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The map label for this gene is pcaB [H]

Identifier: 84622108

GI number: 84622108

Start: 489854

End: 491146

Strand: Direct

Name: pcaB [H]

Synonym: XOO_0451

Alternate gene names: 84622108

Gene position: 489854-491146 (Clockwise)

Preceding gene: 84622107

Following gene: 84622109

Centisome position: 9.92

GC content: 68.75

Gene sequence:

>1293_bases
ATGAGTGCGACATCTTCGCTATTGGGATCCTTGTTCGGCGAGCCTGCATGCGACGCCTTGTTTGACGATGCCGCGCGCGT
GCAGGCGATGCTGCAGTTCGAATCCGCGCTGGCGCGTGCGCAGGCGCAGTGCGGGGTGATTCCGACCGAGGCCGCGCAGG
CCATTGCCGCTGCCTGCGATGTGCGGCATCACGATCTGGCTGCGTTGGCACAGGCCACGGCGTTGGCCGGCAACCCCGCC
ATTCCGTTGGTCAAGGCGCTGACCGCCCAGGTGGCCGCGCACGACAGCGCTGCCGCGCGCTGGGTGCACTGGGGCGCTAC
CAGCCAGGACGTCCTCGATACCGGCACGGTGCTGCAGTTGCGTGCTGCGCTCGATCTGCTGCTGCCACGGCTGCACGCCT
TGTGCGCCGGGCTGGCCACATTGGCCGAACGCGAACGCGATACCGGCTTGCCTGGACGTACCTTGTTGCAGCAGGCCGTG
CCGGTCACCTTCGGGCTCAAGGTCGCCGCCTGGCTGGACGCATTGCAACGGGCGCATCAGCGCCTGCAGGCCATGGAGCA
CGATGCGCTGGTGCTGCACTTCGGCGGCGCCGCCGGCACGCTGGCTTCGCTCCAGGAGCGTGGCCTGGAAGTGGCGCAGG
CGCTGGCGAGCACTCTGGAGCTGCCGCTGCCCGCGCTGCCCTGGCATACGGCGCGCGACCGCATCGTTGAGGTCGGCTGC
GCGTTCGGTCTGCTGGCCGGCACGCTGGGCAAGATCGGCGGCGATGTGGTGTTGCTGATGCAGTCGGAGGTGGGCGAAGC
GTTCGAGCCCGCCGCTGCCGGCAAGGGCGGTTTGTCGGCAATGCCGCACAAGCGCAATCCGGTCTCCAGCGTTGCAGCGG
TTGCCGCGGCCACCCGCGTGCCCGGCCTGGTCGCCACCTTGTTCAGTGCCATGGCGCAGCCGCACGAGCGCGCGGCTGGG
CAATGGCATGCCGAATGGGACACCTTGCCGCAGATCGTCTGCCTCACCGCCGGCAGCTTGGCGCAGATGCAGCAGTGCCT
GTCTGGCCTGGAACTTGACCGCGCCCGCATGCGTATACATCTGGATAGCCACGGCAGGCTGCTCTACGCCGAGGCGGCGG
TATTCGCCCTTGCGCCGCAACTGGGCAAGCCGCAAGCGCATGCCCTGGTGCAACAGGCAGCGCTGCGCGCGCAGGCGCAG
CAACGGCATTTGCGCGAGGTGCTCGGCGAGGACGCGCAGGTGAGCGCCGTGCTCACGCACGCGCAACTGCAAGCCGTGTT
CGATAGCAGATAG

Upstream 100 bases:

>100_bases
ACCCCGGCAGTGGCAGGCGCGCACGGCACCTTGTCCTTTTGTCTAGTTGGCACCTTGGCACATCGGTGCGATGCTGCCCG
CACCCCTAGAGATGCAATGC

Downstream 100 bases:

>100_bases
TTGGCGCGGCATGTCCTCGGTCTGGATCGACCGCGTGCTGGCCGGATCTCCTTCCTGTTGACGGTGCTTCGATGGCTTAT
CTCCGGTTGCCCACGCATCG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSATSSLLGSLFGEPACDALFDDAARVQAMLQFESALARAQAQCGVIPTEAAQAIAAACDVRHHDLAALAQATALAGNPA
IPLVKALTAQVAAHDSAAARWVHWGATSQDVLDTGTVLQLRAALDLLLPRLHALCAGLATLAERERDTGLPGRTLLQQAV
PVTFGLKVAAWLDALQRAHQRLQAMEHDALVLHFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
AFGLLAGTLGKIGGDVVLLMQSEVGEAFEPAAAGKGGLSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAG
QWHAEWDTLPQIVCLTAGSLAQMQQCLSGLELDRARMRIHLDSHGRLLYAEAAVFALAPQLGKPQAHALVQQAALRAQAQ
QRHLREVLGEDAQVSAVLTHAQLQAVFDSR

Sequences:

>Translated_430_residues
MSATSSLLGSLFGEPACDALFDDAARVQAMLQFESALARAQAQCGVIPTEAAQAIAAACDVRHHDLAALAQATALAGNPA
IPLVKALTAQVAAHDSAAARWVHWGATSQDVLDTGTVLQLRAALDLLLPRLHALCAGLATLAERERDTGLPGRTLLQQAV
PVTFGLKVAAWLDALQRAHQRLQAMEHDALVLHFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
AFGLLAGTLGKIGGDVVLLMQSEVGEAFEPAAAGKGGLSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAG
QWHAEWDTLPQIVCLTAGSLAQMQQCLSGLELDRARMRIHLDSHGRLLYAEAAVFALAPQLGKPQAHALVQQAALRAQAQ
QRHLREVLGEDAQVSAVLTHAQLQAVFDSR
>Mature_429_residues
SATSSLLGSLFGEPACDALFDDAARVQAMLQFESALARAQAQCGVIPTEAAQAIAAACDVRHHDLAALAQATALAGNPAI
PLVKALTAQVAAHDSAAARWVHWGATSQDVLDTGTVLQLRAALDLLLPRLHALCAGLATLAERERDTGLPGRTLLQQAVP
VTFGLKVAAWLDALQRAHQRLQAMEHDALVLHFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGCA
FGLLAGTLGKIGGDVVLLMQSEVGEAFEPAAAGKGGLSAMPHKRNPVSSVAAVAAATRVPGLVATLFSAMAQPHERAAGQ
WHAEWDTLPQIVCLTAGSLAQMQQCLSGLELDRARMRIHLDSHGRLLYAEAAVFALAPQLGKPQAHALVQQAALRAQAQQ
RHLREVLGEDAQVSAVLTHAQLQAVFDSR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508577, Length=348, Percent_Identity=23.8505747126437, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323391, Length=332, Percent_Identity=25, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24647570, Length=328, Percent_Identity=28.9634146341463, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 45100; Mature: 44969

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSSLLGSLFGEPACDALFDDAARVQAMLQFESALARAQAQCGVIPTEAAQAIAAACD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VRHHDLAALAQATALAGNPAIPLVKALTAQVAAHDSAAARWVHWGATSQDVLDTGTVLQL
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHCCCHHHHH
RAALDLLLPRLHALCAGLATLAERERDTGLPGRTLLQQAVPVTFGLKVAAWLDALQRAHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RLQAMEHDALVLHFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
HHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AFGLLAGTLGKIGGDVVLLMQSEVGEAFEPAAAGKGGLSAMPHKRNPVSSVAAVAAATRV
HHHHHHHHHHHCCCCEEEEEEHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PGLVATLFSAMAQPHERAAGQWHAEWDTLPQIVCLTAGSLAQMQQCLSGLELDRARMRIH
CHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHEEEE
LDSHGRLLYAEAAVFALAPQLGKPQAHALVQQAALRAQAQQRHLREVLGEDAQVSAVLTH
ECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQLQAVFDSR
HHHHHHHCCC
>Mature Secondary Structure 
SATSSLLGSLFGEPACDALFDDAARVQAMLQFESALARAQAQCGVIPTEAAQAIAAACD
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VRHHDLAALAQATALAGNPAIPLVKALTAQVAAHDSAAARWVHWGATSQDVLDTGTVLQL
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHCCCHHHHH
RAALDLLLPRLHALCAGLATLAERERDTGLPGRTLLQQAVPVTFGLKVAAWLDALQRAHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RLQAMEHDALVLHFGGAAGTLASLQERGLEVAQALASTLELPLPALPWHTARDRIVEVGC
HHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AFGLLAGTLGKIGGDVVLLMQSEVGEAFEPAAAGKGGLSAMPHKRNPVSSVAAVAAATRV
HHHHHHHHHHHCCCCEEEEEEHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PGLVATLFSAMAQPHERAAGQWHAEWDTLPQIVCLTAGSLAQMQQCLSGLELDRARMRIH
CHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHEEEE
LDSHGRLLYAEAAVFALAPQLGKPQAHALVQQAALRAQAQQRHLREVLGEDAQVSAVLTH
ECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQLQAVFDSR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]