCC
EGEPEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAAR
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
QLGIDFEELVWRVLEQTL
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]