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The map label for this gene is rfbD [H]

Identifier: 84622380

GI number: 84622380

Start: 778587

End: 779495

Strand: Direct

Name: rfbD [H]

Synonym: XOO_0723

Alternate gene names: 84622380

Gene position: 778587-779495 (Clockwise)

Preceding gene: 84622379

Following gene: 84622383

Centisome position: 15.76

GC content: 64.25

Gene sequence:

>909_bases
GTGACCACGCTGGTTTTTGGCGCAAATGGGCAGGTGGGTACGGAATTGCTGCGTGCGCTGGCAGTCGATGGCGCCGTACA
GGCCACCACGCGTAGTGGCCAGTTGCCCGACGGCAGCGCGTGTGAAACAGCAGACTTCGATGCGCCTGAGACGCTGACCG
CATTGCTGGATCGAATCAAGCCGTCGCGTGTGGTCAATGCCGCCGCTTACACGGCTGTAGATCGTGCCGAGCAGGATCGG
GAACGCGCGACCCGTGCCAATGCGACGGCACCCGGCGTCGTCGCAGCCTGGTGCGCGTCCAATCGTGTGCCGCTTGTGCA
TTACTCCACCGATTACGTCTTCGACGGGCAGGGCGCAGCGCCATATCGCGAGAACGCGCAGACGTCGCCACTCGGCGTGT
ACGGCGAAACCAAGCTTGCCGGCGAGCAGGCCATCCGTGCCTCCGGCGCCCAGCATCTGATCCTGCGGACTGCTTGGGTC
TACGCCTCGCATGGCGCGAACTTCCTGCGCACGATGTTGCGGGTAGGGGCGGAGCGGGACGAGTTGCGTGTGGTGGCCGA
TCAGATCGGAACACCGACGCCGGCGGCATTGATTGCCGATGTCACTGCACAACTGCTGCGTCAGCGCACGCCCGATACAT
CGGGGACCTGGCATCTCACCGCGGCTGGCCAGACCAGCTGGCATGGATTTGCCGAGGCAATCTTCGAAGAGGCGGTCAGT
GCCGGGTTGCTGGAGCGTGCACCGCGCGTGGTGCCGATCACGACGGCCGATTATCCGACGCCAGCCAGGCGTCCGGCATA
TTCGCGGTTGTCCATCGATACACTGCAGGGCGATTTCGGTATCGAGCTGCCTGAGTGGCGCGTTGGCCTGCAGCGTGTGA
TTGCCGAGCTGTCTGCGACGAAGAAGTAG

Upstream 100 bases:

>100_bases
GTATCGATTGGCCGATCAGCAATCCATTGCTCTCTGCAAAGGACGCCGCTGCGCCGTTTCTGGCCGATGTGCCCGCTGAG
CGCTTGCCGGTGTATAAACC

Downstream 100 bases:

>100_bases
CTCGACAGCAGGTCAATGACGACAGCAGTCACTGCTGGCGCCTGATATCCGGAAAGCAAACAGCCGGGAATTCCCGGCTG
TTTGCTTATACGGCGCAACC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTTLVFGANGQVGTELLRALAVDGAVQATTRSGQLPDGSACETADFDAPETLTALLDRIKPSRVVNAAAYTAVDRAEQDR
ERATRANATAPGVVAAWCASNRVPLVHYSTDYVFDGQGAAPYRENAQTSPLGVYGETKLAGEQAIRASGAQHLILRTAWV
YASHGANFLRTMLRVGAERDELRVVADQIGTPTPAALIADVTAQLLRQRTPDTSGTWHLTAAGQTSWHGFAEAIFEEAVS
AGLLERAPRVVPITTADYPTPARRPAYSRLSIDTLQGDFGIELPEWRVGLQRVIAELSATKK

Sequences:

>Translated_302_residues
MTTLVFGANGQVGTELLRALAVDGAVQATTRSGQLPDGSACETADFDAPETLTALLDRIKPSRVVNAAAYTAVDRAEQDR
ERATRANATAPGVVAAWCASNRVPLVHYSTDYVFDGQGAAPYRENAQTSPLGVYGETKLAGEQAIRASGAQHLILRTAWV
YASHGANFLRTMLRVGAERDELRVVADQIGTPTPAALIADVTAQLLRQRTPDTSGTWHLTAAGQTSWHGFAEAIFEEAVS
AGLLERAPRVVPITTADYPTPARRPAYSRLSIDTLQGDFGIELPEWRVGLQRVIAELSATKK
>Mature_301_residues
TTLVFGANGQVGTELLRALAVDGAVQATTRSGQLPDGSACETADFDAPETLTALLDRIKPSRVVNAAAYTAVDRAEQDRE
RATRANATAPGVVAAWCASNRVPLVHYSTDYVFDGQGAAPYRENAQTSPLGVYGETKLAGEQAIRASGAQHLILRTAWVY
ASHGANFLRTMLRVGAERDELRVVADQIGTPTPAALIADVTAQLLRQRTPDTSGTWHLTAAGQTSWHGFAEAIFEEAVSA
GLLERAPRVVPITTADYPTPARRPAYSRLSIDTLQGDFGIELPEWRVGLQRVIAELSATKK

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=186, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI11034825, Length=186, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788352, Length=304, Percent_Identity=46.7105263157895, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32339; Mature: 32207

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLVFGANGQVGTELLRALAVDGAVQATTRSGQLPDGSACETADFDAPETLTALLDRIK
CCEEEECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
PSRVVNAAAYTAVDRAEQDRERATRANATAPGVVAAWCASNRVPLVHYSTDYVFDGQGAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEECCCCCC
PYRENAQTSPLGVYGETKLAGEQAIRASGAQHLILRTAWVYASHGANFLRTMLRVGAERD
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHHCCCHH
ELRVVADQIGTPTPAALIADVTAQLLRQRTPDTSGTWHLTAAGQTSWHGFAEAIFEEAVS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
AGLLERAPRVVPITTADYPTPARRPAYSRLSIDTLQGDFGIELPEWRVGLQRVIAELSAT
HHHHHHCCCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
KK
CC
>Mature Secondary Structure 
TTLVFGANGQVGTELLRALAVDGAVQATTRSGQLPDGSACETADFDAPETLTALLDRIK
CEEEECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
PSRVVNAAAYTAVDRAEQDRERATRANATAPGVVAAWCASNRVPLVHYSTDYVFDGQGAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEECCCCCC
PYRENAQTSPLGVYGETKLAGEQAIRASGAQHLILRTAWVYASHGANFLRTMLRVGAERD
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHHCCCHH
ELRVVADQIGTPTPAALIADVTAQLLRQRTPDTSGTWHLTAAGQTSWHGFAEAIFEEAVS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
AGLLERAPRVVPITTADYPTPARRPAYSRLSIDTLQGDFGIELPEWRVGLQRVIAELSAT
HHHHHHCCCEEEEECCCCCCCCCCCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]