Click here to switch to the map view.

The map label for this gene is prpC [H]

Identifier: 84622475

GI number: 84622475

Start: 886954

End: 888108

Strand: Direct

Name: prpC [H]

Synonym: XOO_0818

Alternate gene names: 84622475

Gene position: 886954-888108 (Clockwise)

Preceding gene: 84622474

Following gene: 84622476

Centisome position: 17.95

GC content: 60.78

Gene sequence:

>1155_bases
ATGAACGACACTGTCAAGCCTGGTTTCAAGCCGAAAAAATCCGTTGCCTTGTCCGGTACCGCTGCCGGCAACACCGCGCT
GTGCACGGTGGGGCGCAGTGGCAACGATCTGCATTACCGCGGCTACGACATTCTCGATCTGGCCACCACCAGCGAGTTCG
AAGAGATTGCGTATCTGTTGGTACACGGCAAGCTGCCCAACAGCGGCGAACTGCGTGGTTACAAGGCCAAGCTGCGCTCG
CTGCGCGGTGTGCCGGCTGCAGTGAAAGCTGCGCTGGAACAGTTGCCGCCGTCCGCGCATCCGATGGATGTGATGCGTAC
CGGCGTGTCGGTGCTGGGCTGCCTGCAGCCGGAAAAAGACGACCACAATCATCCTGGCGCGCGCGACATCGCCGACAAGC
TGATGGCGTCATTGGGTTCGATGCTGCTGTACTGGTATCACTACAGCCACAACGGCAAGCGCATCGACGTGGAAACCGAC
GACGACTCGATCGGCGGCCATTTCCTGCATCTGCTGCATGGCTTTGCACCGAAAGACGCGTGGGTGCGCGCGATGCATAC
CAGCCTGATCTTGTATGCCGAGCACGAGTTCAATGCGTCCACGTTTGCGAGCCGGGTGATTGCCGGTACCGGCAGCGATA
TATACAGCGCCATCGCCGGTGGTATCGGCGCATTGCGCGGGCCAAAACATGGCGGCGCCAATGAAGTGGCCTTTGAGGTG
CAGAAGCGCTACGACACCCCGGATGAAGCAGAAGCCGATATCAAGGCGCGCGTGGAGCGCAAGGAAGTGGTGATCGGCTT
CGGGCACCCGGTGTACACGCTGTCCGACCCGCGCAACAAGGTGATCAAGGACGTGGCGCGCGAGCTCTCCGATGAGCAGG
GCAGCCGCAAGATGTACGACATCGCCGAGCGTCTGGAGAGCGTCATGTGGGACATCAAGAAGATGTTCCCGAACCTGGAT
TGGTTCAGTGCGGTGAGCTACCACATGATGGGCGTACCGACGGCAATGTTCACCCCGCTGTTCGTGCTTGCGCGCACTGC
TGGTTGGAGCGCGCACATCATCGAGCAGCGCGTGGACGGCAAGATCATCCGTCCCTCGGCCAATTACACCGGCCCGGAAG
ATCAGGTGTTCGTGCCGTTGGATCAACGCCGCTGA

Upstream 100 bases:

>100_bases
GTTGTTCGCGCGCAAAGGCGCATGATTACGGGTTGCTGATCATCTTCATTGTCGCCTTTTCAGCCACACCGCGACACGAC
ACAGTTTGGGAGCTCATCGC

Downstream 100 bases:

>100_bases
GGCAGCCGAGGATGCCTTGCGCAGCCGCCGACTTGTGCGCTTCGGTCGCCTTCTTCAACGAACGCGATTGGATCTCGCAA
GCCAGATCAGCGACAGCACG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNDTVKPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATTSEFEEIAYLLVHGKLPNSGELRGYKAKLRS
LRGVPAAVKAALEQLPPSAHPMDVMRTGVSVLGCLQPEKDDHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIDVETD
DDSIGGHFLHLLHGFAPKDAWVRAMHTSLILYAEHEFNASTFASRVIAGTGSDIYSAIAGGIGALRGPKHGGANEVAFEV
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTLSDPRNKVIKDVARELSDEQGSRKMYDIAERLESVMWDIKKMFPNLD
WFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDGKIIRPSANYTGPEDQVFVPLDQRR

Sequences:

>Translated_384_residues
MNDTVKPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATTSEFEEIAYLLVHGKLPNSGELRGYKAKLRS
LRGVPAAVKAALEQLPPSAHPMDVMRTGVSVLGCLQPEKDDHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIDVETD
DDSIGGHFLHLLHGFAPKDAWVRAMHTSLILYAEHEFNASTFASRVIAGTGSDIYSAIAGGIGALRGPKHGGANEVAFEV
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTLSDPRNKVIKDVARELSDEQGSRKMYDIAERLESVMWDIKKMFPNLD
WFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDGKIIRPSANYTGPEDQVFVPLDQRR
>Mature_384_residues
MNDTVKPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATTSEFEEIAYLLVHGKLPNSGELRGYKAKLRS
LRGVPAAVKAALEQLPPSAHPMDVMRTGVSVLGCLQPEKDDHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIDVETD
DDSIGGHFLHLLHGFAPKDAWVRAMHTSLILYAEHEFNASTFASRVIAGTGSDIYSAIAGGIGALRGPKHGGANEVAFEV
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTLSDPRNKVIKDVARELSDEQGSRKMYDIAERLESVMWDIKKMFPNLD
WFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDGKIIRPSANYTGPEDQVFVPLDQRR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=376, Percent_Identity=75.7978723404255, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1786939, Length=389, Percent_Identity=31.1053984575836, Blast_Score=182, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324328, Length=345, Percent_Identity=25.5072463768116, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=344, Percent_Identity=24.1279069767442, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24640124, Length=344, Percent_Identity=23.8372093023256, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640126, Length=344, Percent_Identity=23.8372093023256, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42375; Mature: 42375

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTVKPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATTSEFEEIAYLL
CCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCEEEEECCHHHHHHHEEHE
VHGKLPNSGELRGYKAKLRSLRGVPAAVKAALEQLPPSAHPMDVMRTGVSVLGCLQPEKD
EECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
DHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIDVETDDDSIGGHFLHLLHGFAPKDA
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHH
WVRAMHTSLILYAEHEFNASTFASRVIAGTGSDIYSAIAGGIGALRGPKHGGANEVAFEV
HHHHHHHEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCHHHHHH
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTLSDPRNKVIKDVARELSDEQGSRKMYD
HHHCCCCCCCCCHHHHHCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHH
IAERLESVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC
KIIRPSANYTGPEDQVFVPLDQRR
EEECCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MNDTVKPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATTSEFEEIAYLL
CCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCEEEEECCHHHHHHHEEHE
VHGKLPNSGELRGYKAKLRSLRGVPAAVKAALEQLPPSAHPMDVMRTGVSVLGCLQPEKD
EECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
DHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIDVETDDDSIGGHFLHLLHGFAPKDA
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHH
WVRAMHTSLILYAEHEFNASTFASRVIAGTGSDIYSAIAGGIGALRGPKHGGANEVAFEV
HHHHHHHEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCHHHHHH
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTLSDPRNKVIKDVARELSDEQGSRKMYD
HHHCCCCCCCCCHHHHHCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHH
IAERLESVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC
KIIRPSANYTGPEDQVFVPLDQRR
EEECCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]