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The map label for this gene is thiC [H]

Identifier: 84622524

GI number: 84622524

Start: 945550

End: 947427

Strand: Direct

Name: thiC [H]

Synonym: XOO_0867

Alternate gene names: 84622524

Gene position: 945550-947427 (Clockwise)

Preceding gene: 84622523

Following gene: 84622525

Centisome position: 19.14

GC content: 61.71

Gene sequence:

>1878_bases
ATGAATGCCGCGCCTACCGTTTTGCAGCAACAAGCCCAGTCGTTGTCCGAGGCTGTCACCCAACCGATTCCCGGTTCACG
CAAGATCTTCGTGCAAGGCTCGCGCGCGGATCTGCAGGTGCCGATGCGCGAGATCGCGTTGACGCGCACGCCCACCTTGT
TCGGTGGCGAAGAGAATCCGCCGCTCAGCGTCTACGACACTTCCGGGCCTTATACAGATCCGCAGGTGGCTATCGATCTG
GCCGTTGGTCTGGCACCGCTGCGCGCGCACTGGATTGCCGAGCGCGGCGACACCGTCGCACTGGATGGGCTGAGCTCCAG
TTTCGGGCGTGGCCGCGAGCACGATGCGCGTCTGGATGCCGTGCGCTTTCCGGCGCGGCGCTTGCCGCGGGTCGCGCGCG
AGGGCGCCAACGTCACCCAGATGCATTACGCACGCCGCGGCATCATCACGCCGGAAATGGAGTACGTGGCGATCCGCGAA
AATCAGCGTCTGGAAGCGGTGACCGATGCGAGCTTGCGTAAGCAGCATCCGGGCGAGGCGTTCGGCGCCTCGATCCAGCA
GCGCATCACGCCGGAGTTCGTACGCGAGGAGATCGCACGGGGGCGCGCGATCCTGCCCAACAACATCAATCATCCTGAAA
GCGAGCCGATGATCATCGGTCGCAATTTCCTCACCAAGATCAACGCCAACATCGGCAACAGCGCCGTGTCTTCGGGCATT
GCCGAAGAAGTCGAGAAGCTGGTGTGGTCGATCCGCTGGGGCGGCGATACGGTGATGGATCTGTCCACCGGCAAGCACAT
CCATGAAACGCGCGAATGGATCATCCGCAACTCGCCGGTGCCGATCGGTACAGTGCCGATCTATCAGGCATTGGAAAAAG
TCGACGGACGTGCCGAGGCGCTGACCTGGGAAATTTTCCGCGACACCTTGATCGAACAGGCCGAACAGGGCGTGGATTAC
TTCACCATCCACGCCGGCGTACTGTTGCGCTACGTACCGCTGACCGCAAAGCGGGTGACCGGCATCGTGTCGCGTGGCGG
TTCTATCATGGCCAAGTGGTGCCTGGCGCATCACAAGGAAAATTTTCTCTATACGCACTTCGAAGACATCTGCCAGATCA
TGAAGGCCTACGACGTGGCGTTCTCGTTGGGCGATGGCTTACGCCCCGGCTGCATTGCCGACGCGAATGACGCGGCCCAA
TTCGGCGAGCTGGAAACGCTGGGCGAGTTGACCAAACTGGCGTGGAAGCACGATGTGCAAACCATGATCGAAGGCCCCGG
TCATGTGCCGATGCAATTGATCAAGGAGAACATGGACAAGCAGCTGCGCGAATGCGGCGAGGCGCCGTTTTACACCCTGG
GGCCGCTGACCACCGACATCGCGCCGGGCTACGACCACATCACCAGCGCGATCGGTGCGGCAATGATCGGCTGGTTCGGC
ACGGCAATGCTCTGCTATGTCACTCCGAAAGAGCATCTGGGTTTGCCCAATCGGCAGGACGTGCGCGACGGCATCATGGC
CTACAAGATCGCCGCGCACGCAGCCGACCTCGCCAAGGGGCATCCGGGCGCGCAGGTGCGCGACAACGCCCTGAGCAAGG
CGCGTTTCGAGTTCCGTTGGGATGACCAGTTCCATCTCGGTCTGGATCCGGAAAAGGCCAAGGAGTTCCACGACGAGACG
CTGCCCAAGGACGCGCACAAGCTGGCGCACTTCTGTTCGATGTGCGGGCCGCATTTCTGCTCGATGAAGATCACACAGGA
CGTACGCGATTACGCGGCCGAGCACGGAATGGACGACGCGCAAGCGTTATCGACAGGAATGCAGGAGAAGTCGGCGCAGT
TTCTGGCGCAAGGTGCGCAGGTGTATCGCCCCATGTGA

Upstream 100 bases:

>100_bases
AGATGCGATGACCCGTGCGTGCACGGGTCATCGGTATCGAACGCCGCCGCTTCGTCCGCACTGCGACTTTCTCGCAGCGC
CAGCCATTAGGACGAATGCC

Downstream 100 bases:

>100_bases
CGGGTTACATCCGGGCGTTTCAGAAGCGCCACACAGCGCCTGCTTAGGAAAAATTCAGTTTGGTGTGGGGCAGATCGCAT
CGCGGCGCACACGTGCCGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPQVAIDL
AVGLAPLRAHWIAERGDTVALDGLSSSFGRGREHDARLDAVRFPARRLPRVAREGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGEAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMDDAQALSTGMQEKSAQFLAQGAQVYRPM

Sequences:

>Translated_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPQVAIDL
AVGLAPLRAHWIAERGDTVALDGLSSSFGRGREHDARLDAVRFPARRLPRVAREGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGEAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMDDAQALSTGMQEKSAQFLAQGAQVYRPM
>Mature_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPQVAIDL
AVGLAPLRAHWIAERGDTVALDGLSSSFGRGREHDARLDAVRFPARRLPRVAREGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRKQHPGEAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDET
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMDDAQALSTGMQEKSAQFLAQGAQVYRPM

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=69.0280065897858, Blast_Score=887, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69228; Mature: 69228

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPQVAIDLAVGLAPLRAHWIAERGDTVALDGLSSSFGRGREHDARLDA
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCHHHH
VRFPARRLPRVAREGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRKQHPGEA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEA
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWKHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDETLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMDDA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHH
QALSTGMQEKSAQFLAQGAQVYRPM
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPQVAIDLAVGLAPLRAHWIAERGDTVALDGLSSSFGRGREHDARLDA
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCHHHH
VRFPARRLPRVAREGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRKQHPGEA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEA
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWKHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDETLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMDDA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHH
QALSTGMQEKSAQFLAQGAQVYRPM
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]