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The map label for this gene is insI1 [C]

Identifier: 84622565

GI number: 84622565

Start: 994362

End: 995009

Strand: Direct

Name: insI1 [C]

Synonym: XOO_0908

Alternate gene names: 84622565

Gene position: 994362-995009 (Clockwise)

Preceding gene: 84622561

Following gene: 84622567

Centisome position: 20.13

GC content: 62.35

Gene sequence:

>648_bases
ATGCATCTACGCAAACGCCGCCGCAAGCGCCGTCGGCGTGGCGTGCGCGATGGCCGCGGGCAGCTGACGCATCGGCGCAG
CTGGACACAGCGCCCCAGTGTGGTTGAGCAGCGAAGCCGTATCGGCGACTGGGAGCTGGAGACCATCAGGGCCTCACACG
GACGCGCCGTGGTGGTCAGCATGACCGAACGCTGCAGTGGCCTGCATCTGCTGGCTTACTCGCCCGACGGCACCGCCGAG
AACGTGCGCAACGCCATTGTCCAGCGACTGGGCGGCCTGCGCCATGCAGTTCACACCCTCACCGCCGACAACGGCAAGGA
GTTCGCCGATCATCGGCTCATTGCCGCCTGCCTGCAGAGCGATTTCTATTTCGCAGATCCGTACTGCCCATGGCAGCGCG
GCAGCAACGAGAATGCCAACGGATTGACACGCCAATACCTGCCACGGCAGACCGATGTCAGCACCATCACCGATGCGCAC
CTGCGAGATCGAGCAACGGCTCTACAATCGTCCGCGCAAGATACTTGGATTCAAAACGCCCCTCGAAGTCTTCTCCGAGG
AGGCCCTCAACAGCGTTGCGAATCAGAGTTGAATCCGCCCAACATCAGCTACACCGGTGATTCTGGACCCCTGGTTGCAG
TGAAGTAG

Upstream 100 bases:

>100_bases
CGTCAGTCCCGAACAGATCGCCGGTCGCACCGGCTTGGCCAGTCACGCATGAATCTATCGGCACATCGACGCCGACCAGA
AGCGTGGTGGTCAGTTGTTC

Downstream 100 bases:

>100_bases
CAACGCTCCTTGGTAATCGGATCATCGAATGCCAGCGTGTGCGCGAACAGTTGCAGCTGACGCTCCGGGTCGTCGGGCCG
CTGGGCTTAGAGGTCGGGAT

Product: IS1112 transposase

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAE
NVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQSDFYFADPYCPWQRGSNENANGLTRQYLPRQTDVSTITDAH
LRDRATALQSSAQDTWIQNAPRSLLRGGPQQRCESELNPPNISYTGDSGPLVAVK

Sequences:

>Translated_215_residues
MHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAE
NVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQSDFYFADPYCPWQRGSNENANGLTRQYLPRQTDVSTITDAH
LRDRATALQSSAQDTWIQNAPRSLLRGGPQQRCESELNPPNISYTGDSGPLVAVK
>Mature_215_residues
MHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVVSMTERCSGLHLLAYSPDGTAE
NVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQSDFYFADPYCPWQRGSNENANGLTRQYLPRQTDVSTITDAH
LRDRATALQSSAQDTWIQNAPRSLLRGGPQQRCESELNPPNISYTGDSGPLVAVK

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=188, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790736, Length=188, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787671, Length=188, Percent_Identity=27.6595744680851, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 24331; Mature: 24331

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVVS
CCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEEE
MTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQS
ECHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
DFYFADPYCPWQRGSNENANGLTRQYLPRQTDVSTITDAHLRDRATALQSSAQDTWIQNA
CCEECCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRSLLRGGPQQRCESELNPPNISYTGDSGPLVAVK
CHHHHCCCCHHHHHHCCCCCCEEEECCCCCEEEEC
>Mature Secondary Structure
MHLRKRRRKRRRRGVRDGRGQLTHRRSWTQRPSVVEQRSRIGDWELETIRASHGRAVVVS
CCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEEE
MTERCSGLHLLAYSPDGTAENVRNAIVQRLGGLRHAVHTLTADNGKEFADHRLIAACLQS
ECHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
DFYFADPYCPWQRGSNENANGLTRQYLPRQTDVSTITDAHLRDRATALQSSAQDTWIQNA
CCEECCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRSLLRGGPQQRCESELNPPNISYTGDSGPLVAVK
CHHHHCCCCHHHHHHCCCCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]