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The map label for this gene is leuB [H]

Identifier: 84622517

GI number: 84622517

Start: 937103

End: 938176

Strand: Direct

Name: leuB [H]

Synonym: XOO_0860

Alternate gene names: 84622517

Gene position: 937103-938176 (Clockwise)

Preceding gene: 84622516

Following gene: 84622523

Centisome position: 18.97

GC content: 63.78

Gene sequence:

>1074_bases
ATGAGCAAGCAGATTCTGATTCTTCCCGGCGACGGGATCGGGCCGGAGATCATGGCCGAAGCGGTCAAGGTGCTACAGCG
CATTGATACGCAGCACGGCCTGGGTTTCGAGCTGGTGTACGACGAGCTGGGCGGTGCGGCCTACGACAAATACGGCAGCC
CCCTGGCCGATGAAACGCTGGAGCGTGCGCGTGCTGCCGATGCGGTGCTGCTGGGCGCGGTGGGTGGCCCGCAGTGGGAC
ACCATCGACCCGTCGCTACGCCCGGAACGTGGTCTGCTCAAGATCCGCTCGCAGTTGGGGTTGTTCGCCAATCTGCGTCC
GGCACTGTTGTATCCGCAGTTGGCCGACGCCTCCACGCTCAAGCCGGAGGTGGTGGCCGGGCTGGATCTGCTGATCCTGC
GTGAGCTCACCGGCGGCATCTACTTTGGCCAGCCGCGCGGCAATCGCACGCTGGACAATGGCGAGCGCCAGGCCTACGAC
ACCTTGCCCTATAGCGAAAGCGAGATCTGCCGCATCGCCAAGGCAGGCTTCGAGATGGCGCGTTTGCGCGGCAAGAAGTT
GTGTTCGGTCGACAAGGCCAACGTGCTGGCATCGAGCCAGCTGTGGCGTGCAGTGGTCGAAGAAGTGGCCAAGGACTACC
CGGATATCGCGCTGTCGCATATGTACGTGGACAACGCCGCGATGCAGCTGGTGCGCGCGCCCAAGCAGTTCGACGTGATC
GTGACCGACAACATGTTCGGTGACATCCTCTCCGACCAGGCATCCATGCTGACCGGCTCGATCGGCATGCTGCCTTCCGC
CTCGCTGGATGCGAACAGCAAAGGCATGTACGAGCCGTGCCATGGTTCGGCACCGGACATCGCCGGCAAGGGCATTGCCA
ATCCGCTGGCGACGATCCTGTCGGTGGCGATGATGCTGCGCTACACCTTCGCGCAGGCCGATGCAGCGGATGCAATTGAG
CGCGCAGTCGGCAAGGTGCTCGACCAAGGCCTGCGGACTGCCGATATCTGGTCGGAAGGCACCACCAAGGTGGGCACCGT
GGCGATGGGCGATGCGGTGGTGGCTGCGCTGTAA

Upstream 100 bases:

>100_bases
TTTCCAGATCGATGCCGATGGCTCTTTCAGCACCGACGTGCTGGTGCTTTGGGCAGTACGTTGATTCCAATGACGCTGCG
GCGTTACTCACGAGGATGGC

Downstream 100 bases:

>100_bases
AAGCGCTCGCTTCCGCGAGGCGTCGTGGCTGTACCGGTGGCTATCGTTCTTCGGACGATAGCCACTCTCGTTTGGCTGAC
CGGTGTGTGATCACTCATTC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKQILILPGDGIGPEIMAEAVKVLQRIDTQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWD
TIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEICRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIE
RAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Sequences:

>Translated_357_residues
MSKQILILPGDGIGPEIMAEAVKVLQRIDTQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWD
TIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEICRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIE
RAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
>Mature_356_residues
SKQILILPGDGIGPEIMAEAVKVLQRIDTQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWDT
IDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDT
LPYSESEICRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVIV
TDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIER
AVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=32.4242424242424, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=30.2052785923754, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178816, Length=360, Percent_Identity=27.2222222222222, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.6636085626911, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178821, Length=311, Percent_Identity=28.2958199356913, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=140, Percent_Identity=38.5714285714286, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=52.6610644257703, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=35.4929577464789, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=23.8805970149254, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=32.0809248554913, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=30.4597701149425, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=31.7415730337079, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=343, Percent_Identity=30.6122448979592, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=50.1408450704225, Blast_Score=311, Evalue=7e-86,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=36.4406779661017, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=31.1614730878187, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=30.0578034682081, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=33.2335329341317, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=33.2335329341317, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=32.3615160349854, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161078633, Length=340, Percent_Identity=27.3529411764706, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24650122, Length=340, Percent_Identity=27.3529411764706, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=27.3809523809524, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=27.5449101796407, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078635, Length=340, Percent_Identity=27.3529411764706, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=29.0960451977401, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=29.0960451977401, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=26.0233918128655, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38196; Mature: 38065

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILPGDGIGPEIMAEAVKVLQRIDTQHGLGFELVYDELGGAAYDKYGSPLADETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHHCCCCHHHHHH
ERARAADAVLLGAVGGPQWDTIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEICRIAKAGFEMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC
>Mature Secondary Structure 
SKQILILPGDGIGPEIMAEAVKVLQRIDTQHGLGFELVYDELGGAAYDKYGSPLADETL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHHCCCCHHHHHH
ERARAADAVLLGAVGGPQWDTIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEICRIAKAGFEMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]