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The map label for this gene is prpB [H]

Identifier: 84622474

GI number: 84622474

Start: 885982

End: 886878

Strand: Direct

Name: prpB [H]

Synonym: XOO_0816

Alternate gene names: 84622474

Gene position: 885982-886878 (Clockwise)

Preceding gene: 84622472

Following gene: 84622475

Centisome position: 17.93

GC content: 66.0

Gene sequence:

>897_bases
ATGACGCCTTCGTCCCCCTCTTCGGCCGGCACGCGCTTCCGCGCCGCGCTGGCTGCCGAATCGCCGCTGCAGGTGATCGG
CGCGATCAACGCCAACCATGCGCTGTTGGCTCAGCGCGCCGGCTACCAGGCGATTTACCTGTCTGGCGGCGGCGTGGCGG
CCGGGTCGCTGGGGTTGCCGGACCTGGGCATCAACACGCTGGAAGACGTGCTGATCGACGTCCGCCGTATCACCGACGTG
TGCGAGCTGCCGCTGCTGGTGGACGTGGACACCGGCTTCGGGCCGAGCGCGTTCAACATCGAGCGCACCATCAAATCGCT
GATCAAGGCGGGCGCGGGCGGTTGCCATATCGAAGACCAGGTGGGCGCCAAGCGCTGCGGCCACCGGCCGGGCAAGGAGA
TCGTGAGCCAGGGTGAGATGGTGGACCGGGTCAAGGCGGCAGCCGATGCCAAGACCGACGCGGCGTTCTTCCTGATCGCC
CGCACCGACGCGATCCAGATGGAAGGCGTGGATGCGGCGATCGAACGCGCCATCGCCTGCGTGGAAGCGGGTGCGGATGG
CATCTTCGCCGAGGCCGCCTACGACCTGGAAACCTACAAGCGTTTTGTCGATGCGGTCAACGTGCCGGTGCTGGCCAACA
TCACCGAGTTCGGCAAGACCCCGCTGTTTACGCGCGACCAGCTGGCGCAGGCCGGCGTGGCGATTCAGTTGTTTCCGCTG
TCGGCGTTTCGTGCCGCCAACAAGGCCGCTGAGGCGGTTTACACGGCGATCCGTCGCGAAGGTCATCAACAAGCCGTGCT
GGACAGCATGCAGACGCGCGAAGAGCTGTACGAACGCATCGGTTACCACGACTACGAGCAGCGCCTGGATGCGTTGTTCG
CGCGCAAAGGCGCATGA

Upstream 100 bases:

>100_bases
CGCACTTGCAACATCTGGAGCGGCGAGGTCTAAAAGAATCAACGGCGTGTGATTGGCACGGTGTTTGCGCCTACAGCGAT
ATTCCACCCAAGGAACCGCC

Downstream 100 bases:

>100_bases
TTACGGGTTGCTGATCATCTTCATTGTCGCCTTTTCAGCCACACCGCGACACGACACAGTTTGGGAGCTCATCGCATGAA
CGACACTGTCAAGCCTGGTT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTPSSPSSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDV
CELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMVDRVKAAADAKTDAAFFLIA
RTDAIQMEGVDAAIERAIACVEAGADGIFAEAAYDLETYKRFVDAVNVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
SAFRAANKAAEAVYTAIRREGHQQAVLDSMQTREELYERIGYHDYEQRLDALFARKGA

Sequences:

>Translated_298_residues
MTPSSPSSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDV
CELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMVDRVKAAADAKTDAAFFLIA
RTDAIQMEGVDAAIERAIACVEAGADGIFAEAAYDLETYKRFVDAVNVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
SAFRAANKAAEAVYTAIRREGHQQAVLDSMQTREELYERIGYHDYEQRLDALFARKGA
>Mature_297_residues
TPSSPSSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDVC
ELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMVDRVKAAADAKTDAAFFLIAR
TDAIQMEGVDAAIERAIACVEAGADGIFAEAAYDLETYKRFVDAVNVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPLS
AFRAANKAAEAVYTAIRREGHQQAVLDSMQTREELYERIGYHDYEQRLDALFARKGA

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=70.446735395189, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1790445, Length=295, Percent_Identity=26.4406779661017, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982926, Length=176, Percent_Identity=32.3863636363636, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=176, Percent_Identity=32.3863636363636, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31766; Mature: 31635

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSSPSSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLP
CCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGINTLEDVLIDVRRITDVCELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQ
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
VGAKRCGHRPGKEIVSQGEMVDRVKAAADAKTDAAFFLIARTDAIQMEGVDAAIERAIAC
HHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCEEEECCHHHHHHHHHHH
VEAGADGIFAEAAYDLETYKRFVDAVNVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECHHHHCCCCCCCHHHHHHCCCEEEEECH
SAFRAANKAAEAVYTAIRREGHQQAVLDSMQTREELYERIGYHDYEQRLDALFARKGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSSPSSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLP
CCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGINTLEDVLIDVRRITDVCELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQ
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
VGAKRCGHRPGKEIVSQGEMVDRVKAAADAKTDAAFFLIARTDAIQMEGVDAAIERAIAC
HHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCEEEECCHHHHHHHHHHH
VEAGADGIFAEAAYDLETYKRFVDAVNVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECHHHHCCCCCCCHHHHHHCCCEEEEECH
SAFRAANKAAEAVYTAIRREGHQQAVLDSMQTREELYERIGYHDYEQRLDALFARKGA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]