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The map label for this gene is rfbB [H]

Identifier: 84622377

GI number: 84622377

Start: 776038

End: 777093

Strand: Direct

Name: rfbB [H]

Synonym: XOO_0720

Alternate gene names: 84622377

Gene position: 776038-777093 (Clockwise)

Preceding gene: 84622372

Following gene: 84622378

Centisome position: 15.71

GC content: 59.85

Gene sequence:

>1056_bases
TTGGCAACTTGGCTTGTAACCGGCGGTGCCGGCTTCATTGGTGGCAACTTTGTTCTTGAGGCAGTGGCTCGTGGGATCCG
CGTGGTCAATCTCGATGCACTGACCTATGCGGGAAACTTGAACACGTTGGCATCCCTGGAAGGCAATCCGGACCACGTTT
TCGTCAAGGGCGATATTGGCGACGGCATGTTGGTCGCCCGTCTGTTGCAGGAACACCAGCCAGACGCGGTGCTGAATTTC
GCAGCCGAGAGCCACGTGGATCGCTCGATCGAAGGTCCGGGCGCATTCATTCACACCAATGTCGTCGGGACTTTAGCGTT
GCTCGAAGCAGTACGCGACTATTGGAAGTCGCTGCCTACCGCGCGCAGCGATGCTTTCCGTTTCCTGCATGTCTCAACCG
ACGAGGTCTACGGCACGCTGGGCGAAACCGGTAAGTTCACCGAGACCACACCTTACGCGCCGAACTCGCCGTATTCGGCC
TCCAAGGCGGCCTCGGATCATCTGGTACGTGCGTTCCACCACACCTACGGCCTGCCGGTGCTGACCACCAATTGTTCGAA
CAATTATGGTCCGTACCACTTCCCCGAAAAGCTCATTCCGCTGGTCATCGCCAAAGCGCTTGCCGGCGAGCCGCTGCCGG
TCTACGGCGACGGCAAGCAGGTGCGCGACTGGTTGTTCGTCAGCGACCACTGCGAAGCGATTCGTACCGTGTTGGCGAAG
GGCAAGGTCGGCGAGACCTACAACGTGGGCGGCAATTCAGAACGCCAGAATATCGAAGTCGTGCAGGCGATCTGCGCCCT
GCTGGATCAGCACCGTCCACGCGACGATGGCAAGCCGCGTGCGAGCCAGATCACCTATGTGACGGATCGCCCGGGCCATG
ATCGCCGCTACGCGATCGACGCTTCCAAGCTGAAGAACGAACTGGGCTGGGAGCCTTCCTACACGTTTGAGCAAGGCATC
GCGCAGACAGTGCAATGGTATTTGACCAACCAGGCCTGGGTGCAGGGCGTGCTGGATGGCAGTTACCGGTTGGAACGGAT
CGGCGCCTCGGCGTAA

Upstream 100 bases:

>100_bases
GACGCTTGGTTGACAATGCTTAGGTCAGGTATTGCTTCGACCGTATATCCTGTGTGGTTTGAAAACGCAGCGCGGTTCGC
GTGCGATCAGGAGATCAGCA

Downstream 100 bases:

>100_bases
CGCGCCACCGCAAGAGCCCGCGCAACCGTGTGTGGGCCTCACAAGGAACTATCTCATGACGCAACGTAAAGGCATTATTT
TGGCCGGTGGCTCGGGGACC

Product: dTDP-glucose 4,6-dehydratas

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MATWLVTGGAGFIGGNFVLEAVARGIRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGMLVARLLQEHQPDAVLNF
AAESHVDRSIEGPGAFIHTNVVGTLALLEAVRDYWKSLPTARSDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSA
SKAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLAK
GKVGETYNVGGNSERQNIEVVQAICALLDQHRPRDDGKPRASQITYVTDRPGHDRRYAIDASKLKNELGWEPSYTFEQGI
AQTVQWYLTNQAWVQGVLDGSYRLERIGASA

Sequences:

>Translated_351_residues
MATWLVTGGAGFIGGNFVLEAVARGIRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGMLVARLLQEHQPDAVLNF
AAESHVDRSIEGPGAFIHTNVVGTLALLEAVRDYWKSLPTARSDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSA
SKAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLAK
GKVGETYNVGGNSERQNIEVVQAICALLDQHRPRDDGKPRASQITYVTDRPGHDRRYAIDASKLKNELGWEPSYTFEQGI
AQTVQWYLTNQAWVQGVLDGSYRLERIGASA
>Mature_350_residues
ATWLVTGGAGFIGGNFVLEAVARGIRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIGDGMLVARLLQEHQPDAVLNFA
AESHVDRSIEGPGAFIHTNVVGTLALLEAVRDYWKSLPTARSDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSAS
KAASDHLVRAFHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLAKG
KVGETYNVGGNSERQNIEVVQAICALLDQHRPRDDGKPRASQITYVTDRPGHDRRYAIDASKLKNELGWEPSYTFEQGIA
QTVQWYLTNQAWVQGVLDGSYRLERIGASA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=36.7781155015198, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.8484848484848, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=25.3644314868805, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI56237023, Length=354, Percent_Identity=26.271186440678, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI56118217, Length=354, Percent_Identity=26.271186440678, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI189083684, Length=354, Percent_Identity=26.271186440678, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=59.7183098591549, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=58.7570621468927, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=27.8735632183908, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788366, Length=302, Percent_Identity=28.476821192053, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=35.3293413173653, Blast_Score=214, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=33.4319526627219, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=23.4604105571848, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=24.8603351955307, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=28.021978021978, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=24.6200607902736, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24158427, Length=292, Percent_Identity=26.027397260274, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38397; Mature: 38266

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATWLVTGGAGFIGGNFVLEAVARGIRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIG
CCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHEEECCCCCEEEECCCCCCEEEEEECCC
DGMLVARLLQEHQPDAVLNFAAESHVDRSIEGPGAFIHTNVVGTLALLEAVRDYWKSLPT
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
ARSDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSASKAASDHLVRAFHHTYGLPV
CCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLAK
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHHC
GKVGETYNVGGNSERQNIEVVQAICALLDQHRPRDDGKPRASQITYVTDRPGHDRRYAID
CCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEC
ASKLKNELGWEPSYTFEQGIAQTVQWYLTNQAWVQGVLDGSYRLERIGASA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
ATWLVTGGAGFIGGNFVLEAVARGIRVVNLDALTYAGNLNTLASLEGNPDHVFVKGDIG
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHEEECCCCCEEEECCCCCCEEEEEECCC
DGMLVARLLQEHQPDAVLNFAAESHVDRSIEGPGAFIHTNVVGTLALLEAVRDYWKSLPT
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
ARSDAFRFLHVSTDEVYGTLGETGKFTETTPYAPNSPYSASKAASDHLVRAFHHTYGLPV
CCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLVIAKALAGEPLPVYGDGKQVRDWLFVSDHCEAIRTVLAK
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHEEEHHHHHHHHHHHHC
GKVGETYNVGGNSERQNIEVVQAICALLDQHRPRDDGKPRASQITYVTDRPGHDRRYAID
CCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEC
ASKLKNELGWEPSYTFEQGIAQTVQWYLTNQAWVQGVLDGSYRLERIGASA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]