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The map label for this gene is mii [H]

Identifier: 84622479

GI number: 84622479

Start: 891613

End: 892812

Strand: Direct

Name: mii [H]

Synonym: XOO_0822

Alternate gene names: 84622479

Gene position: 891613-892812 (Clockwise)

Preceding gene: 84622478

Following gene: 84622481

Centisome position: 18.05

GC content: 65.83

Gene sequence:

>1200_bases
ATGCGCCATCTTCCCCAACTCCGCATCCCCGCCACCTACATGCGCGGTGGTACCAGCAAGGGCGTGTTTTTCCGTCTGCA
GGATCTGCCCGTGGCCGCGCAGCAGCCGGGCGCTGCACGCGATGCGTTGTTGTTGCGGGTGGTCGGCAGCCCGGATCCGT
ATGCCAAGCAGATCGACGGCATGGGCGGTGCCACTTCGAGCACCAGCAAGAGCGTGATCGTGTCGGCCAGCACGCGCGAT
GGGCATGATGTGGACTATCTGTTCGGGCAGGTCTCCATCGATAGCGCGTTCGTGGATTGGAGCGGCAACTGCGGCAATCT
GTCGGCGGCGGTGGGGCCATTCGCCATCGCCAGCGGCTTGATCGATCCCGCACGCGTGCAACGCGATGGCGTGGCGACGG
TGCGGATCTGGCAGGCCAATATCGGCAAGACGATTGTCGCGCATGTGCCGATGACCGATGGGGCGGTGCAGGAGACCGGC
GACTTCGAACTGGACGGCGTAACGTTCCCGGCGGCCGAAGTCCAGCTGGAATTTCTCGACCCGGCCGACGATGCCGAGGG
CGAGGGCGGGGCGATGTTTCCCACCGGCAATGTCATCGATCAACTGGAGATCCCCGGGCTGGGCACGCTCGATGCCACGC
TGATCAATGCGGGCATTCCAACCATCTTCCTCAACGCACGCGACCTGGGTTACACCGGCACCGAGCTGCAGGACGCCATC
AATGGCGACCCGCGCGCCTTGATCATGTTCGAAACATTGCGCGCGCACGGCGCAGTGCGGATGGGCTTGATTGCGAAGGT
GGAAGACGCTGCGACGCGGCAGCACACGCCCAAGGTCGCCTTCGTGGCACCGCCGGCCGACTACACCGCGTCCAGCGGCA
AGCCGGTGCATTCGGCCGAAATCGATTTGCTGGTGCGCGCAATGTCGATGGGCAAATTGCATCACGCGATGATGGGCACT
GCAGCAGTGGCCATCGGCACCGCGGCAGCGGTGCCGGGCACGCTGGTCAATCTGGCTGCCGGCGGCGCGGCGCGTTCGGC
GGTGCGTTTCGGGCATCCGTCCGGCACCTTGCGGGTGGGTGCGCAGGCGCAGTTGGTCGATGGCCAGTGGCAGGTGACCA
AGGCGCTGATGAGCCGCAGCGCGCGGGTGCTGATGGAAGGCTGGGTGCGGGTGCCGGCTCCTGTTGCCGAGGTTCAGTGA

Upstream 100 bases:

>100_bases
ACAATGAACTGCATAACGCACGCGTAGCGCTGAGCGCTTGCACTGCTGCAATCGCTATCCCCTTTGCTGCGACTCTGTCC
AGTGAGCTAACCGAGGTTCC

Downstream 100 bases:

>100_bases
CGCGCACCGGGTGCGGGACTGCTTGGATGCACGCTGCGCACCGGTCGTCGTGGACATGCATTCGCGATGTTGCGTGCGGT
GTCTGCATCGCTTTGATCGG

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRHLPQLRIPATYMRGGTSKGVFFRLQDLPVAAQQPGAARDALLLRVVGSPDPYAKQIDGMGGATSSTSKSVIVSASTRD
GHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVQRDGVATVRIWQANIGKTIVAHVPMTDGAVQETG
DFELDGVTFPAAEVQLEFLDPADDAEGEGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFLNARDLGYTGTELQDAI
NGDPRALIMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSGKPVHSAEIDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVDGQWQVTKALMSRSARVLMEGWVRVPAPVAEVQ

Sequences:

>Translated_399_residues
MRHLPQLRIPATYMRGGTSKGVFFRLQDLPVAAQQPGAARDALLLRVVGSPDPYAKQIDGMGGATSSTSKSVIVSASTRD
GHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVQRDGVATVRIWQANIGKTIVAHVPMTDGAVQETG
DFELDGVTFPAAEVQLEFLDPADDAEGEGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFLNARDLGYTGTELQDAI
NGDPRALIMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSGKPVHSAEIDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVDGQWQVTKALMSRSARVLMEGWVRVPAPVAEVQ
>Mature_399_residues
MRHLPQLRIPATYMRGGTSKGVFFRLQDLPVAAQQPGAARDALLLRVVGSPDPYAKQIDGMGGATSSTSKSVIVSASTRD
GHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGLIDPARVQRDGVATVRIWQANIGKTIVAHVPMTDGAVQETG
DFELDGVTFPAAEVQLEFLDPADDAEGEGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFLNARDLGYTGTELQDAI
NGDPRALIMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSGKPVHSAEIDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVGAQAQLVDGQWQVTKALMSRSARVLMEGWVRVPAPVAEVQ

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=386, Percent_Identity=33.160621761658, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41620; Mature: 41620

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLPQLRIPATYMRGGTSKGVFFRLQDLPVAAQQPGAARDALLLRVVGSPDPYAKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHCC
MGGATSSTSKSVIVSASTRDGHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHCC
IDPARVQRDGVATVRIWQANIGKTIVAHVPMTDGAVQETGDFELDGVTFPAAEVQLEFLD
CCHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PADDAEGEGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFLNARDLGYTGTELQDAI
CCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
NGDPRALIMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSGKPVHSAE
CCCCCEEEEEEEHHHCCCEEEEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHH
IDLLVRAMSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCHHHHEEECCCCCCEEEC
AQAQLVDGQWQVTKALMSRSARVLMEGWVRVPAPVAEVQ
CEEEEECCHHHHHHHHHHCCHHHHHHCCEECCCCHHCCC
>Mature Secondary Structure
MRHLPQLRIPATYMRGGTSKGVFFRLQDLPVAAQQPGAARDALLLRVVGSPDPYAKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCHHHHHHCC
MGGATSSTSKSVIVSASTRDGHDVDYLFGQVSIDSAFVDWSGNCGNLSAAVGPFAIASGL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHCC
IDPARVQRDGVATVRIWQANIGKTIVAHVPMTDGAVQETGDFELDGVTFPAAEVQLEFLD
CCHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PADDAEGEGGAMFPTGNVIDQLEIPGLGTLDATLINAGIPTIFLNARDLGYTGTELQDAI
CCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
NGDPRALIMFETLRAHGAVRMGLIAKVEDAATRQHTPKVAFVAPPADYTASSGKPVHSAE
CCCCCEEEEEEEHHHCCCEEEEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHH
IDLLVRAMSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGAARSAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCHHHHEEECCCCCCEEEC
AQAQLVDGQWQVTKALMSRSARVLMEGWVRVPAPVAEVQ
CEEEEECCHHHHHHHHHHCCHHHHHHCCEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA