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The map label for this gene is rmlA [H]

Identifier: 84622378

GI number: 84622378

Start: 777149

End: 778036

Strand: Direct

Name: rmlA [H]

Synonym: XOO_0721

Alternate gene names: 84622378

Gene position: 777149-778036 (Clockwise)

Preceding gene: 84622377

Following gene: 84622379

Centisome position: 15.73

GC content: 56.98

Gene sequence:

>888_bases
ATGACGCAACGTAAAGGCATTATTTTGGCCGGTGGCTCGGGGACCCGCCTGTATCCGATCACCAAGGGTGTCAGTAAGCA
ACTGCTGCCGGTGTACGACAAGCCGATGATCTATTACCCGCTGAGCGTCCTGATGCTGGCAGGTATCCGCGATATTTTGA
TCATCAATACGCCGCATGAGCAGGCGTTGTTTCAATCCTTGTTGGGTGATGGCTCTCAGTGGGGAATTCACATTCAATAC
GCCGTGCAGCCCAGCCCGGACGGGTTGGCGCAGGCGTATTTGATCGGTCGCGACTTTGTCGATGGCAAGCCGAGTTGCCT
GGTATTGGGCGACAATATTTTTCATGGTCACGGTTTGACCGAAACGTTACGCCGCGCAGACGCGCGCGAGCAGGGCGCGA
CCGTGTTCGGTTATTGGGTCACCGATCCCGAGCGCTACGGCGTGGCCGAATTCGACCAGCAAGGCAAAGTGATCGATATC
GCCGAAAAGCCTGAGAAGCCGCGCTCCAACTATGCGGTCACCGGCCTGTACTTCTACGATGGAAAAGCCAGCGATTATGC
GGCTGCCTTGAAACCTTCTCCGCGTGGCGAGCTGGAGATTACCGATCTCAATCGCTGCTATCTCGATGCTGGCGATCTGC
ATCTTGAGCCGCTGGGACGCGGGTATGCATGGCTGGATACCGGGACCCACCAGTCCTTGCACGAAGCGGCCAACTTCATC
GAGACCATCCAGATGCGTCAGGGCCTGCAGGTGTGCTGCCCCGAAGAAATCGCGTTCGGTCAGGGCTGGATCGATGCGGA
GCAATTGGAGCGTCTGGCCGCCCCGTTGCTGAAAAACGACTATGGCAAATATTTGCACGAATTGGCTGTGCGAGGAGTAG
TACCGTGA

Upstream 100 bases:

>100_bases
CTGGATGGCAGTTACCGGTTGGAACGGATCGGCGCCTCGGCGTAACGCGCCACCGCAAGAGCCCGCGCAACCGTGTGTGG
GCCTCACAAGGAACTATCTC

Downstream 100 bases:

>100_bases
AAATCGTCGAAACGCGTTTACCAGGTTGTGTCGTCATCGAGCCGGCAGTGTTCGGCGATGAGCGCGGATATTTCTTCGAA
ACGTGGAATGCCGAGCGCTT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTQRKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILIINTPHEQALFQSLLGDGSQWGIHIQY
AVQPSPDGLAQAYLIGRDFVDGKPSCLVLGDNIFHGHGLTETLRRADAREQGATVFGYWVTDPERYGVAEFDQQGKVIDI
AEKPEKPRSNYAVTGLYFYDGKASDYAAALKPSPRGELEITDLNRCYLDAGDLHLEPLGRGYAWLDTGTHQSLHEAANFI
ETIQMRQGLQVCCPEEIAFGQGWIDAEQLERLAAPLLKNDYGKYLHELAVRGVVP

Sequences:

>Translated_295_residues
MTQRKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILIINTPHEQALFQSLLGDGSQWGIHIQY
AVQPSPDGLAQAYLIGRDFVDGKPSCLVLGDNIFHGHGLTETLRRADAREQGATVFGYWVTDPERYGVAEFDQQGKVIDI
AEKPEKPRSNYAVTGLYFYDGKASDYAAALKPSPRGELEITDLNRCYLDAGDLHLEPLGRGYAWLDTGTHQSLHEAANFI
ETIQMRQGLQVCCPEEIAFGQGWIDAEQLERLAAPLLKNDYGKYLHELAVRGVVP
>Mature_294_residues
TQRKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILIINTPHEQALFQSLLGDGSQWGIHIQYA
VQPSPDGLAQAYLIGRDFVDGKPSCLVLGDNIFHGHGLTETLRRADAREQGATVFGYWVTDPERYGVAEFDQQGKVIDIA
EKPEKPRSNYAVTGLYFYDGKASDYAAALKPSPRGELEITDLNRCYLDAGDLHLEPLGRGYAWLDTGTHQSLHEAANFIE
TIQMRQGLQVCCPEEIAFGQGWIDAEQLERLAAPLLKNDYGKYLHELAVRGVVP

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=289, Percent_Identity=67.1280276816609, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1790224, Length=284, Percent_Identity=65.1408450704225, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788355, Length=240, Percent_Identity=24.5833333333333, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=190, Percent_Identity=27.3684210526316, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=193, Percent_Identity=29.0155440414508, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355443, Length=227, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644084, Length=227, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32665; Mature: 32533

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILIINTPHE
CCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCHH
QALFQSLLGDGSQWGIHIQYAVQPSPDGLAQAYLIGRDFVDGKPSCLVLGDNIFHGHGLT
HHHHHHHHCCCCCCEEEEEEEECCCCCHHHHEEEEECCCCCCCCCEEEEECCEEECCCHH
ETLRRADAREQGATVFGYWVTDPERYGVAEFDQQGKVIDIAEKPEKPRSNYAVTGLYFYD
HHHHHHHHHHCCCEEEEEEECCCHHCCCHHHCCCCCEEEECCCCCCCCCCEEEEEEEEEC
GKASDYAAALKPSPRGELEITDLNRCYLDAGDLHLEPLGRGYAWLDTGTHQSLHEAANFI
CCCCCEEEECCCCCCCCEEEEECCEEEEECCCEEECCCCCCEEEEECCCHHHHHHHHHHH
ETIQMRQGLQVCCPEEIAFGQGWIDAEQLERLAAPLLKNDYGKYLHELAVRGVVP
HHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQRKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLAGIRDILIINTPHE
CCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCHH
QALFQSLLGDGSQWGIHIQYAVQPSPDGLAQAYLIGRDFVDGKPSCLVLGDNIFHGHGLT
HHHHHHHHCCCCCCEEEEEEEECCCCCHHHHEEEEECCCCCCCCCEEEEECCEEECCCHH
ETLRRADAREQGATVFGYWVTDPERYGVAEFDQQGKVIDIAEKPEKPRSNYAVTGLYFYD
HHHHHHHHHHCCCEEEEEEECCCHHCCCHHHCCCCCEEEECCCCCCCCCCEEEEEEEEEC
GKASDYAAALKPSPRGELEITDLNRCYLDAGDLHLEPLGRGYAWLDTGTHQSLHEAANFI
CCCCCEEEECCCCCCCCEEEEECCEEEEECCCEEECCCCCCEEEEECCCHHHHHHHHHHH
ETIQMRQGLQVCCPEEIAFGQGWIDAEQLERLAAPLLKNDYGKYLHELAVRGVVP
HHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]