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The map label for this gene is tyrS [H]

Identifier: 84622137

GI number: 84622137

Start: 518935

End: 520146

Strand: Direct

Name: tyrS [H]

Synonym: XOO_0480

Alternate gene names: 84622137

Gene position: 518935-520146 (Clockwise)

Preceding gene: 84622134

Following gene: 84622142

Centisome position: 10.5

GC content: 61.96

Gene sequence:

>1212_bases
TTGACCACTCTCGACGAATCTCTCGCACTCATCGGCCGCGGCGCCGAAGAGATCCTCAAGCTCGATCAGCTCGAAGCACG
GCTGACATCAGGCGTGCCATTGCGGGTGAAGGCCGGCTTCGATCCCACCGCGCCGGATCTGCACCTGGGCCATACCGTGC
TGCTCAACAAGATGCGGCAGTTCCAGCAGCTCGGTCATCAGGTGATCTTCCTGATCGGCGACTTCACCGGAATGATCGGC
GACCCGAGCGGCAAGAACGCCACCCGCAAGCCGCTCAGCCGCGAGGATGTGCTGGCCAACGCACGGACTTATGAAGAGCA
GGTTTTCAAGATACTGGACCGCGAACGCACCGAAGTGCGCTTCAACTCCGAATGGTTCGGCCAGATGAGCGCGGCGGACA
TGATCAAGCTGTCCGCCCAGCACACGGTGGCGCGCATGCTCGAACGTGATGACTTCGCCAAGCGCTTCGGCAGCCAGCAG
CCGATCGCCATCCATGAATTCCTCTATCCATTGGTGCAGGGCTACGACTCGGTCGCGTTGAGGGCGGATGTCGAGCTCGG
CGGCACCGATCAGAAGTTCAACCTGTTGATGGGGCGCGGCCTGCAGGAACACTACGGTCAGGCACCACAGATCGTGCTGA
CCATGCCACTGCTGGAAGGCCTGGAGGGTGTCGCCAAGATGTCCAAGTCCCTGGGCAACTACATCGGCATCAACGAGCCG
GCCATCGACATCGTCACCAAGACCATGAAGATCGGCGACGAACTGACCTGGCGCTGGATCGATCTGCTGTCCCTCGACAT
CAGCGTGGCCGAAGCGGTGCGCCTGAAGGAGCAGGTCACCTCCGGTGAACTGCACCCGCGCGAGGTCAAATTGCGGCTGG
CGCGCGAATTGGCCACGCGGTTCCATGATGCCGCAACGGCAGAGCAGGCCATTGCCGGTTGGCATGCCGTGGTAACAGGG
CAGGGCGACACCAGCCTGCTGCCGCTGCAGGAAGTGGTGGTGCCGGCCGAAGGTTTGCGTATTGCCAGCCTGCTGACTGC
CGCCGGGCTGACGCCCAGCAATTCCGAAGCGACCCGTAAGCTCAAGGAACGCGCGGTCAAGATCGACGGTGAAGTGCTGG
AAGATGCCACCCGCGTCTTCACGCAGGGCTTTGAAGGCGTCATCCAAGTGGGCAAGCGCAATTTCGCCCGCGTGTCGCTT
GTCATTGGCTAA

Upstream 100 bases:

>100_bases
CAAACCATTGAACCTGTTCATTGATTTTCACTTGGCGCGAAATGAACCCGCGTATAACATCATTCACGTTGTTGACGGCA
AGCGCCGCTGGAGTCTGTGG

Downstream 100 bases:

>100_bases
AGCGCACTGAGGGTGCTGCAGGGCGGCAATTTCGGTGGCTCCACACGCCCTGCCGCTATATACATCTAGCGGTTTCAATG
CCCTCGAAGGTTTTTCGTCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTTLDESLALIGRGAEEILKLDQLEARLTSGVPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLEGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDELTWRWIDLLSLDISVAEAVRLKEQVTSGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTSLLPLQEVVVPAEGLRIASLLTAAGLTPSNSEATRKLKERAVKIDGEVLEDATRVFTQGFEGVIQVGKRNFARVSL
VIG

Sequences:

>Translated_403_residues
MTTLDESLALIGRGAEEILKLDQLEARLTSGVPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLEGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDELTWRWIDLLSLDISVAEAVRLKEQVTSGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTSLLPLQEVVVPAEGLRIASLLTAAGLTPSNSEATRKLKERAVKIDGEVLEDATRVFTQGFEGVIQVGKRNFARVSL
VIG
>Mature_402_residues
TTLDESLALIGRGAEEILKLDQLEARLTSGVPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIGD
PSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQP
IAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLEGVAKMSKSLGNYIGINEPA
IDIVTKTMKIGDELTWRWIDLLSLDISVAEAVRLKEQVTSGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTGQ
GDTSLLPLQEVVVPAEGLRIASLLTAAGLTPSNSEATRKLKERAVKIDGEVLEDATRVFTQGFEGVIQVGKRNFARVSLV
IG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=424, Percent_Identity=25.9433962264151, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787925, Length=402, Percent_Identity=27.6119402985075, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=211, Percent_Identity=32.7014218009479, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=390, Percent_Identity=24.8717948717949, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=24.6268656716418, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44471; Mature: 44340

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLDESLALIGRGAEEILKLDQLEARLTSGVPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLEGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDELTWRWIDLLSLDISVAEAVRLKEQVTSGELHPREVKLRLARELATR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTSLLPLQEVVVPAEGLRIASLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHH
LKERAVKIDGEVLEDATRVFTQGFEGVIQVGKRNFARVSLVIG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
TTLDESLALIGRGAEEILKLDQLEARLTSGVPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLEGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDELTWRWIDLLSLDISVAEAVRLKEQVTSGELHPREVKLRLARELATR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTSLLPLQEVVVPAEGLRIASLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHH
LKERAVKIDGEVLEDATRVFTQGFEGVIQVGKRNFARVSLVIG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]