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The map label for this gene is pcaF [H]

Identifier: 84622104

GI number: 84622104

Start: 486770

End: 487978

Strand: Direct

Name: pcaF [H]

Synonym: XOO_0447

Alternate gene names: 84622104

Gene position: 486770-487978 (Clockwise)

Preceding gene: 84622102

Following gene: 84622105

Centisome position: 9.85

GC content: 67.58

Gene sequence:

>1209_bases
ATGAGCGATGTGTATCTGATCGATGGCATCCGCACGCCGATCGGCCGTTACGGTGGCGCCTTGTCCAGCGTGCGCGCTGA
CGACCTGGGTGCGGTGCCGATCGCCGCGCTGCTCGTGCGCCATCCGCAGATGGAGCCCGCCGCAATCGACGATGTGTACC
TGGGCTGCGCGAACCAGGCCGGTGAAGACAATCGCAATGTCGCGCGTATGAGTCTGTTGCTGGCCGGCGTGCCCTCTTCG
GTGCCGGGTAGCACGCTCAATCGCCTCTGCGGTTCGGGGCTGGACGCAGTCGGCACGGTGGCGCGTGGCATTCGTGCCGG
CGAACTGGATCTTGCGATTGCCGGCGGTGTCGAATCGATGTCGCGTGCGCCGTGGGTCATGGGCAAGGCCGACAGCGCCT
TCGCCCGCAACCAGCAGCTGGAAGACACCACCATGGGCTGGCGCTTCATCAATCCGCGCCTGCAAGCCGCCTATGGCACC
GAATTGATGGGCGAAACCGCCGAGAACCTCGCGCAGCTTCATGCCATCGCGCGCGAAGACCAGGATGCGTTTGCACTGCG
CAGTCAACAACGCGTGGCGGCGGCGCAGGCGGCCGGCTTCTTCGATGGAGAAATCACTTCGGTGGCAGTGGCCGGGCGCA
AAGGCGGCGATGCCAGCACCGTCGAATACGATGAGGCGCCGCGGCCCAACACCACGGCTGAGATGCTGGCCAAGCTCAAG
CCGGTGTTTCGCCAGCCCGGGACCGTGACGGCCGGCAATGCCTCCGGTCTCAACGATGGCGCCGCTGCCTTGCTGCTGGC
CTCTGCGCAGGCTGTGCAGGCGCATCGCCTGACGCCGCGTGCGCGGGTGCTGGGGTTCGCCTCGGCTGGCGTGGAGCCGG
CGTATATGGGCATCGGCCCGGTGCCGGCCACCCAGCGCCTGCTGGCGCGGTTGCACTTGCGCATCGCGCAGTTCGATGCG
ATCGAACTCAATGAAGCCTTTGCTGCACAGGCACTGGCCTGCACACGCGCATTCGGGCTGGCCGACGATGCCGCACACGT
CAATGCCAACGGTGGCGCCATCGCGCTGGGGCATCCGCTCGGCATGAGCGGCGCGCGGCTGGCGCTGACCCTGCTGCGGC
AGCTGGAAGCCAGCAACGGGCGACTCGGGCTGGCCAGCATGTGCATCGGGGTGGGGCAGGGCGTAGCGCTGGCGATCGAA
CGCCTGTGA

Upstream 100 bases:

>100_bases
CGCAGACCACCCCACCTGGCGCCACCGAGCTGGACGTCTTGCGCGCGCTGCAGGCACGCACCGATGCCGCGCATGATGCC
CAGGCGGCGGGAGCGGAGGC

Downstream 100 bases:

>100_bases
GCCGACTGTTGCAACCTACCTCGTCCTTCCCTTGCACCCATGCCGTTCTAGTCGCTTTGCTGCCGACGGAGATTCAAGAT
GCGTGATCCCACCTCTGACG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSDVYLIDGIRTPIGRYGGALSSVRADDLGAVPIAALLVRHPQMEPAAIDDVYLGCANQAGEDNRNVARMSLLLAGVPSS
VPGSTLNRLCGSGLDAVGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARNQQLEDTTMGWRFINPRLQAAYGT
ELMGETAENLAQLHAIAREDQDAFALRSQQRVAAAQAAGFFDGEITSVAVAGRKGGDASTVEYDEAPRPNTTAEMLAKLK
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQAHRLTPRARVLGFASAGVEPAYMGIGPVPATQRLLARLHLRIAQFDA
IELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEASNGRLGLASMCIGVGQGVALAIE
RL

Sequences:

>Translated_402_residues
MSDVYLIDGIRTPIGRYGGALSSVRADDLGAVPIAALLVRHPQMEPAAIDDVYLGCANQAGEDNRNVARMSLLLAGVPSS
VPGSTLNRLCGSGLDAVGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARNQQLEDTTMGWRFINPRLQAAYGT
ELMGETAENLAQLHAIAREDQDAFALRSQQRVAAAQAAGFFDGEITSVAVAGRKGGDASTVEYDEAPRPNTTAEMLAKLK
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQAHRLTPRARVLGFASAGVEPAYMGIGPVPATQRLLARLHLRIAQFDA
IELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEASNGRLGLASMCIGVGQGVALAIE
RL
>Mature_401_residues
SDVYLIDGIRTPIGRYGGALSSVRADDLGAVPIAALLVRHPQMEPAAIDDVYLGCANQAGEDNRNVARMSLLLAGVPSSV
PGSTLNRLCGSGLDAVGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARNQQLEDTTMGWRFINPRLQAAYGTE
LMGETAENLAQLHAIAREDQDAFALRSQQRVAAAQAAGFFDGEITSVAVAGRKGGDASTVEYDEAPRPNTTAEMLAKLKP
VFRQPGTVTAGNASGLNDGAAALLLASAQAVQAHRLTPRARVLGFASAGVEPAYMGIGPVPATQRLLARLHLRIAQFDAI
ELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEASNGRLGLASMCIGVGQGVALAIER
L

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=41.75, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI148539872, Length=401, Percent_Identity=40.1496259351621, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4501853, Length=405, Percent_Identity=40.4938271604938, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=38.0246913580247, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.8501170960187, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI194353979, Length=404, Percent_Identity=31.4356435643564, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=63.4328358208955, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI48994986, Length=410, Percent_Identity=44.8780487804878, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=46.4019851116625, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=46.2686567164179, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=31.7422434367542, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=42.1052631578947, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=35.25, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=409, Percent_Identity=34.2298288508557, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17535917, Length=406, Percent_Identity=30.2955665024631, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=30.3030303030303, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=36.9077306733167, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=36.2745098039216, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=46.2686567164179, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=39.7022332506204, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=35.9801488833747, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.3084112149533, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41624; Mature: 41493

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVYLIDGIRTPIGRYGGALSSVRADDLGAVPIAALLVRHPQMEPAAIDDVYLGCANQA
CCCEEEECCCCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEECCCCC
GEDNRNVARMSLLLAGVPSSVPGSTLNRLCGSGLDAVGTVARGIRAGELDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCHHHH
SRAPWVMGKADSAFARNQQLEDTTMGWRFINPRLQAAYGTELMGETAENLAQLHAIARED
HCCCEEECCCCHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDAFALRSQQRVAAAQAAGFFDGEITSVAVAGRKGGDASTVEYDEAPRPNTTAEMLAKLK
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHH
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQAHRLTPRARVLGFASAGVEPAYMGIGP
HHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCEECCCC
VPATQRLLARLHLRIAQFDAIELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPL
CCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCC
GMSGARLALTLLRQLEASNGRLGLASMCIGVGQGVALAIERL
CCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHEECC
>Mature Secondary Structure 
SDVYLIDGIRTPIGRYGGALSSVRADDLGAVPIAALLVRHPQMEPAAIDDVYLGCANQA
CCEEEECCCCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEECCCCC
GEDNRNVARMSLLLAGVPSSVPGSTLNRLCGSGLDAVGTVARGIRAGELDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCHHHH
SRAPWVMGKADSAFARNQQLEDTTMGWRFINPRLQAAYGTELMGETAENLAQLHAIARED
HCCCEEECCCCHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDAFALRSQQRVAAAQAAGFFDGEITSVAVAGRKGGDASTVEYDEAPRPNTTAEMLAKLK
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHH
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQAHRLTPRARVLGFASAGVEPAYMGIGP
HHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCEECCCC
VPATQRLLARLHLRIAQFDAIELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPL
CCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCC
GMSGARLALTLLRQLEASNGRLGLASMCIGVGQGVALAIERL
CCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]