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The map label for this gene is maiA [H]

Identifier: 84622359

GI number: 84622359

Start: 754875

End: 755609

Strand: Direct

Name: maiA [H]

Synonym: XOO_0702

Alternate gene names: 84622359

Gene position: 754875-755609 (Clockwise)

Preceding gene: 84622358

Following gene: 84622361

Centisome position: 15.28

GC content: 64.08

Gene sequence:

>735_bases
ATGCCAGCGGCGTCAGCATCTTCGGCGCGATCGAACAGCGCGTTGAGCGGCAGCCGCTGCCATGACCGGTTGCTCACCTT
CCATGAGCGTCTGCAGTCAGGCTGGCGTATGAGCGCGCCCTATCGCGTGCGCATTGGGCTGGACATAAAGGCGCTGGCGT
ACGTCACGCACCCGGTGCATCTGGTGCGCGATGGCGGCGAGCAGCATGCCGCCGCGTACGCGCAGCTCAATCCACAGCAG
TTGGTTCCGACGCTGCGCCACGGCGCAGTGGTGGTTCCGCAGTCGCTGGCGATCCTGGAGTATCTGGAAGAAGCGTTTCC
ATACAGTGCGCGGCTGCTGCCGGCAGCACCGTCCGAACGCGCCCGTGTGCGCGCACTTGCCCAAGCCATCGCCTGCGATG
TGCACCTGCTCAATAACCTGCGCGTGATGCAGTTGCTCGAGCGCGAGTGGCAACGCGATGCCGCGCAGCGCCTGCACTGG
ATGCAGCACTGGATGCAGCAAGGGTTTGCGGCGCTGGAACTGCAGCTGGCCAACGAACCGTACACCGCAAGCTTCTGCCA
TGGCGACACGCCGGGCTTGGCCGATTGCGTGTTGATTCCGCAGCTCTATAACGCGCGCCGGTTCGAAGTAGATCTTGCGC
CTTATCCCACCCTGCAGCGTATTGAACAGGCCTGCCTGGCACTGCCGGCGTTCGATGCCGCGCGGCCGGAAATGCAGATT
GATGCAGTGGTGTGA

Upstream 100 bases:

>100_bases
TCGTGTTTTGCCGAACAGCGCGTGATCGAGACCCTGCGCGACGGCAAGCCGAGCACGCCATTCATGGCGTTCGGCGACGT
GGTGCGGATCGAGATGTTCG

Downstream 100 bases:

>100_bases
GGCGGAGGGTAAGAGAGGGTAGGTAGGACCCTCTCCTCTGTGCTGCGGGCAGCTTCACCGGTGCTGCGATAGCAAACGCG
GATTGTGCGCCGTTCTTTAA

Product: putative maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPAASASSARSNSALSGSRCHDRLLTFHERLQSGWRMSAPYRVRIGLDIKALAYVTHPVHLVRDGGEQHAAAYAQLNPQQ
LVPTLRHGAVVVPQSLAILEYLEEAFPYSARLLPAAPSERARVRALAQAIACDVHLLNNLRVMQLLEREWQRDAAQRLHW
MQHWMQQGFAALELQLANEPYTASFCHGDTPGLADCVLIPQLYNARRFEVDLAPYPTLQRIEQACLALPAFDAARPEMQI
DAVV

Sequences:

>Translated_244_residues
MPAASASSARSNSALSGSRCHDRLLTFHERLQSGWRMSAPYRVRIGLDIKALAYVTHPVHLVRDGGEQHAAAYAQLNPQQ
LVPTLRHGAVVVPQSLAILEYLEEAFPYSARLLPAAPSERARVRALAQAIACDVHLLNNLRVMQLLEREWQRDAAQRLHW
MQHWMQQGFAALELQLANEPYTASFCHGDTPGLADCVLIPQLYNARRFEVDLAPYPTLQRIEQACLALPAFDAARPEMQI
DAVV
>Mature_243_residues
PAASASSARSNSALSGSRCHDRLLTFHERLQSGWRMSAPYRVRIGLDIKALAYVTHPVHLVRDGGEQHAAAYAQLNPQQL
VPTLRHGAVVVPQSLAILEYLEEAFPYSARLLPAAPSERARVRALAQAIACDVHLLNNLRVMQLLEREWQRDAAQRLHWM
QHWMQQGFAALELQLANEPYTASFCHGDTPGLADCVLIPQLYNARRFEVDLAPYPTLQRIEQACLALPAFDAARPEMQID
AVV

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=41.9811320754717, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI22202622, Length=161, Percent_Identity=42.2360248447205, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=32.0754716981132, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17551302, Length=212, Percent_Identity=36.7924528301887, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17556142, Length=204, Percent_Identity=35.2941176470588, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17510461, Length=212, Percent_Identity=33.0188679245283, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45553325, Length=215, Percent_Identity=39.5348837209302, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21355859, Length=212, Percent_Identity=39.622641509434, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645375, Length=212, Percent_Identity=39.622641509434, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=37.2641509433962, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 27262; Mature: 27130

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAASASSARSNSALSGSRCHDRLLTFHERLQSGWRMSAPYRVRIGLDIKALAYVTHPVH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
LVRDGGEQHAAAYAQLNPQQLVPTLRHGAVVVPQSLAILEYLEEAFPYSARLLPAAPSER
EECCCCHHHHHHHHHCCHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCEECCCCCCHH
ARVRALAQAIACDVHLLNNLRVMQLLEREWQRDAAQRLHWMQHWMQQGFAALELQLANEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCC
YTASFCHGDTPGLADCVLIPQLYNARRFEVDLAPYPTLQRIEQACLALPAFDAARPEMQI
CEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEE
DAVV
EECC
>Mature Secondary Structure 
PAASASSARSNSALSGSRCHDRLLTFHERLQSGWRMSAPYRVRIGLDIKALAYVTHPVH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
LVRDGGEQHAAAYAQLNPQQLVPTLRHGAVVVPQSLAILEYLEEAFPYSARLLPAAPSER
EECCCCHHHHHHHHHCCHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCEECCCCCCHH
ARVRALAQAIACDVHLLNNLRVMQLLEREWQRDAAQRLHWMQHWMQQGFAALELQLANEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCC
YTASFCHGDTPGLADCVLIPQLYNARRFEVDLAPYPTLQRIEQACLALPAFDAARPEMQI
CEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEE
DAVV
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]